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Publications

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Observational, virological, and serological data provide insights into an outbreak of highly pathogenic avian influenza among wild birds on the Yukon-Kuskokwim Delta, Alaska in 2022 Observational, virological, and serological data provide insights into an outbreak of highly pathogenic avian influenza among wild birds on the Yukon-Kuskokwim Delta, Alaska in 2022

In 2021–22, clade 2.3.4.4b highly pathogenic avian influenza (HPAI) viruses were introduced by wild birds into North America, leading to geographically widespread disease. In response to HPAI outbreaks throughout late 2021 and early 2022, we recorded observations of sick and dead birds, estimated abundance of carcasses, collected swab and sera samples to detect viruses, and monitored...
Authors
Bryan Daniels, Erik Osnas, Megan Boldenow, Robert Gerlach, Christina Ahlstrom, Sarah Coburn, Michael Brook, Michael Brubaker, Julian Fischer, David Koons, Angela Matz, Marin Murphy, Daniel Rizzolo, Laura Scott, David Sinnett, Jordan Thompson, Juliana Lenoch, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson, Andrew Ramey

U.S. Geological Survey science strategy to address highly pathogenic avian influenza and its effects on wildlife health 2025–29 U.S. Geological Survey science strategy to address highly pathogenic avian influenza and its effects on wildlife health 2025–29

Executive Summary Highly pathogenic avian influenza (HPAI) is an ecologically and economically important animal disease that can also directly affect humans (a “zoonotic” disease). HPAI was once limited almost exclusively to domestic poultry but has rapidly adapted to diverse animal hosts. Viruses causing HPAI now appear to be maintained and dispersed by wild birds largely independent of...
Authors
Andrew Ramey, Diann Prosser, Laura Hubbard, Guelaguetza Vazquez-Meves, Amy George, M. Hopkins

Detection of Giardia and Cryptosporidium in surface water of a subarctic city Detection of Giardia and Cryptosporidium in surface water of a subarctic city

Giardia and Cryptosporidium spp. are globally distributed protozoan parasites that can cause gastrointestinal disease in humans and animals. These zoonotic parasites and their ecological relationships have been understudied in Alaska and elsewhere, despite being identified as priority zoonotic pathogens. We aimed to detect and characterize Giardia and Cryptosporidium spp. in waterbodies...
Authors
Christina Ahlstrom, Michael Carey, Damian Menning, Jonathan O’Donnell, Andrew Ramey

Development of a genotyping-in-thousands by sequencing (GT-seq) panel for identifying individuals and estimating relatedness among Alaska black bears (Ursus americanus) Development of a genotyping-in-thousands by sequencing (GT-seq) panel for identifying individuals and estimating relatedness among Alaska black bears (Ursus americanus)

The management and conservation of large mammals, such as black bears (Ursus americanus), have long been informed by genetic estimates of population size and individual dispersal. Amplicon sequencing methods, also known as ‘genotyping-in-thousands-by sequencing’ (GT-seq), now enable the efficient and cost-effective genotyping of hundreds of loci and individuals in the same sequencing run...
Authors
Eleni Leto Petrou, Colette D. Brandt, Timothy J. Spivey, Kristen M. Gruenthal, Cherie Mckeeman, Sean Farley, David Battle, Cory Stantorf, Andrew Ramey

Bait trapping of waterfowl increases the environmental contamination of avian influenza virus (AIV) Bait trapping of waterfowl increases the environmental contamination of avian influenza virus (AIV)

Highly pathogenic avian influenza virus (HPAIV) H5Nx clade 2.3.4.4b has circulated in North America since late 2021, resulting in higher rates of morbidity and mortality in wild birds than observed in this region before. The objective of this study was to determine whether baiting, which is widely conducted in Canada and the United States as part of waterfowl management practices (e.g...
Authors
Cassandra Andrew, Landon McPhee, Kevin Kuchinski, Jordan Wight, Ishraq Rahman, Sarah Mansour, Gabrielle Angelo Cortez, Marzieh Kalhor, Ethan Kenmuir, Natalie Prystajecky, Kathryn Hargan, Andrew Lang, James Leafloor, Catherine Soos, Andrew Ramey, Chelsea Himsworth

Genomic characterization of highly pathogenic H5 avian influenza viruses from Alaska during 2022 provides evidence for genotype-specific trends of spatiotemporal and interspecies dissemination Genomic characterization of highly pathogenic H5 avian influenza viruses from Alaska during 2022 provides evidence for genotype-specific trends of spatiotemporal and interspecies dissemination

