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Loading capacity and chromatographic behavior of a porous graphitic carbon column for polychlorinated biphenyls

January 1, 1998

A porous graphitic carbon column (Hypercarb) was used for the fractionation of polychlorinated biphenyls (PCBs) into classes of 2-4 ortho chlorines, 1 ortho chlorine and 0 ortho chlorine congeners. A method was developed that combined the fractionation of PCBs, polychlorinated dibenzo-p-dioxins and dibenzofurans in a variety of biotic environmental samples. Many of these samples have high concentrations of PCBs which cause fractionation problems as adsorption sites on the graphitic surface are occupied. The loading capacity of the column for PCBs was determined by injecting up to 1 mg of total PCBs and monitoring changes in chromatographic behavior of tetra-/di-ortho, mono-ortho and non-ortho substituted PCBs. Effective loading capacities were 1 mg for tetra-/di-ortho PCBs, but only 3–5 μg for non-ortho PCBs and about 2 μg for mono-ortho PCBs. Loading capacity of the PGC column for environmental fish and avian egg samples was determined to depend on the mono-ortho and non-ortho PCB levels found in these samples.

Citation Information

Publication Year 1998
Title Loading capacity and chromatographic behavior of a porous graphitic carbon column for polychlorinated biphenyls
DOI 10.1016/S0021-9673(98)00241-6
Authors K.R. Echols, R.W. Gale, K. Feltz, J. O'Laughlin, D.E. Tillitt, T.R. Schwartz
Publication Type Article
Publication Subtype Journal Article
Series Title Journal of Chromatography A
Series Number
Index ID 70020468
Record Source USGS Publications Warehouse
USGS Organization Columbia Environmental Research Center