Skip to main content
U.S. flag

An official website of the United States government

A minimally invasive method for extraction of sturgeon oocytes

July 14, 2010

Fishery biologists, hatchery personnel, and caviar fishers routinely extract oocytes from sturgeon (Acipenseridae) to determine the stage of maturation by checking egg quality. Typically, oocytes are removed either by inserting a catheter into the oviduct or by making an incision in the body cavity. Both methods can be time-consuming and stressful to the fish. We describe a device to collect mature oocytes from sturgeons quickly and effectively with minimal stress on the fish. The device is made by creating a needle from stainless steel tubing and connecting it to a syringe with polyvinyl chloride tubing. The device is filled with saline solution or water, the needle is inserted into the abdominal wall, and eggs are extracted from the fish. Using this device, an oocyte sample can be collected in less than 30 s. Such sampling leaves a minute wound that heals quickly and does not require suturing. The extractor device can easily be used in the field or hatchery, reduces fish handling time, and minimizes stress.

Citation Information

Publication Year 2010
Title A minimally invasive method for extraction of sturgeon oocytes
DOI 10.1577/A09-006.1
Authors James S. Candrl, Diana M. Papoulias, Donald E. Tillitt
Publication Type Article
Publication Subtype Journal Article
Series Title North American Journal of Aquaculture
Series Number
Index ID 70189128
Record Source USGS Publications Warehouse
USGS Organization Columbia Environmental Research Center