Donald Tillitt, PhD (Former Employee)
Science and Products
Thiamine (Vitamin B1) Deficiency in Fish and Wildlife
In the last few decades, thiamine deficiency has been observed in fish and wildlife in the Great Lakes, Alaska, Sweden and several other areas in North America and Europe. Thiamine is an essential vitamin needed for cell function. Thiamine deficiency has been linked to neurological disorders, immunosuppression, and lower reproductive viability. These disorders potentially reduce populations of...
Effects of estrogens and atrazine on functional immune responses of largemouth bass (Micropterus salmoides) Effects of estrogens and atrazine on functional immune responses of largemouth bass (Micropterus salmoides)
Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of bass in the Chesapeake Bay watershed. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural lands, and phytoestrogens from cover crops have been implicated as potential causes of these...
Alternative complement pathway assay data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Alternative complement pathway assay data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture
Alternative complement pathway assay data observed in adult largemouth bass plasma in response to an EE2 (17alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions, with associated fish identification information.
Egg thiamine content, length, weight and survival of steelhead (Oncorhynchus mykiss) exposed to three methods of experimental thiamine supplementation in 2022 and egg thiamine content from three Oregon hatcheries in 2019 Egg thiamine content, length, weight and survival of steelhead (Oncorhynchus mykiss) exposed to three methods of experimental thiamine supplementation in 2022 and egg thiamine content from three Oregon hatcheries in 2019
Early rearing of steelhead (Oncorhynchus mykiss) in Oregon hatcheries is often problematic; fry can become emaciated and die during the period between hatch and first feed. Thiamine (vitamin B1) deficiency has caused early mortality in salmonids; however, thiamine status of Oregon’s anadromous steelhead populations is currently unknown. We sampled eggs of 7-10 females during spawning...
Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS
This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure...
Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture
Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter)...
Dissolved organic carbon, total petroleum hydrocarbons and and toxicity assay results for Bemidji, MN (2018) Dissolved organic carbon, total petroleum hydrocarbons and and toxicity assay results for Bemidji, MN (2018)
In crude-oil-contaminant plumes the dissolved organic carbon (DOC) is mainly hydrocarbon degradation intermediates only partly quantified by the diesel range total petroleum hydrocarbon (TPHd) method. To understand potential biological effects of degradation intermediates we tested three fractions of DOC: (1) solid phase extract (HLB); (2) dichloromethane (DCM-total) extract used in TPHd...
Filter Total Items: 215
Widespread thiamine deficiency in California salmon linked to an anchovy-dominated marine prey base Widespread thiamine deficiency in California salmon linked to an anchovy-dominated marine prey base
Thiamine (vitamin B1) deficiency in marine systems is a globally significant threat to marine life. In 2020, newly hatched Chinook salmon (Oncorhynchus tshawytscha) fry in California’s Central Valley (CCV) hatcheries swam in corkscrew patterns and died at unusually high rates due to a lack of this essential vitamin. We subsequently investigated the impacts and causes of thiamine...
Authors
Nate Mantua, Heather M. Bell, Anne E. Todgham, Miles E. Daniels, Jacques Rinchard, Jarrod R. Ludwig, John Field, Steven T Lindley, Freya Elizabeth Rowland, Catherine A. Richter, David Walters, Bruce P. Finney, Anne R. Distajo Haskell, Donald Tillitt, Dale C. Honeyfield, Taylor N. Lipscomb, Kevin Kwak, Jason Kindopp, Dennis E. Cocherell, Abigail Ward, Thomas H. Williams, Jeff Harding, Nann A. Fangue, Carson Jeffres, Rocio Iliana Ruiz-Cooley, Steven Litvin, Scott Foott, Mark Adkison, Brett Kormos, Peggy Harte, Frederick S. Colwell, Christopher P. Suffridge, Kelly Shannon, Amanda Cranford, Charlotte Ambrose, Aimee N. Reed, Rachel C. Johnson
Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence
Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of Centrarchids in watersheds throughout the United States. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural land use, and phytoestrogens have been implicated as potential causes of...
Authors
Jessica Kristin Leet, Catherine A. Richter, Rachel Claunch, Robert Gale, Donald E. Tillitt, Luke R. Iwanowicz
Evolutionary and ecological correlates of thiaminase in fishes Evolutionary and ecological correlates of thiaminase in fishes
Thiamine (vitamin B1) is required by all living organisms in multiple metabolic pathways. It is scarce in natural systems, and deficiency can lead to reproductive failure, neurological issues, and death. One major cause of thiamine deficiency is an overreliance on diet items containing the enzyme thiaminase. Thiaminase activity has been noted in many prey fishes and linked to cohort...
