Dorothy is a microbiologist at the Western Fisheries Research Center.
Science and Products
Environmental DNA (eDNA) is an Effective Tool to Track Recolonizing Migratory Fish Following Large-Scale Dam Removal, field data
We collected environmental DNA (eDNA) data from the Elwha River, home to the world?s largest dam removal project, to track the spatial and temporal patterns of species responses following dam removal. In total, we collected data for 11 different fish taxa, sampled at 25 sites ranging across 56 river kilometers in a wilderness river for 4 years following dam removal. We show that eDNA can effective
Detection of invasive aquatic plants Myriophyllum spicatum and Egeria densa in lakes using eDNA, field and mesocosm data
We conducted a study to test the factors related to detectability of two invasive aquatic plants (Egeria densa and Myriophyllym spicatum) using environmental DNA (eDNA), over extended periods of time, and specifically examined how plant growth stage and abundance relates to eDNA detection in semi-natural and natural conditions. This dataset is from sampling performed in summer of 2018 in lakes wit
Distribution and seasonal differences in Pacific Lamprey and Lampetra spp eDNA across 18 Puget Sound watersheds, 2014 and 2015
These data contain all the raw results needed to support the conclusions for the final product. These data are water sampling locations (latitude and longitude), date of water sampling, quantitative PCR values for each water sample, and stream flow at USGS stream gauging stations on sampling day.
Filter Total Items: 15
Ontogeny of eDNA shedding during early development in Chinook Salmon (Oncorhynchus tshawytscha)
Knowledge of the timing of major life history events in aquatic species is important for informing conservation and resource management planning. Accordingly, surveys of environmental DNA (eDNA) have been performed to determine the efficacy of eDNA for providing information on life history events, primarily focusing on the timing of events associated with spawning, and these studies have proved su
Authors
Carl Ostberg, Dorothy M. Chase
Environmental DNA is an effective tool to track recolonizing migratory fish following large‐scale dam removal
Environmental DNA (eDNA) has emerged as a potentially powerful tool for use in conservation and resource management, including for tracking the recolonization dynamics of fish populations. We used eDNA to assess the effectiveness of dam removal to restore fish passage on the Elwha River in Washington State (USA). Using a suite of 11 species‐specific eDNA polymerase chain reaction (PCR) assays, we
Authors
Jeffrey J. Duda, Marshal Hoy, Dorothy M. Chase, George R. Pess, Samuel J. Brenkman, Michael M McHenry, Carl Ostberg
Use of environmental DNA to detect the invasive aquatic plants Myriophyllum spicatum and Egeria densa in lakes
Environmental DNA (eDNA) analysis offers a promising tool for rapid and early detection of aquatic plant invasive species, but currently suffers from substantial unknowns that limit its widespread use in monitoring programs. We conducted the first study to test the factors related to eDNA-based detectability of 2 invasive aquatic plants, Egeria densa and Myriophyllum spicatum, over extended period
Authors
Lauren M Kuehne, Carl Ostberg, Dorothy M. Chase, Jeffrey J. Duda, Julian D. Olden
Development of a quantitative PCR assay for detecting Egeria densa in environmental DNA samples
Brazilian elodea (Egeria densa) is an invasive freshwater plant that demonstrates widespread ecological impacts in freshwater ecosystems and causes substantial economic damage. Here, we developed an environmental DNA assay for detection of E. densa to provide resource managers with a tool for early detection, identification, and monitoring of invasive populations.
