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Environmental DNA sampling protocol - filtering water to capture DNA from aquatic organisms

September 29, 2015

Environmental DNA (eDNA) analysis is an effective method of determining the presence of aquatic organisms such as fish, amphibians, and other taxa. This publication is meant to guide researchers and managers in the collection, concentration, and preservation of eDNA samples from lentic and lotic systems. A sampling workflow diagram and three sampling protocols are included as well as a list of suggested supplies. Protocols include filter and pump assembly using: (1) a hand-driven vacuum pump, ideal for sample collection in remote sampling locations where no electricity is available and when equipment weight is a primary concern; (2) a peristaltic pump powered by a rechargeable battery-operated driver/drill, suitable for remote sampling locations when weight consideration is less of a concern; (3) a 120-volt alternating current (AC) powered peristaltic pump suitable for any location where 120-volt AC power is accessible, or for roadside sampling locations. Images and detailed descriptions are provided for each step in the sampling and preservation process.

Citation Information

Publication Year 2015
Title Environmental DNA sampling protocol - filtering water to capture DNA from aquatic organisms
DOI 10.3133/tm2A13
Authors Matthew B. Laramie, David S. Pilliod, Caren S. Goldberg, Katherine M. Strickler
Publication Type Report
Publication Subtype USGS Numbered Series
Series Title Techniques and Methods
Series Number 2-A13
Index ID tm2A13
Record Source USGS Publications Warehouse
USGS Organization Forest and Rangeland Ecosystem Science Center