Authors
Freya Elizabeth Rowland, Cathy A. Richter, Donald E Tillitt, David Walters
Reevaluation of 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalency factors for dioxin-like polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and polychlorinated biphenyls for fishes Reevaluation of 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalency factors for dioxin-like polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and polychlorinated biphenyls for fishes
An expert meeting was organized by the World Health Organization (WHO) in 1997 to streamline assessments of risk posed by mixtures of dioxin-like chemicals (DLCs) through development of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) equivalency factors (TEFs) for mammals, birds, and fishes. No reevaluation has been performed for fish TEFs. Therefore, the objective of the present...
Authors
Jon A. Doering, Donald E. Tillitt, Steve Wiseman
Factors influencing egg thiamine concentrations of Lake Ontario lake trout: 2019–2020 Factors influencing egg thiamine concentrations of Lake Ontario lake trout: 2019–2020
In the Great Lakes region, thiamine deficiency is considered a recruitment bottleneck for lake trout Salvelinus namaycush and has been correlated with the consumption of non-native alewife Alosa pseudoharengus. While alewife, the most abundant forage fish in Lake Ontario, are the predominant prey for lake trout, they also consume benthic prey such as round goby Neogobius melanostomus...
Authors
Aaron Heisey, Christopher Osborne, Brian F. Lantry, Donald E. Tillitt, Jacques Rinchard
Understanding and mitigating thiaminase activity in silver carp Understanding and mitigating thiaminase activity in silver carp
A deficiency of thiamine (vitamin B1), an essential cofactor for enzymes involved in metabolic processes, can be caused by the enzyme thiaminase. Thiaminase in food stocks has been linked to morbidity and mortality due to thiamine depletion in many ecologically and economically important species. Thiaminase activity has been detected in certain bacteria, plants, and fish species...
Authors
Patricia C. Wolfe, Amber M. Tuske, Donald E. Tillitt, Fred Allen, Katie A. Edwards
Science and Products
Thiamine (Vitamin B1) Deficiency in Fish and Wildlife
In the last few decades, thiamine deficiency has been observed in fish and wildlife in the Great Lakes, Alaska, Sweden and several other areas in North America and Europe. Thiamine is an essential vitamin needed for cell function. Thiamine deficiency has been linked to neurological disorders, immunosuppression, and lower reproductive viability. These disorders potentially reduce populations of...
Effects of estrogens and atrazine on functional immune responses of largemouth bass (Micropterus salmoides) Effects of estrogens and atrazine on functional immune responses of largemouth bass (Micropterus salmoides)
Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of bass in the Chesapeake Bay watershed. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural lands, and phytoestrogens from cover crops have been implicated as potential causes of these...
Alternative complement pathway assay data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Alternative complement pathway assay data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture
Alternative complement pathway assay data observed in adult largemouth bass plasma in response to an EE2 (17alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions, with associated fish identification information.
Egg thiamine content, length, weight and survival of steelhead (Oncorhynchus mykiss) exposed to three methods of experimental thiamine supplementation in 2022 and egg thiamine content from three Oregon hatcheries in 2019 Egg thiamine content, length, weight and survival of steelhead (Oncorhynchus mykiss) exposed to three methods of experimental thiamine supplementation in 2022 and egg thiamine content from three Oregon hatcheries in 2019
Early rearing of steelhead (Oncorhynchus mykiss) in Oregon hatcheries is often problematic; fry can become emaciated and die during the period between hatch and first feed. Thiamine (vitamin B1) deficiency has caused early mortality in salmonids; however, thiamine status of Oregon’s anadromous steelhead populations is currently unknown. We sampled eggs of 7-10 females during spawning...
Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS Quantitation of estrogens in water, alone and in combination with atrazine, using two chemically-activated luciferase expression, CALUX, bioassays and quadrupole-time of flight ultraperformance liquid chromatography/mass spectrometry, UPLC-MS
This data describes the relative luminescence units and relative fluorescence units of lysates of two estrogen responsive chemically-activated luciferase expression human breast VM7Luc4E2 and VM7LucERbc9 cells which were exposed for 24 hours to estrone and 17beta-estradiol dilutions alone and in mixture with atrazine or water extracts collected from dilutor tanks during a fish exposure...
Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture Water chemistry and fish metrics data for adult largemouth bass exposed in outdoor ponds to 17alpha-ethinylestradiol or an estrone-atrazine mixture
Physiological endpoints observed in adult largemouth bass in response to an EE2 (17 alpha-ethinylestradiol) or mixture of estrone and atrazine exposure under laboratory pond conditions. Also included are water quality and chemical concentration data. Adult LMB were exposed in outdoor pond mesocosms from post-spawning to early gonad recrudescence to either EE2 (2.4 nanograms per liter)...
