Protocols for the Detection of Renibacterium salmoninarum in salmon
Renibacterium salmoninarum is a fastidious, gram-positive bacterium and the causative agent of bacterial kidney disease (BKD) in wild and cultured salmonid fishes. A number of diagnostic tests exist for the direct and indirect detection of R. salmoninarum. These tests have different analytical properties and different abilities to quantify infection levels, detect remote infections and distinguish between live and dead bacteria. This website links to standard operating procedures (SOP) for the major R. salmoninarum diagnostic tests.
Quantitative Culture for R. salmoninarum - (2011) (Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid)
Enzyme linked immunosorbent assay (ELISA) for R. salmoninarum – (2012) (Double Polyclonal Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of Renibacterium salmoninarum)
Direct fluorescent antibody test (DFAT) – (2011) (Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues)
Membrane filtration fluorescent antibody test (MF-FAT) – (2012) (Membrane-Filtration Fluorescent Antibody Test (MF-FAT) for the detection of Renibacterium salmoninarum)
DNA extraction for gram positive bacteria – (2011) (DNA Extraction for PCR-based detection of Renibacterium salmoninarum)
Nested PCR (nPCR) test – (2011) (Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum)
Quantitative PCR (qPCR) test – (Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum)
- Overview
Protocols for the Detection of Renibacterium salmoninarum in salmon
Sources/Usage: Public Domain.Kidney lesions in a juvenile Chinook salmon (Oncorhynchus tshawytscha) affected by bacterial kidney disease (BKD). Credit: Caroline Bentley, PhD., University of Central Oklahoma. (Public domain.) Renibacterium salmoninarum is a fastidious, gram-positive bacterium and the causative agent of bacterial kidney disease (BKD) in wild and cultured salmonid fishes. A number of diagnostic tests exist for the direct and indirect detection of R. salmoninarum. These tests have different analytical properties and different abilities to quantify infection levels, detect remote infections and distinguish between live and dead bacteria. This website links to standard operating procedures (SOP) for the major R. salmoninarum diagnostic tests.
Sources/Usage: Public Domain.Renibacterium salmoninarum colonies growing on a spread plate inoculated for quantitative culture of the bacterium from fish tissues or ovarian fluid. (Public domain.) Quantitative Culture for R. salmoninarum - (2011) (Culture of Renibacterium salmoninarum from Tissues and Ovarian Fluid)
Sources/Usage: Public Domain.Double polyclonal enzyme-linked immunosorbent assay (ELISA) for the detection of Renibacterium salmonarium. Portion of a microplate showing green coloration in wells in which tissue samples containing high levels of R. salmoninarum antigens had been placed. (Public domain.) Enzyme linked immunosorbent assay (ELISA) for R. salmoninarum – (2012) (Double Polyclonal Enzyme-Linked Immunosorbent Assay (ELISA) for the detection of Renibacterium salmoninarum)
Sources/Usage: Public Domain.Direct fluorescent antibody test (DFAT) for the detection of Renibacterium salmoninarum in tissues. Fluorescing R. salmoninarum cells are visible on a slide. Credit: Carla Moanikeala Conway , USGS (Public domain.) Direct fluorescent antibody test (DFAT) – (2011) (Direct Fluorescent Antibody Test (DFAT) for the detection of Renibacterium salmoninarum in tissues)
Sources/Usage: Public Domain.Membrane-filtration fluorescent antibody test (MF-FAT) for the detection of Renibacterium salmoninarum. A sample is loaded onto a membrane filter held in a syringe-mounted holder. (Public domain.) Membrane filtration fluorescent antibody test (MF-FAT) – (2012) (Membrane-Filtration Fluorescent Antibody Test (MF-FAT) for the detection of Renibacterium salmoninarum)
Sources/Usage: Public Domain.DNA extraction for PCR-based detection of Renibacterium salmoninarum requires careful handling of samples to avoid contamination. (Public domain.) DNA extraction for gram positive bacteria – (2011) (DNA Extraction for PCR-based detection of Renibacterium salmoninarum)
Sources/Usage: Public Domain.Nested polymerase chain reaction (nPCR) for the detection of Renibacterium salmoninarum. Agarose gel electrophoresis is used for size separation and visualization of amplified DNA sequences. Credit: Debra Becker, USGS. (Public domain.) Nested PCR (nPCR) test – (2011) (Nested Polymerase Chain Reaction (nPCR) for the detection of Renibacterium salmoninarum)
Sources/Usage: Public Domain.Quantitative polymerase chain reaction (qPCR) assay for the detection of Renibacterium salmoninarum DNA. Observation of qPCR results on the screen of a sequence detection system. Credit: Debra Becker, USGS. (Public domain.) Quantitative PCR (qPCR) test – (Quantitative Polymerase Chain Reaction (qPCR) for the detection of Renibacterium salmoninarum)
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