Abundant ticks but limited Borrelia spp. pathogens in Mojave desert tortoise habitat

Ornithodoros ticks, a genus of soft ticks in the family Argasidae, occur worldwide including throughout deserts in the Southwestern United States. In the Mojave Desert, Ornithodoros parkeri and O. turicata are common ectoparasites of Mojave desert tortoises (Gopherus agassizii). These two tick species are vectors of Borrelia spp. that cause tick-borne relapsing fever (TBRF) in people. However, the distribution of O. parkeri and O. turicata and TBRF group Borrelia infection prevalence remain poorly known in the Mojave Desert. These ticks are commonly found in desert tortoise burrows. In this study, 1165 ticks were collected in the Mojave Desert, 1164 of those ticks were identified as belonging to the genus Ornithodoros, while the remaining tick was an Otobius species. We used morphologic characteristics and molecular genetic analysis to identify ticks as O. parkeri and O. turicata. Twenty-two of the 1165 tick samples tested positive for TBRF group Borrelia, five of which could be sequenced and were identified as Borrelia turicatae. This study sheds light on the distributions of tick species and pathogens in an understudied ecosystem, the Mojave Desert, and identifies group Borrelia as a potential risk factor in Mojave desert tortoise habitat.