Dissolved organic carbon, total petroleum hydrocarbons and and toxicity assay results for Bemidji, MN (2018) Dissolved organic carbon, total petroleum hydrocarbons and and toxicity assay results for Bemidji, MN (2018)
In crude-oil-contaminant plumes the dissolved organic carbon (DOC) is mainly hydrocarbon degradation intermediates only partly quantified by the diesel range total petroleum hydrocarbon (TPHd) method. To understand potential biological effects of degradation intermediates we tested three fractions of DOC: (1) solid phase extract (HLB); (2) dichloromethane (DCM-total) extract used in TPHd...
Filter Total Items: 215
Widespread thiamine deficiency in California salmon linked to an anchovy-dominated marine prey base Widespread thiamine deficiency in California salmon linked to an anchovy-dominated marine prey base
Thiamine (vitamin B1) deficiency in marine systems is a globally significant threat to marine life. In 2020, newly hatched Chinook salmon (Oncorhynchus tshawytscha) fry in California’s Central Valley (CCV) hatcheries swam in corkscrew patterns and died at unusually high rates due to a lack of this essential vitamin. We subsequently investigated the impacts and causes of thiamine...
Authors
Nate Mantua, Heather M. Bell, Anne E. Todgham, Miles E. Daniels, Jacques Rinchard, Jarrod R. Ludwig, John Field, Steven T Lindley, Freya Elizabeth Rowland, Catherine A. Richter, David Walters, Bruce P. Finney, Anne R. Distajo Haskell, Donald Tillitt, Dale C. Honeyfield, Taylor N. Lipscomb, Kevin Kwak, Jason Kindopp, Dennis E. Cocherell, Abigail Ward, Thomas H. Williams, Jeff Harding, Nann A. Fangue, Carson Jeffres, Rocio Iliana Ruiz-Cooley, Steven Litvin, Scott Foott, Mark Adkison, Brett Kormos, Peggy Harte, Frederick S. Colwell, Christopher P. Suffridge, Kelly Shannon, Amanda Cranford, Charlotte Ambrose, Aimee N. Reed, Rachel C. Johnson
Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence Immunomodulation in adult largemouth bass (Micropterus salmoides) exposed to a model estrogen or mixture of endocrine disrupting contaminants during early gonadal recrudescence
Disease outbreaks, skin lesions, fish kill events, and reproductive abnormalities have been observed in wild populations of Centrarchids in watersheds throughout the United States. Occurrence of synthetic and natural hormones from wastewater treatment plants and livestock operations, pesticides from agricultural land use, and phytoestrogens have been implicated as potential causes of...
Authors
Jessica Kristin Leet, Catherine A. Richter, Rachel Claunch, Robert Gale, Donald E. Tillitt, Luke R. Iwanowicz
Evolutionary and ecological correlates of thiaminase in fishes Evolutionary and ecological correlates of thiaminase in fishes
Thiamine (vitamin B1) is required by all living organisms in multiple metabolic pathways. It is scarce in natural systems, and deficiency can lead to reproductive failure, neurological issues, and death. One major cause of thiamine deficiency is an overreliance on diet items containing the enzyme thiaminase. Thiaminase activity has been noted in many prey fishes and linked to cohort...
Authors
Freya Elizabeth Rowland, Cathy A. Richter, Donald E Tillitt, David Walters
Reevaluation of 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalency factors for dioxin-like polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and polychlorinated biphenyls for fishes Reevaluation of 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalency factors for dioxin-like polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans, and polychlorinated biphenyls for fishes
An expert meeting was organized by the World Health Organization (WHO) in 1997 to streamline assessments of risk posed by mixtures of dioxin-like chemicals (DLCs) through development of 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) equivalency factors (TEFs) for mammals, birds, and fishes. No reevaluation has been performed for fish TEFs. Therefore, the objective of the present...
Authors
Jon A. Doering, Donald E. Tillitt, Steve Wiseman
Factors influencing egg thiamine concentrations of Lake Ontario lake trout: 2019–2020 Factors influencing egg thiamine concentrations of Lake Ontario lake trout: 2019–2020
In the Great Lakes region, thiamine deficiency is considered a recruitment bottleneck for lake trout Salvelinus namaycush and has been correlated with the consumption of non-native alewife Alosa pseudoharengus. While alewife, the most abundant forage fish in Lake Ontario, are the predominant prey for lake trout, they also consume benthic prey such as round goby Neogobius melanostomus...
Authors
Aaron Heisey, Christopher Osborne, Brian F. Lantry, Donald E. Tillitt, Jacques Rinchard
Understanding and mitigating thiaminase activity in silver carp Understanding and mitigating thiaminase activity in silver carp
A deficiency of thiamine (vitamin B1), an essential cofactor for enzymes involved in metabolic processes, can be caused by the enzyme thiaminase. Thiaminase in food stocks has been linked to morbidity and mortality due to thiamine depletion in many ecologically and economically important species. Thiaminase activity has been detected in certain bacteria, plants, and fish species...
Authors
Patricia C. Wolfe, Amber M. Tuske, Donald E. Tillitt, Fred Allen, Katie A. Edwards
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government