Laurence G Miller (Former Employee)
Science and Products
Filter Total Items: 55
Large carbon isotope fractionation associated with oxidation of methyl halides by methylotrophic bacteria Large carbon isotope fractionation associated with oxidation of methyl halides by methylotrophic bacteria
The largest biological fractionations of stable carbon isotopes observed in nature occur during production of methane by methanogenic archaea. These fractionations result in substantial (as much as ≈70‰) shifts in δ13C relative to the initial substrate. We now report that a stable carbon isotopic fractionation of comparable magnitude (up to 70‰) occurs during oxidation of methyl halides...
Authors
L.G. Miller, Robert M. Kalin, S.E. McCauley, John T.G. Hamilton, D.B. Harper, D.B. Millet, R.S. Oremland, Allen H. Goldstein
Bacterial dissimilatory reduction of arsenate and sulfate in meromictic Mono Lake, California Bacterial dissimilatory reduction of arsenate and sulfate in meromictic Mono Lake, California
The stratified (meromictic) water column of alkaline and hypersaline Mono Lake, California, contains high concentrations of dissolved inorganic arsenic (~200 ??mol/L). Arsenic speciation changes from arsenate [As (V)] to arsenite [As (III)] with the transition from oxic surface waters (misolimnion) to anoxic bottom waters (monimolimnion). A radioassay was devised to measure the reduction...
Authors
R.S. Oremland, P.R. Dowdle, S. Hoeft, J.O. Sharp, J.K. Schaefer, L.G. Miller, Blum J. Switzer, R. L. Smith, N.S. Bloom, D. Wallschlaeger
Oxidation of ammonia and methane in an alkaline, saline lake Oxidation of ammonia and methane in an alkaline, saline lake
The oxidation of ammonia (NH3) and methane (CH4) was investigated in an alkaline saline lake, Mono Lake, California (U.S.A.). Ammonia oxidation was examined in April and July 1995 by comparing dark 14CO2 fixation rates in the presence or absence of methyl fluoride (MeF), an inhibitor of NH3 oxidation. Ammonia oxidizer‐mediated dark 14CO2fixation rates were similar in surface (5–7 m) and...
Authors
S.B. Joye, T.L. Connell, L.G. Miller, R.S. Oremland, R.S. Jellison
Difluoromethane, a new and improved inhibitor of methanotrophy Difluoromethane, a new and improved inhibitor of methanotrophy
Difluoromethane (HFC-32; DFM) is compared to acetylene and methyl fluoride as an inhibitor of methanotrophy in cultures and soils. DFM was found to be a reversible inhibitor of CH4 oxidation byMethylococcus capsulatus (Bath). Consumption of CH4 in soil was blocked by additions of low levels of DFM (0.03 kPa), and this inhibition was reversed by DFM removal. Although a small quantity of...
Authors
L.G. Miller, C. Sasson, R.S. Oremland
Bacterial oxidation of methyl bromide in Mono Lake, California Bacterial oxidation of methyl bromide in Mono Lake, California
The oxidation of methyl bromide (MeBr) in the water column of Mono Lake, CA, was studied by measuring the formation of H14CO3 from [14C]MeBr. Potential oxidation was detected throughout the water column, with highest rates occurring in the epilimnion (5-12 m depth). The oxidation of MeBr was eliminated by filter-sterilization, thereby demonstrating the involvement of bacteria. Vertical...
Authors
T.L. Connell, S.B. Joye, L.G. Miller, R.S. Oremland
Bacterial oxidation of methyl bromide in fumigated agricultural soils Bacterial oxidation of methyl bromide in fumigated agricultural soils
The oxidation of [14C]methyl bromide ([14C]MeBr) to 14CO2 was measured in field experiments with soils collected from two strawberry plots fumigated with mixtures of MeBr and chloropicrin (CCl3NO2). Although these fumigants are considered potent biocides, we found that the highest rates of MeBr oxidation occurred 1 to 2 days after injection when the fields were tarped, rather than before...