Authors
Dorothy M. Chase, Lauren M Kuehne, Julian D. Olden, Carl Ostberg
Evaluation of environmental DNA surveys for identifying occupancy and spatial distribution of Pacific Lamprey (Entosphenus tridentatus) and Lampetra spp. in a Washington coast watershed
Surveys of environmental DNA (eDNA) have become an important and multifaceted tool for monitoring and identifying distributions and occupancy of aquatic species. This tool is attractive because it is powerful, easy to apply, and provides an alternative to traditional field survey methods. However, validating eDNA survey methods against traditional field survey methods is warranted prior to their a
Authors
Carl Ostberg, Dorothy Murphy Chase, Marshal Hoy, Jeff Duda, Michael Hayes, Jeffrey Jolley, Gregory S Silver, Carrie Cook-Tabor
Distribution and seasonal differences in Pacific Lamprey and Lampetra spp eDNA across 18 Puget Sound watersheds
Lampreys have a worldwide distribution, are functionally important to ecological communities and serve significant roles in many cultures. In Pacific coast drainages of North America, lamprey populations have suffered large declines. However, lamprey population status and trends within many areas of this region are unknown and such information is needed for advancing conservation goals. We develop
Authors
Carl O. Ostberg, Dorothy M. Chase, Michael C. Hayes, Jeffrey J. Duda
Hybridization between Yellowstone cutthroat trout and rainbow trout alters the expression of muscle growth-related genes and their relationships with growth patterns
Hybridization creates novel gene combinations that may generate important evolutionary novelty, but may also reduce existing adaptation by interrupting inherent biological processes, such as genotype-environment interactions. Hybridization often causes substantial change in patterns of gene expression, which, in turn, may cause phenotypic change. Rainbow trout (Oncorhynchus mykiss) and cutthroat t
Authors
Carl O. Ostberg, Dorothy M. Chase, Lorenz Hauser
Comparison of the membrane-filtration fluorescent antibody test, the enzyme-linked immunosorbent assay, and the polymerase chain reaction to detect Renibacterium salmoninarum in salmon ovarian fluid
Ovarian fluid samples from naturally infected chinook salmon (Oncorhynchus tshawytscha) were examined for the presence of Renibacterium salmoninarum by the membrane-filtration fluorescent antibody test (MF-FAT), an antigen capture enzyme-linked immunosorbent assay (ELISA), and a nested polymerase chain reaction (PCR). On the basis of the MF-FAT, 64% (66/103) samples contained detectable levels of
Authors
Ronald J. Pascho, Dorothy M. Chase, Constance L. McKibben
Testing of candidate non-lethal sampling methods for detection of Renibacterium salmoninarum in juvenile Chinook salmon Oncorhynchus tshawytscha
Non-lethal pathogen testing can be a useful tool for fish disease research and management. Our research objectives were to determine if (1) fin clips, gill snips, surface mucus scrapings, blood draws, or kidney biopsies could be obtained non-lethally from 3 to 15 g Chinook salmon Oncorhynchus tshawytscha, (2) non-lethal samples could accurately discriminate between fish exposed to the bacterial ki
Authors
Diane G. Elliott, Constance L. McKibben, Carla M. Conway, Maureen K. Purcell, Dorothy M. Chase, Lynn M. Applegate
Distribution of Pacific lamprey Entosphenus tridentatus in watersheds of Puget Sound Based on smolt monitoring data
Lamprey populations are in decline worldwide and the status of Pacific lamprey (Entosphenus tridentatus) is a topic of current interest. They and other lamprey species cycle nutrients and serve as prey in riverine ecosystems. To determine the current distribution of Pacific lamprey in major watersheds flowing into Puget Sound, Washington, we sampled lamprey captured during salmonid smolt monitorin
Authors
Michael C. Hayes, Richard Hays, Stephen P. Rubin, Dorothy M. Chase, Molly Hallock, Carrie Cook-Tabor, Christina W. Luzier, Mary L. Moser
Temporal genetic monitoring of hybridization between native westslope cutthroat trout and introduced rainbow trout in the Stehekin River, Washington
Introgressive hybridization with introduced rainbow trout (RBT) (Oncorhynchus mykiss) has led to the loss of native cutthroat trout species (O. clarkii) throughout their range, creating conservation concerns. Monitoring temporal hybridization trends provides resource managers with a tool for determining population status and information for establishing conservation goals for native cutthroat trou
Authors
Carl O. Ostberg, Dorothy M. Chase
Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis
