Toxic effects of carbamazepine to embryonically exposed zebrafish during early life stage development

Embryonic zebrafish (Danio rerio) were exposed to 0, 1, 5, 10, or 50 micrograms per liter carbamazepine (CBZ) from 2 hours post fertilization (hpf) through hatching, and then sampled at 48, 72, or 144 hpf. Larvae collected at 48, 72, and 144 hpf were assessed for transcriptomic profiles using RNA sequencing. Larvae collected at 72 and 144 hpf time points were assessed for histological changes in the thickness of retinal layers using hematoxylin and eosin staining. Larvae collected at 144 hpf were assessed for optokinetic response (OKR), a visually-mediated behavioral assay that quantifies the number of eye movements while focusing on an external stimulus. The concentrations of CBZ in exposure media that embryos were exposed to were chemically validated using liquid chromatography-tandem mass spectroscopy. Sequence data are stored on the NCBI Sequence Read Archive (SRA, https://www.ncbi.nlm.nih.gov/sra), under BioProject accession number PRJNA1235748: Carbamazepine effects vision in early life stage zebrafish. Biosample accession numbers are SAMN47342737 - SAMN47342781.