Authors
L.G. Miller, T.L. Connell, J.R. Guidetti, R.S. Oremland
Science and Products
Filter Total Items: 55
Large carbon isotope fractionation associated with oxidation of methyl halides by methylotrophic bacteria Large carbon isotope fractionation associated with oxidation of methyl halides by methylotrophic bacteria
The largest biological fractionations of stable carbon isotopes observed in nature occur during production of methane by methanogenic archaea. These fractionations result in substantial (as much as ≈70‰) shifts in δ13C relative to the initial substrate. We now report that a stable carbon isotopic fractionation of comparable magnitude (up to 70‰) occurs during oxidation of methyl halides...
Authors
L.G. Miller, Robert M. Kalin, S.E. McCauley, John T.G. Hamilton, D.B. Harper, D.B. Millet, R.S. Oremland, Allen H. Goldstein
Bacterial dissimilatory reduction of arsenate and sulfate in meromictic Mono Lake, California Bacterial dissimilatory reduction of arsenate and sulfate in meromictic Mono Lake, California
The stratified (meromictic) water column of alkaline and hypersaline Mono Lake, California, contains high concentrations of dissolved inorganic arsenic (~200 ??mol/L). Arsenic speciation changes from arsenate [As (V)] to arsenite [As (III)] with the transition from oxic surface waters (misolimnion) to anoxic bottom waters (monimolimnion). A radioassay was devised to measure the reduction...
Authors
R.S. Oremland, P.R. Dowdle, S. Hoeft, J.O. Sharp, J.K. Schaefer, L.G. Miller, Blum J. Switzer, R. L. Smith, N.S. Bloom, D. Wallschlaeger
Oxidation of ammonia and methane in an alkaline, saline lake Oxidation of ammonia and methane in an alkaline, saline lake
The oxidation of ammonia (NH3) and methane (CH4) was investigated in an alkaline saline lake, Mono Lake, California (U.S.A.). Ammonia oxidation was examined in April and July 1995 by comparing dark 14CO2 fixation rates in the presence or absence of methyl fluoride (MeF), an inhibitor of NH3 oxidation. Ammonia oxidizer‐mediated dark 14CO2fixation rates were similar in surface (5–7 m) and...
Authors
S.B. Joye, T.L. Connell, L.G. Miller, R.S. Oremland, R.S. Jellison
Difluoromethane, a new and improved inhibitor of methanotrophy Difluoromethane, a new and improved inhibitor of methanotrophy
Difluoromethane (HFC-32; DFM) is compared to acetylene and methyl fluoride as an inhibitor of methanotrophy in cultures and soils. DFM was found to be a reversible inhibitor of CH4 oxidation byMethylococcus capsulatus (Bath). Consumption of CH4 in soil was blocked by additions of low levels of DFM (0.03 kPa), and this inhibition was reversed by DFM removal. Although a small quantity of...
Authors
L.G. Miller, C. Sasson, R.S. Oremland
Bacterial oxidation of methyl bromide in Mono Lake, California Bacterial oxidation of methyl bromide in Mono Lake, California
The oxidation of methyl bromide (MeBr) in the water column of Mono Lake, CA, was studied by measuring the formation of H14CO3 from [14C]MeBr. Potential oxidation was detected throughout the water column, with highest rates occurring in the epilimnion (5-12 m depth). The oxidation of MeBr was eliminated by filter-sterilization, thereby demonstrating the involvement of bacteria. Vertical...
Authors
T.L. Connell, S.B. Joye, L.G. Miller, R.S. Oremland
Bacterial oxidation of methyl bromide in fumigated agricultural soils Bacterial oxidation of methyl bromide in fumigated agricultural soils
The oxidation of [14C]methyl bromide ([14C]MeBr) to 14CO2 was measured in field experiments with soils collected from two strawberry plots fumigated with mixtures of MeBr and chloropicrin (CCl3NO2). Although these fumigants are considered potent biocides, we found that the highest rates of MeBr oxidation occurred 1 to 2 days after injection when the fields were tarped, rather than before...
Authors
L.G. Miller, T.L. Connell, J.R. Guidetti, R.S. Oremland