The efficacy and residual toxicity of a sodium hydroxide (NaOH) based ballast water treatment system (BWTS) were tested aboard the Great Lakes carrier M/V American Spiritin 1000 L mesocosms containing water from the ship's ballast tanks. NaOH was added to elevate the pH to 11.5 or 11.7 for 48 h, after which pH was reduced to