Jason Ferrante , Ph.D. (Former Employee)
Science and Products
Filter Total Items: 16
A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
Objective We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration...
Authors
Jason A. Ferrante, Michelle R. Giles, Emily Benzie, Margaret Hunter
Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in Vulnerable manatee populations Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in Vulnerable manatee populations
Environmental DNA (eDNA) detection is a technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees. For eDNA measurement, genetic material shed into the environment is concentrated from water samples and analyzed for the presence of target species. Cytochrome bquantitative PCR and droplet digital PCR eDNA assays...
Authors
Margaret Hunter, Gaia Meigs-Friend, Jason A. Ferrante, Aristide Takoukam Kamla, Robert Dorazio, Lucy Keith Diagne, Fabia Luna, Janet M. Lanyon, James P. Reid
Development and validation of quantitative PCR assays to measure cytokine transcript levels in the Florida manatee (Trichechus manatus latirostris) Development and validation of quantitative PCR assays to measure cytokine transcript levels in the Florida manatee (Trichechus manatus latirostris)
Cytokines have important roles in the mammalian response to viral and bacterial infections, trauma, and wound healing. Because of early cytokine production after physiologic stresses, the regulation of messenger RNA (mRNA) transcripts can be used to assess immunologic responses before changes in protein production. To detect and assess early immune changes in endangered Florida manatees
Authors
Jason A. Ferrante, Margaret Hunter, James F.X. Wellehan
Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA
A set of universal guidelines is needed to determine the limit of detection (LOD) in PCR-based analyses of low concentration DNA. In particular, environmental DNA (eDNA) studies require sensitive and reliable methods to detect rare and cryptic species through shed genetic material in environmental samples. Current strategies for assessing detection limits of eDNA are either too stringent...
Authors
Margaret Hunter, Robert M. Dorazio, John S. Butterfield, Gaia Meigs-Friend, Leo Nico, Jason A. Ferrante
Science and Products
Filter Total Items: 16
A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation A novel technique for isolating DNA from Tempus™ blood RNA tubes after RNA isolation
Objective We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe a novel protocol to additionally isolate high-quality DNA from the supernatant remaining after the RNA isolation methodology. Metrics used to qualify DNA quality included measuring the concentration...
Authors
Jason A. Ferrante, Michelle R. Giles, Emily Benzie, Margaret Hunter
Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in Vulnerable manatee populations Surveys of environmental DNA (eDNA): a new approach to estimate occurrence in Vulnerable manatee populations
Environmental DNA (eDNA) detection is a technique used to non-invasively detect cryptic, low density, or logistically difficult-to-study species, such as imperiled manatees. For eDNA measurement, genetic material shed into the environment is concentrated from water samples and analyzed for the presence of target species. Cytochrome bquantitative PCR and droplet digital PCR eDNA assays...
Authors
Margaret Hunter, Gaia Meigs-Friend, Jason A. Ferrante, Aristide Takoukam Kamla, Robert Dorazio, Lucy Keith Diagne, Fabia Luna, Janet M. Lanyon, James P. Reid
Development and validation of quantitative PCR assays to measure cytokine transcript levels in the Florida manatee (Trichechus manatus latirostris) Development and validation of quantitative PCR assays to measure cytokine transcript levels in the Florida manatee (Trichechus manatus latirostris)
Cytokines have important roles in the mammalian response to viral and bacterial infections, trauma, and wound healing. Because of early cytokine production after physiologic stresses, the regulation of messenger RNA (mRNA) transcripts can be used to assess immunologic responses before changes in protein production. To detect and assess early immune changes in endangered Florida manatees
Authors
Jason A. Ferrante, Margaret Hunter, James F.X. Wellehan
Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA Detection limits of quantitative and digital PCR assays and their influence in presence-absence surveys of environmental DNA
A set of universal guidelines is needed to determine the limit of detection (LOD) in PCR-based analyses of low concentration DNA. In particular, environmental DNA (eDNA) studies require sensitive and reliable methods to detect rare and cryptic species through shed genetic material in environmental samples. Current strategies for assessing detection limits of eDNA are either too stringent...
Authors
Margaret Hunter, Robert M. Dorazio, John S. Butterfield, Gaia Meigs-Friend, Leo Nico, Jason A. Ferrante