Juvenile coho salmon (Oncorhynchus kisutch) from three populations in Washington State were captured in the field and tested for critical thermal maximum (CTM). Tolerances varied among the populations (mean CTMs were 28.21, 29.13, and 29.23 °C) and exceeded published data from some laboratory tests. The population from a relatively cool stream had a lower CTM than the two populations from warmer streams. However, after the salmon had been in the laboratory for 3 months under constant, common temperature regimes, the CTMs no longer differed, indicating that the population-specific differences resulted from different acclimation regimes rather than from genetic adaptation.