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Infectious hematopoietic necrosis virus detected by separation and incubation of cells from salmonid cavity fluid.

October 21, 2015

Infectious hematopoietic necrosis (IHN) virus is usually detected by inoculating susceptible cell cultures with cavity ("ovarian") fluid (CF) from spawning females. We identified additional adult carriers of virus in spawning populations of steelhead trout (Salmo gairdneri) and sockeye salmon (Oncorhynchus nerka) by collecting nonerythrocytic cells from CF samples by low-speed centrifugation, culturing the cells for at least 7 d at 15 °C, and then testing the culture medium for virus. Virus appeared in the cultured cells from some samples of CF that remained negative during incubation. In additional samples of CF from these species, the virus titer increased in cultured cells compared with the titer in the original CF sample. With chinook salmon (O.tshawytscha), no negative samples converted to positive during incubation, but the virus titer was retained in incubated CF cells, but not in cell-free CF.

Citation Information

Publication Year 1987
Title Infectious hematopoietic necrosis virus detected by separation and incubation of cells from salmonid cavity fluid.
DOI 10.1139/f87-126
Authors D. Mulcahy, W.N. Batts
Publication Type Article
Publication Subtype Journal Article
Series Title Canadian Journal of Fisheries and Aquatic Sciences
Series Number
Index ID 70162120
Record Source USGS Publications Warehouse
USGS Organization Alaska Science Center