Renibacterium salmoninarum is an important salmonid pathogen that is difficult to culture. We developed and assessed a real-time, quantitative, polymerase chain reaction (qPCR) assay for the detection and enumeration of R. salmoninarum. The qPCR is based on TaqMan technology and amplifies a 69-base pair (bp) region of the gene encoding the major soluble antigen (MSA) of R. salmoninarum. The qPCR a
Authors
D.M. Chase, D.G. Elliott, R.J. Pascho
Science and Products
- Data
Environmental DNA (eDNA) is an Effective Tool to Track Recolonizing Migratory Fish Following Large-Scale Dam Removal, field data
We collected environmental DNA (eDNA) data from the Elwha River, home to the world?s largest dam removal project, to track the spatial and temporal patterns of species responses following dam removal. In total, we collected data for 11 different fish taxa, sampled at 25 sites ranging across 56 river kilometers in a wilderness river for 4 years following dam removal. We show that eDNA can effectiveDetection of invasive aquatic plants Myriophyllum spicatum and Egeria densa in lakes using eDNA, field and mesocosm data
We conducted a study to test the factors related to detectability of two invasive aquatic plants (Egeria densa and Myriophyllym spicatum) using environmental DNA (eDNA), over extended periods of time, and specifically examined how plant growth stage and abundance relates to eDNA detection in semi-natural and natural conditions. This dataset is from sampling performed in summer of 2018 in lakes witDistribution and seasonal differences in Pacific Lamprey and Lampetra spp eDNA across 18 Puget Sound watersheds, 2014 and 2015
These data contain all the raw results needed to support the conclusions for the final product. These data are water sampling locations (latitude and longitude), date of water sampling, quantitative PCR values for each water sample, and stream flow at USGS stream gauging stations on sampling day. - Publications
Filter Total Items: 15
Ontogeny of eDNA shedding during early development in Chinook Salmon (Oncorhynchus tshawytscha)
Knowledge of the timing of major life history events in aquatic species is important for informing conservation and resource management planning. Accordingly, surveys of environmental DNA (eDNA) have been performed to determine the efficacy of eDNA for providing information on life history events, primarily focusing on the timing of events associated with spawning, and these studies have proved suAuthorsCarl Ostberg, Dorothy M. ChaseEnvironmental DNA is an effective tool to track recolonizing migratory fish following large‐scale dam removal
Environmental DNA (eDNA) has emerged as a potentially powerful tool for use in conservation and resource management, including for tracking the recolonization dynamics of fish populations. We used eDNA to assess the effectiveness of dam removal to restore fish passage on the Elwha River in Washington State (USA). Using a suite of 11 species‐specific eDNA polymerase chain reaction (PCR) assays, weAuthorsJeffrey J. Duda, Marshal Hoy, Dorothy M. Chase, George R. Pess, Samuel J. Brenkman, Michael M McHenry, Carl OstbergUse of environmental DNA to detect the invasive aquatic plants Myriophyllum spicatum and Egeria densa in lakes
Environmental DNA (eDNA) analysis offers a promising tool for rapid and early detection of aquatic plant invasive species, but currently suffers from substantial unknowns that limit its widespread use in monitoring programs. We conducted the first study to test the factors related to eDNA-based detectability of 2 invasive aquatic plants, Egeria densa and Myriophyllum spicatum, over extended periodAuthorsLauren M Kuehne, Carl Ostberg, Dorothy M. Chase, Jeffrey J. Duda, Julian D. OldenDevelopment of a quantitative PCR assay for detecting Egeria densa in environmental DNA samples
Brazilian elodea (Egeria densa) is an invasive freshwater plant that demonstrates widespread ecological impacts in freshwater ecosystems and causes substantial economic damage. Here, we developed an environmental DNA assay for detection of E. densa to provide resource managers with a tool for early detection, identification, and monitoring of invasive populations.AuthorsDorothy M. Chase, Lauren M Kuehne, Julian D. Olden, Carl OstbergEvaluation of environmental DNA surveys for identifying occupancy and spatial distribution of Pacific Lamprey (Entosphenus tridentatus) and Lampetra spp. in a Washington coast watershed
