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Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM

March 16, 2021

A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass (Micropterus dolomieu) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the analysis of smallmouth bass total plasma IgM using ELISA, the mAb was found to be effective when used in an inhibition kinetic assay.

Citation Information

Publication Year 2021
Title Production and characterization of a mouse monoclonal antibody against smallmouth bass (Micropterus dolomieu) IgM
DOI 10.1016/j.fsi.2021.03.006
Authors Christopher A. Ottinger, Cheyenne R. Smith, Vicki S. Blazer, Luke R. Iwanowicz, Mary Ann Vogelbein, Stephen Kaattari
Publication Type Article
Publication Subtype Journal Article
Series Title Fish & Shellfish Immunology
Index ID 70218999
Record Source USGS Publications Warehouse
USGS Organization Leetown Science Center; Eastern Ecological Science Center