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Large-scale dialysis of sample lipids

December 31, 1993

The use of a semipermeable membrane device (SPMD) for dialysis in an organic solvent phase is an efficient alternative approach to separation of contaminants from large amounts of lipid (up to 50 grams or more) prior to organic chemical analysis. Passive separation of contaminants can be accomplished with a minimum of equipment and a comparatively small volume of solvent. This study examines the effects of factors such as dialytic solvent, lipid type, dialytic solvent:lipid volume ratio, dialysis time, and temperature on the performance of polyethylene SPMDs during lipid-contaminant separations. The experimental conditions for maximal recoveries of organochlorine pesticides and polychlorinated biphenyls with minimal lipid carryover are determined for the examined variables. When the dialytic procedure is optimized, very satisfactory and highly reproducible analyte recoveries can be obtained in a few days while separating > 90% of the lipid material in a single operation.

Publication Year 1993
Title Large-scale dialysis of sample lipids
DOI 10.1016/0045-6535(93)90026-2
Authors Jill Meadows, Donald E. Tillitt, James Huckins, D. Schroeder
Publication Type Article
Publication Subtype Journal Article
Series Title Chemosphere
Index ID 70193867
Record Source USGS Publications Warehouse
USGS Organization Columbia Environmental Research Center