Skip to main content
U.S. flag

An official website of the United States government

Acetylene fuels TCE reductive dechlorination by defined Dehalococcoides/Pelobacter consortia

January 11, 2017

Acetylene (C2H2) can be generated in contaminated groundwater sites as a consequence of chemical degradation of trichloroethene (TCE) by in situ minerals, and C2H2 is known to inhibit bacterial dechlorination. In this study, we show that while high C2H2 (1.3 mM) concentrations reversibly inhibit reductive dechlorination of TCE by Dehalococcoides mccartyi isolates as well as enrichment cultures containing D. mccartyi sp., low C2H2 (0.4 mM) concentrations do not inhibit growth or metabolism of D. mccartyi. Cocultures of Pelobacter SFB93, a C2H2-fermenting bacterium, with D. mccartyi strain 195 or with D. mccartyi strain BAV1 were actively sustained by providing acetylene as the electron donor and carbon source while TCE or cis-DCE served as the electron acceptor. Inhibition by acetylene of reductive dechlorination and methanogenesis in the enrichment culture ANAS was observed, and the inhibition was removed by adding Pelobacter SFB93 into the consortium. Transcriptomic analysis of D. mccartyi strain 195 showed genes encoding for reductive dehalogenases (e.g., tceA) were not affected during the C2H2-inhibition, while genes encoding for ATP synthase, biosynthesis, and Hym hydrogenase were down-regulated during C2H2 inhibition, consistent with the physiological observation of lower cell yields and reduced dechlorination rates in strain 195. These results will help facilitate the optimization of TCE-bioremediation at contaminated sites containing both TCE and C2H2.

Citation Information

Publication Year 2017
Title Acetylene fuels TCE reductive dechlorination by defined Dehalococcoides/Pelobacter consortia
DOI 10.1021/acs.est.6b05770
Authors Xinwei Mao, Ronald S. Oremland, Tong Liu, Abigail A Landers, Shaun Baesman, Lisa Alvarez-Cohen
Publication Type Article
Publication Subtype Journal Article
Series Title Environmental Science & Technology
Index ID 70181010
Record Source USGS Publications Warehouse
USGS Organization National Research Program - Western Branch

Related Content