Luke R Iwanowicz, Ph.D. (Former Employee)
Science and Products
Filter Total Items: 27
No Result Found
Filter Total Items: 110
Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens
Estrogens are recognized as modulators of immune responses in mammals and teleosts. While it is known that the effects of estrogens are mediated via leukocyte-specific estrogen receptors (ERs) in humans and mice, leucocyte-specific estrogen receptor expression and the effects of estrogens on this cell population is less explored and poorly understood in teleosts. Here in, we verify that...
Authors
Luke R. Iwanowicz, James L. Stafford, Reynaldo Patiño, Eva Bengten, Norman W. Miller, Vicki Blazer
In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush) In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)
In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing...
Authors
Christopher A. Ottinger, Dale C. Honeyfield, Christine L. Densmore, Luke R. Iwanowicz
Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results
During fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse...
Authors
Vicki Blazer, Patricia M. Mazik, Luke R. Iwanowicz, Ryan Braham, Cassidy Hahn, Heather L. Walsh, Adam Sperry
Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples
Background: Environmental endocrine disruptors (EED) are exogenous chemicals that mimic endogenous hormones, such as estrogens. Previous studies using a zebrafish transgenic reporter demonstrated that the EEDs bisphenol A and genistein preferentially activate estrogen receptors (ER) in the larval heart compared to the liver. However, it was not known whether the transgenic zebrafish...
Authors
Daniel A. Gorelick, Luke R. Iwanowicz, Alice L. Hung, Vicki Blazer, Marnie E. Halpern
Biological effects-based tools for monitoring impacted surface waters in the Great Lakes: a multiagency program in support of the Great Lakes Restoration Initiative Biological effects-based tools for monitoring impacted surface waters in the Great Lakes: a multiagency program in support of the Great Lakes Restoration Initiative
There is increasing demand for the implementation of effects-based monitoring and surveillance (EBMS) approaches in the Great Lakes Basin to complement traditional chemical monitoring. Herein, we describe an ongoing multiagency effort to develop and implement EBMS tools, particularly with regard to monitoring potentially toxic chemicals and assessing Areas of Concern (AOCs), as...
Authors
Drew R. Ekman, Gerald T. Ankley, Vicki Blazer, Timothy W. Collette, Natàlia Garcia-Reyero, Luke R. Iwanowicz, Zachary G. Jorgensen, Kathy Lee, Pat M. Mazik, David H. Miller, Edward J. Perkins, Edwin T. Smith, Joseph E. Tietge, Daniel L. Villeneuve
Identification of largemouth bass virus in the introduced Northern snakehead inhabiting the Cheasapeake Bay watershed Identification of largemouth bass virus in the introduced Northern snakehead inhabiting the Cheasapeake Bay watershed
The Northern Snakehead Channa argus is an introduced species that now inhabits the Chesapeake Bay. During a preliminary survey for introduced pathogens possibly harbored by these fish in Virginia waters, a filterable agent was isolated from five specimens that produced cytopathic effects in BF-2 cells. Based on PCR amplification and partial sequencing of the major capsid protein (MCP)...
Authors
Luke R. Iwanowicz, Christine L. Densmore, Cassidy M. Hahn, Phillip McAllister, John Odenkirk
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 27
No Result Found
Filter Total Items: 110
Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens Channel catfish (Ictalurus punctatus) leukocytes express estrogen receptor isoforms ERα and ERβ2 and are functionally modulated by estrogens
Estrogens are recognized as modulators of immune responses in mammals and teleosts. While it is known that the effects of estrogens are mediated via leukocyte-specific estrogen receptors (ERs) in humans and mice, leucocyte-specific estrogen receptor expression and the effects of estrogens on this cell population is less explored and poorly understood in teleosts. Here in, we verify that...
Authors
Luke R. Iwanowicz, James L. Stafford, Reynaldo Patiño, Eva Bengten, Norman W. Miller, Vicki Blazer
In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush) In vitro immune functions in thiamine-replete and -depleted lake trout (Salvelinus namaycush)
In this study we examined the impacts of in vivo thiamine deficiency on lake trout leukocyte function measured in vitro. When compared outside the context of individual-specific thiamine concentrations no significant differences were observed in leukocyte bactericidal activity or in concanavalin A (Con A), and phytohemagglutinin-P (PHA-P) stimulated leukocyte proliferation. Placing...