The ongoing panzootic of highly pathogenic H5 clade 2.3.4.4b avian influenza (HPAI) spread to North America in late 2021, with detections of HPAI viruses in Alaska beginning in April 2022. HPAI viruses have since spread across the state, affecting many species of wild birds as well as domestic poultry and wild mammals. To better understand the dissemination of HPAI viruses...
Authors
Christina Ahlstrom, Mia Torchetti, Julianna Lenoch, Kimberlee Beckmen, Megan Boldenow, Evan J Buck, Bryan Daniels, Krista Dilione, Robert Gerlach, Kristina Lantz, Angela Matz, Rebecca L. Poulson, Laura Scott, Gay Sheffield, David Sinnett, David E. Stallknecht, Raphaela Stimmelmayr, Eric Taylor, Alison Williams, Andrew Ramey

Molecular sexing of birds using quantitative PCR (qPCR) of sex-linked genes and logistic regression models Molecular sexing of birds using quantitative PCR (qPCR) of sex-linked genes and logistic regression models

The ability to sex individuals is an important component of many behavioural and ecological investigations and provides information for demographic models used in conservation and species management. However, many birds are difficult to sex using morphological characters or traditional molecular sexing methods. In this study, we developed probabilistic models for sexing birds using...
Authors
Eleni Leto Petrou, Laura Scott, Cherie Mckeeman, Andrew Ramey

Environmental DNA as a tool for better understanding the distribution, abundance, and health of Atlantic and Pacific salmon Environmental DNA as a tool for better understanding the distribution, abundance, and health of Atlantic and Pacific salmon

The development and application of approaches to detect and quantify environmental DNA (eDNA) have potential to improve our understanding of the distribution, abundance, and health of Atlantic Salmon Salmo salar and Pacific salmon Oncorhynchus spp. Here, we review 61 articles focusing on eDNA applications pertaining to salmon occupying natural habitat and aquaculture facilities in the...
Authors
Andrew Ramey, Cherie Mckeeman, Eleni Leto Petrou, Damian Menning, Ora Russ, Andres Lopez

Molecular detection and characterization of highly pathogenic H5N1 clade 2.3.4.4b avian influenza viruses among hunter-harvested wild birds provides evidence for three independent introductions into Alaska Molecular detection and characterization of highly pathogenic H5N1 clade 2.3.4.4b avian influenza viruses among hunter-harvested wild birds provides evidence for three independent introductions into Alaska

We detected and characterized highly pathogenic avian influenza viruses among hunter-harvested wild waterfowl inhabiting western Alaska during September–October 2022 using a molecular sequencing pipeline applied to RNA extracts derived directly from original swab samples. Genomic characterization of 10 H5 clade 2.3.4.4b avian influenza viruses detected with high confidence provided...
Authors
Andrew Ramey, Laura Scott, Christina Ahlstrom, Evan Buck, Alison Williams, Mia Kim Torchetti, David E. Stallknecht, Rebecca L. Poulson

The DDT-induced decline influenced genetic diversity in naturally-recovered peregrine falcons (Falco peregrinus) nesting within the Alaska Arctic and eastern Interior The DDT-induced decline influenced genetic diversity in naturally-recovered peregrine falcons (Falco peregrinus) nesting within the Alaska Arctic and eastern Interior

We assessed the influence of the severe mid-20th century population decline on genetic diversity in non-augmented peregrine falcon (Falco peregrinus) populations nesting within Alaska Arctic and eastern Interior. Microsatellite and mitochondrial DNA (mtDNA) data were analyzed for peregrine falcons sampled from three periods: pre-decline, decline, and post-decline. The influence of the...
Authors
Sarah Sonsthagen, Ted Swem, Skip Ambrose, Melanie Flamme, Clayton M White, George Sage, Sandra Talbot

Dynamic landscapes in northwestern North America structured populations of wolverines (Gulo gulo) Dynamic landscapes in northwestern North America structured populations of wolverines (Gulo gulo)

Cyclic climatic and glacial fluctuations of the Late Quaternary produced a dynamic biogeographic history for high latitudes. To refine our understanding of this history in northwestern North America, we explored geographic structure in a wide-ranging carnivore, the wolverine (Gulo gulo). We examined genetic variation in populations across mainland Alaska, coastal Southeast Alaska, and...
Authors
Dianna Krejsa, Sandra Talbot, George Sage, Sarah Sonsthagen, Thomas Jung, Audrey J Magoun, Joseph Cook

Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep Using next generation sequencing of alpine plants to improve fecal metabarcoding diet analysis for Dall’s sheep

Objectives Dall’s sheep (Ovis dalli dalli) are important herbivores in the mountainous ecosystems of northwestern North America, and recent declines in some populations have sparked concern. Our aim was to improve capabilities for fecal metabarcoding diet analysis of Dall’s sheep and other herbivores by contributing new sequence data for arctic and alpine plants. This expanded reference...
Authors
Kelly Williams, Damian Menning, Eric Wald, Sandra Talbot, Kumi Rattenbury, Laura R. Prugh
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