Surveys of environmental DNA (eDNA) have become an important and multifaceted tool for monitoring and identifying distributions and occupancy of aquatic species. This tool is attractive because it is powerful, easy to apply, and provides an alternative to traditional field survey methods. However, validating eDNA survey methods against traditional field survey methods is warranted prior to their aAuthorsCarl Ostberg, Dorothy Murphy Chase, Marshal Hoy, Jeff Duda, Michael Hayes, Jeffrey Jolley, Gregory S Silver, Carrie Cook-TaborDistribution and seasonal differences in Pacific Lamprey and Lampetra spp eDNA across 18 Puget Sound watersheds
Lampreys have a worldwide distribution, are functionally important to ecological communities and serve significant roles in many cultures. In Pacific coast drainages of North America, lamprey populations have suffered large declines. However, lamprey population status and trends within many areas of this region are unknown and such information is needed for advancing conservation goals. We developAuthorsCarl O. Ostberg, Dorothy M. Chase, Michael C. Hayes, Jeffrey J. DudaHybridization between Yellowstone cutthroat trout and rainbow trout alters the expression of muscle growth-related genes and their relationships with growth patterns
Hybridization creates novel gene combinations that may generate important evolutionary novelty, but may also reduce existing adaptation by interrupting inherent biological processes, such as genotype-environment interactions. Hybridization often causes substantial change in patterns of gene expression, which, in turn, may cause phenotypic change. Rainbow trout (Oncorhynchus mykiss) and cutthroat tAuthorsCarl O. Ostberg, Dorothy M. Chase, Lorenz HauserComparison of the membrane-filtration fluorescent antibody test, the enzyme-linked immunosorbent assay, and the polymerase chain reaction to detect Renibacterium salmoninarum in salmon ovarian fluid
Ovarian fluid samples from naturally infected chinook salmon (Oncorhynchus tshawytscha) were examined for the presence of Renibacterium salmoninarum by the membrane-filtration fluorescent antibody test (MF-FAT), an antigen capture enzyme-linked immunosorbent assay (ELISA), and a nested polymerase chain reaction (PCR). On the basis of the MF-FAT, 64% (66/103) samples contained detectable levels ofAuthorsRonald J. Pascho, Dorothy M. Chase, Constance L. McKibbenTesting of candidate non-lethal sampling methods for detection of Renibacterium salmoninarum in juvenile Chinook salmon Oncorhynchus tshawytscha
Non-lethal pathogen testing can be a useful tool for fish disease research and management. Our research objectives were to determine if (1) fin clips, gill snips, surface mucus scrapings, blood draws, or kidney biopsies could be obtained non-lethally from 3 to 15 g Chinook salmon Oncorhynchus tshawytscha, (2) non-lethal samples could accurately discriminate between fish exposed to the bacterial kiAuthorsDiane G. Elliott, Constance L. McKibben, Carla M. Conway, Maureen K. Purcell, Dorothy M. Chase, Lynn M. ApplegateDistribution of Pacific lamprey Entosphenus tridentatus in watersheds of Puget Sound Based on smolt monitoring data
Lamprey populations are in decline worldwide and the status of Pacific lamprey (Entosphenus tridentatus) is a topic of current interest. They and other lamprey species cycle nutrients and serve as prey in riverine ecosystems. To determine the current distribution of Pacific lamprey in major watersheds flowing into Puget Sound, Washington, we sampled lamprey captured during salmonid smolt monitorinAuthorsMichael C. Hayes, Richard Hays, Stephen P. Rubin, Dorothy M. Chase, Molly Hallock, Carrie Cook-Tabor, Christina W. Luzier, Mary L. MoserTemporal genetic monitoring of hybridization between native westslope cutthroat trout and introduced rainbow trout in the Stehekin River, Washington
Introgressive hybridization with introduced rainbow trout (RBT) (Oncorhynchus mykiss) has led to the loss of native cutthroat trout species (O. clarkii) throughout their range, creating conservation concerns. Monitoring temporal hybridization trends provides resource managers with a tool for determining population status and information for establishing conservation goals for native cutthroat trouAuthorsCarl O. Ostberg, Dorothy M. ChaseDetection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative polymerase chain reaction analysis
Renibacterium salmoninarum is an important salmonid pathogen that is difficult to culture. We developed and assessed a real-time, quantitative, polymerase chain reaction (qPCR) assay for the detection and enumeration of R. salmoninarum. The qPCR is based on TaqMan technology and amplifies a 69-base pair (bp) region of the gene encoding the major soluble antigen (MSA) of R. salmoninarum. The qPCR aAuthorsD.M. Chase, D.G. Elliott, R.J. Pascho