Maureen K Purcell, Ph.D. (Former Employee)
Science and Products
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Development of a real-time PCR assay for detection of planktonic red king crab (Paralithodes camtschaticus (Tilesius 1815)) larvae Development of a real-time PCR assay for detection of planktonic red king crab (Paralithodes camtschaticus (Tilesius 1815)) larvae
The Alaskan red king crab (Paralithodes camtschaticus) fishery was once one of the most economically important single-species fisheries in the world, but is currently depressed. This fishery would benefit from improved stock assessment capabilities. Larval crab distribution is patchy temporally and spatially, requiring extensive sampling efforts to locate and track larval dispersal...
Authors
Pamela C. Jensen, Maureen K. Purcell, J. Frank Morado, Ginny L. Eckert
Restricted growth of U-type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity Restricted growth of U-type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity
Previously, we demonstrated that a representative M genogroup type strain of infectious haematopoietic necrosis virus (IHNV) from rainbow trout grows well in rainbow trout‐derived RTG‐2 cells, but a U genogroup type strain from sockeye salmon has restricted growth, associated with reduced genome replication and mRNA transcription. Here, we analysed further the mechanisms for this growth
Authors
J. W. Park, C. H. Moon, A. Harmache, A. R. Wargo, M. K. Purcell, M. Bremont, Gael Kurath
Host specificity and ecology of infectious hematopoietic necrosis virus (IHNV) in Pacific salmonids Host specificity and ecology of infectious hematopoietic necrosis virus (IHNV) in Pacific salmonids
Some circumstances IHNV infection can cause acute disease with mortality ranging from 5-90% in host populations. Genetic typing of IHNV field isolates has shown that three major genetic groups of the virus occur in North America. These groups are designated the U, M, and L virus genogroups because they occur in the upper, middle, and lower portions of the geographic range of IHNV in...
Authors
Gael Kurath, A. K. Garver, M. K. Purcell, Ma. Penaranda, Svetlana Rudakova
Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting
Real-time, or quantitative, polymerase chain reaction (qPCR) is quickly supplanting other molecular methods for detecting the nucleic acids of human and other animal pathogens owing to the speed and robustness of the technology. As the aquatic animal health community moves toward implementing national diagnostic testing schemes, it will need to evaluate how qPCR technology should be...
Authors
Maureen K. Purcell, Rodman G. Getchell, Carol A. McClure, S.E. Weber, Kyle A. Garver
Comparative evaluation of molecular diagnostic tests for Nucleospora salmonis and prevalence in migrating juvenile salmonids from the Snake River, USA Comparative evaluation of molecular diagnostic tests for Nucleospora salmonis and prevalence in migrating juvenile salmonids from the Snake River, USA
Nucleospora salmonis is an intranuclear microsporidian that primarily infects lymphoblast cells and contributes to chronic lymphoblastosis and a leukemia-like condition in a range of salmonid species. The primary goal of this study was to evaluate the prevalence of N. salmonis in out-migrating juvenile hatchery and wild Chinook salmon Oncorhynchus tshawytscha and steelhead O. mykiss from...
Authors
Samantha Badil, Diane G. Elliott, Tomofumi Kurobe, Ronald P. Hedrick, Kathy Clemens, Marilyn Blair, Maureen K. Purcell
Sequence analysis of the internal transcribed spacer (ITS) region reveals a novel clade of Ichthyophonus sp. from rainbow trout Sequence analysis of the internal transcribed spacer (ITS) region reveals a novel clade of Ichthyophonus sp. from rainbow trout
The mesomycetozoean parasite Ichthyophonus hoferi is most commonly associated with marine fish hosts but also occurs in some components of the freshwater rainbow trout Oncorhynchus mykiss aquaculture industry in Idaho, USA. It is not certain how the parasite was introduced into rainbow trout culture, but it might have been associated with the historical practice of feeding raw, ground...
Authors
C. Rasmussen, M. K. Purcell, J.L. Gregg, S. E. LaPatra, J. R. Winton, P.K. Hershberger
Non-USGS Publications**
Purcell, M., G. Mackey, E. LaHood, H. Huber, and L. Park. 2004. Molecular methods for the genetic identification of salmonid prey from Pacific harbor seal (Phoca vitulina richardsi ) scat. Fish. Bull. 1002: 213-220.
Lage, C., M. Purcell, M. Forgarty, and I. Kornfield. 2001. Microsatellite evaluation of haddock (Melanogrammus aeglefinus) stocks in the northwest Atlantic Ocean. Can. J. Fish. Aquat. Sci. 58: 982-990. DOI: 10.1139/cjfas-58-5-982.
Purcell, M.K., J-L. Mu, D.C. Higgins, R. Elango, H. Whitmore, S. Harris, and B. Paigen. 2001. Fine mapping of Ath6, a quantitative trait locus for atherosclerosis in mice. Mammalian Genome 12(7): 495-500. DOI: 10.1007/s00335001-0006-9.
Purcell, M.K., I.L. Kornfield, M.J. Fogarty and A. Parker. (1996) Interdecadal heterogeneity of mitochondrial DNA in Atlantic haddock (Melanogrammus aeglefinus) from Georges Bank. Molecular Marine Biology and Biotechnology. 5:185-192.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
Science and Products
Filter Total Items: 14
Filter Total Items: 88
Development of a real-time PCR assay for detection of planktonic red king crab (Paralithodes camtschaticus (Tilesius 1815)) larvae Development of a real-time PCR assay for detection of planktonic red king crab (Paralithodes camtschaticus (Tilesius 1815)) larvae
The Alaskan red king crab (Paralithodes camtschaticus) fishery was once one of the most economically important single-species fisheries in the world, but is currently depressed. This fishery would benefit from improved stock assessment capabilities. Larval crab distribution is patchy temporally and spatially, requiring extensive sampling efforts to locate and track larval dispersal...