Authors
Christopher A. Ottinger, Dale C. Honeyfield, Christine L. Densmore, Luke R. Iwanowicz
Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results
During fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse...
Authors
Vicki Blazer, Patricia M. Mazik, Luke R. Iwanowicz, Ryan Braham, Cassidy Hahn, Heather L. Walsh, Adam Sperry
Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples Transgenic zebrafish reveal tissue-specific differences in estrogen signaling in response to environmental water samples
Background: Environmental endocrine disruptors (EED) are exogenous chemicals that mimic endogenous hormones, such as estrogens. Previous studies using a zebrafish transgenic reporter demonstrated that the EEDs bisphenol A and genistein preferentially activate estrogen receptors (ER) in the larval heart compared to the liver. However, it was not known whether the transgenic zebrafish...
Authors
Daniel A. Gorelick, Luke R. Iwanowicz, Alice L. Hung, Vicki Blazer, Marnie E. Halpern
Biological effects-based tools for monitoring impacted surface waters in the Great Lakes: a multiagency program in support of the Great Lakes Restoration Initiative Biological effects-based tools for monitoring impacted surface waters in the Great Lakes: a multiagency program in support of the Great Lakes Restoration Initiative
There is increasing demand for the implementation of effects-based monitoring and surveillance (EBMS) approaches in the Great Lakes Basin to complement traditional chemical monitoring. Herein, we describe an ongoing multiagency effort to develop and implement EBMS tools, particularly with regard to monitoring potentially toxic chemicals and assessing Areas of Concern (AOCs), as...
Authors
Drew R. Ekman, Gerald T. Ankley, Vicki Blazer, Timothy W. Collette, Natàlia Garcia-Reyero, Luke R. Iwanowicz, Zachary G. Jorgensen, Kathy Lee, Pat M. Mazik, David H. Miller, Edward J. Perkins, Edwin T. Smith, Joseph E. Tietge, Daniel L. Villeneuve
Identification of largemouth bass virus in the introduced Northern snakehead inhabiting the Cheasapeake Bay watershed Identification of largemouth bass virus in the introduced Northern snakehead inhabiting the Cheasapeake Bay watershed
The Northern Snakehead Channa argus is an introduced species that now inhabits the Chesapeake Bay. During a preliminary survey for introduced pathogens possibly harbored by these fish in Virginia waters, a filterable agent was isolated from five specimens that produced cytopathic effects in BF-2 cells. Based on PCR amplification and partial sequencing of the major capsid protein (MCP)...
Authors
Luke R. Iwanowicz, Christine L. Densmore, Cassidy M. Hahn, Phillip McAllister, John Odenkirk
Non-USGS Publications**
Iwanowicz, L., L Brown, B Eltz, F Juanes and J Murt (2004) Book Review. In a Perfect Ocean: The state of fisheries and ecosystems in the North Atlantic Ocean. Reviews in Fish Biology and Fisheries.14: 149-151.
McIntire, M., L R Iwanowicz and A E Goodwin (2003) Molecular, physical and clinical evidence that Golden Shiner Virus (GSV) and Grass Carp Reovirus (GCRV) are variants of the same virus. Journal of Aquatic Animal Health. 15: 257-263.
Iwanowicz, L.R. and A E Goodwin (2002) Characterization of a new bacilliform fathead minnow rhabdovirus that produces syncytia in tissue culture. Archives of Virology 147: 889 – 916.
Iwanowicz, L.R. A E Goodwin and J Harshbarger (2001) Embryonal rhabdomyosarcoma of the giant gourami, Colisa fasciata (Bloch & Schneider). Journal of Fish Diseases 24:177-179
Iwanowicz, L.R. A E Goodwin and N Heil (2000) A small RNA virus isolated from apparently healthy wild sandbar shiners, Notropis scepticus. Journal of Fish Diseases 23: 349-352.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government