Authors
Pamela C. Jensen, Maureen K. Purcell, J. Frank Morado, Ginny L. Eckert
Restricted growth of U-type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity Restricted growth of U-type infectious haematopoietic necrosis virus (IHNV) in rainbow trout cells may be linked to casein kinase II activity
Previously, we demonstrated that a representative M genogroup type strain of infectious haematopoietic necrosis virus (IHNV) from rainbow trout grows well in rainbow trout‐derived RTG‐2 cells, but a U genogroup type strain from sockeye salmon has restricted growth, associated with reduced genome replication and mRNA transcription. Here, we analysed further the mechanisms for this growth
Authors
J. W. Park, C. H. Moon, A. Harmache, A. R. Wargo, M. K. Purcell, M. Bremont, Gael Kurath
Host specificity and ecology of infectious hematopoietic necrosis virus (IHNV) in Pacific salmonids Host specificity and ecology of infectious hematopoietic necrosis virus (IHNV) in Pacific salmonids
Some circumstances IHNV infection can cause acute disease with mortality ranging from 5-90% in host populations. Genetic typing of IHNV field isolates has shown that three major genetic groups of the virus occur in North America. These groups are designated the U, M, and L virus genogroups because they occur in the upper, middle, and lower portions of the geographic range of IHNV in...
Authors
Gael Kurath, A. K. Garver, M. K. Purcell, Ma. Penaranda, Svetlana Rudakova
Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting Quantitative polymerase chain reaction (PCR) for detection of aquatic animal pathogens in a diagnostic laboratory setting
Real-time, or quantitative, polymerase chain reaction (qPCR) is quickly supplanting other molecular methods for detecting the nucleic acids of human and other animal pathogens owing to the speed and robustness of the technology. As the aquatic animal health community moves toward implementing national diagnostic testing schemes, it will need to evaluate how qPCR technology should be...
Authors
Maureen K. Purcell, Rodman G. Getchell, Carol A. McClure, S.E. Weber, Kyle A. Garver
Comparative evaluation of molecular diagnostic tests for Nucleospora salmonis and prevalence in migrating juvenile salmonids from the Snake River, USA Comparative evaluation of molecular diagnostic tests for Nucleospora salmonis and prevalence in migrating juvenile salmonids from the Snake River, USA
Nucleospora salmonis is an intranuclear microsporidian that primarily infects lymphoblast cells and contributes to chronic lymphoblastosis and a leukemia-like condition in a range of salmonid species. The primary goal of this study was to evaluate the prevalence of N. salmonis in out-migrating juvenile hatchery and wild Chinook salmon Oncorhynchus tshawytscha and steelhead O. mykiss from...
Authors
Samantha Badil, Diane G. Elliott, Tomofumi Kurobe, Ronald P. Hedrick, Kathy Clemens, Marilyn Blair, Maureen K. Purcell
Sequence analysis of the internal transcribed spacer (ITS) region reveals a novel clade of Ichthyophonus sp. from rainbow trout Sequence analysis of the internal transcribed spacer (ITS) region reveals a novel clade of Ichthyophonus sp. from rainbow trout
The mesomycetozoean parasite Ichthyophonus hoferi is most commonly associated with marine fish hosts but also occurs in some components of the freshwater rainbow trout Oncorhynchus mykiss aquaculture industry in Idaho, USA. It is not certain how the parasite was introduced into rainbow trout culture, but it might have been associated with the historical practice of feeding raw, ground...
Authors
C. Rasmussen, M. K. Purcell, J.L. Gregg, S. E. LaPatra, J. R. Winton, P.K. Hershberger
Non-USGS Publications**
Purcell, M., G. Mackey, E. LaHood, H. Huber, and L. Park. 2004. Molecular methods for the genetic identification of salmonid prey from Pacific harbor seal (Phoca vitulina richardsi ) scat. Fish. Bull. 1002: 213-220.
Lage, C., M. Purcell, M. Forgarty, and I. Kornfield. 2001. Microsatellite evaluation of haddock (Melanogrammus aeglefinus) stocks in the northwest Atlantic Ocean. Can. J. Fish. Aquat. Sci. 58: 982-990. DOI: 10.1139/cjfas-58-5-982.
Purcell, M.K., J-L. Mu, D.C. Higgins, R. Elango, H. Whitmore, S. Harris, and B. Paigen. 2001. Fine mapping of Ath6, a quantitative trait locus for atherosclerosis in mice. Mammalian Genome 12(7): 495-500. DOI: 10.1007/s00335001-0006-9.
Purcell, M.K., I.L. Kornfield, M.J. Fogarty and A. Parker. (1996) Interdecadal heterogeneity of mitochondrial DNA in Atlantic haddock (Melanogrammus aeglefinus) from Georges Bank. Molecular Marine Biology and Biotechnology. 5:185-192.
**Disclaimer: The views expressed in Non-USGS publications are those of the author and do not represent the views of the USGS, Department of the Interior, or the U.S. Government.
*Disclaimer: Listing outside positions with professional scientific organizations on this Staff Profile are for informational purposes only and do not constitute an endorsement of those professional scientific organizations or their activities by the USGS, Department of the Interior, or U.S. Government