Bob Rameyer is a Biological Laboratory Technician at the National Wildlife Health Center Honolulu Field Station.
Bob Rameyer has a BS degree in biology from Iowa State University. He worked as a technician for the US Department of Agriculture in Iowa and the National Wildlife Health Center in Wisconsin before coming to Hawaii in 1994. Bob heads up day to day operations of the Honolulu Field Station (HFS) and in general keeps the wheels on the cart.
Science and Products
Mass mortality of collector urchins (Tripneustes gratilla) in Hawai`i
As grazers, sea urchins are keystone species in tropical marine ecosystems, and their loss can have important ecological ramifications. Die-offs of urchins are frequently described but their causesare often unclear, in part because systematic examinations of animal tissues at gross and microscopic level are not done. In some areas, urchins are being employed to control invasive marine algae. Here
Data on invasive corallimorphs Palmyra
Invasive marine species are well documented but options to manage them are limited. At Palmyra Atoll National Wildlife Refuge (Central North Pacific), native invasive corallimorpharians, Rhodactis howesii, have smothered live native corals since 2007. Laboratory and field trials were conducted evaluating two control methods to remove R. howesii overgrowing the benthos at Palmyra Atoll (Palmyra): 1
Data set on reticuloendotheliosis in Hawaiian birds
Only one virus, Avipox, has been documented in wild birds in Hawaii. Here, using immunohistochemistry and PCR, we found that two native threatened Hawaiian geese, one with multicentric histiocytoma and another with toxoplasmosis and one Laysan albatross with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolated from one of the geese by cell culture. PCR surveys of
Data on blood cells of the collector urchin, Tripneustes gratilla
Echinoderms such as urchins are important in marine ecosystems, particularly as grazers, and unhealthy urchins can have important ecological implications. For instance, unexplained mortalities of Diadema sp. in the Caribbean were followed by algal overgrowth and subsequent collapse of coral reef ecosystems. Unfortunately few tools exist to evaluate echinoderm health making management of mortalitie
Pufferfish mortality data
In 2010, a mass mortality of pufferfish in Hawaii was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that 1) a marine toxin was associated with a kill of a fish species that is, itself, toxic; 2) we
Filter Total Items: 18
Mass mortality of collector urchins Tripneustes gratilla in Hawai`i
As grazers, sea urchins are keystone species in tropical marine ecosystems, and their loss can have important ecological ramifications. Die-offs of urchins are frequently described, but their causes are often unclear, in part because systematic examinations of animal tissues at gross and microscopic level are not done. In some areas, urchins are being employed to control invasive marine algae. Her
Authors
Thierry M. Work, Julie Dagenais, Robert Rameyer, Renee Breeden, Tina Weatherby
Invasive corallimorpharians at Palmyra Atoll National Wildlife Refuge are no match for lye and heat
Invasive marine species are well documented but options to manage them are limited. At Palmyra Atoll National Wildlife Refuge (Central North Pacific), native invasive corallimorpharians, Rhodactis howesii, have smothered live native corals since 2007. Laboratory and field trials were conducted evaluating two control methods to remove R. howesii overgrowing the benthos at Palmyra Atoll (Palmyra): 1
Authors
Thierry M. Work, Renee Breeden, Robert Rameyer, Vernon Born, Tim Clark, Jeremy Rainal, Chris Gillies, Julia Rose, Alex Wegmann, Stefan Kropidlowski
Morbidity and mortality of Hawaiin geese (Branta sandvicensis) and Laysan albatross (Phoebastria immutabilis) associated with reticuloendotheliosis virus
Only one virus, Avipox, has been documented previously in wild birds in Hawaii. Using immunohistochemistry and PCR, we found that two native threatened Hawaiian Geese (Branta sandvicensis), one with multicentric histiocytoma and the other with toxoplasmosis, and one Laysan Albatross (Phoebastria immutabilis) with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolate
Authors
Thierry M. Work, Renee Breeden, Julie Dagenais, Robert Rameyer, Holly Sellers, Hon S. Ip, James W. Casey
Cytology reveals diverse cell morphotypes and cellin-cell interactions in normal collector sea urchins Tripneustes gratilla
Echinoderms such as sea urchins are important in marine ecosystems, particularly as grazers, and unhealthy sea urchins can have important ecological implications. For instance, unexplained mortalities of Diadema antillarum in the Caribbean were followed by algal overgrowth and subsequent collapse of coral reef ecosystems. Unfortunately, few tools exist to evaluate echinoderm health, making managem
Authors
Thierry M. Work, Elena Millard, Daniela B. Mariani, Tina M. Weatherby, Robert Rameyer, Julie Dagenais, Renee Breeden, Allison Beale
A novel host-adapted strain of Salmonella Typhimurium causes disease in olive ridley turtles (Lepidochelys olivacea) in the Pacific.
Salmonella spp. are frequently shed by wildlife including turtles, but S. enterica subsp. enterica serovar Typhimurium or lesions associated with Salmonella are rare in turtles. Between 1996 and 2016, we necropsied 127 apparently healthy pelagic olive ridley turtles (Lepidochelys olivacea) that died from drowning bycatch in fisheries and 44 live or freshly dead stranded turtles from the west coast
Authors
Thierry M. Work, Julie Dagenais, Brian A. Stacy, Jason T. Ladner, Jeffrey M. Lorch, George H. Balazs, Elias Barquero-Calvo, Brenda M. Berlowski-Zier, Renee Breeden, Natalia Corrales-Gómez, Rocio Gonzalez-Barrientos, Heather Harris, Gabriela Hernández-Mora, Angel Herrera-Ulloa, Shoreh Hesami, T. Todd Jones, Juan Alberto Morales, Terry M. Norton, Robert Rameyer, Daniel Taylor, Thomas B. Waltzek
Pufferfish mortality associated with novel polar marine toxins in Hawaii
Fish die-offs are important signals in tropical marine ecosystems. In 2010, a mass mortality of pufferfish in Hawaii (USA) was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that (1) a marine toxin was
Authors
Thierry M. Work, Perer D. R. Moeller, Kevin R. Beauchesne, Julie Dagenais, Renee Breeden, Robert Rameyer, Willliam A. Walsh, Melanie Abecassis, Donald R. Kobayashi, Carla M. Conway, James Winton
Mortality patterns in endangered Hawaiian geese (Nene; Branta sandvicensis)
Understanding causes of death can aid management and recovery of endangered bird populations. Toward those ends, we systematically examined 300 carcasses of endangered Hawaiian Geese (Nene; Branta sandvicensis) from Hawaii, Maui, Molokai, and Kauai between 1992 and 2013. The most common cause of death was emaciation, followed by trauma (vehicular strikes and predation), and infectious/inflammatory
Authors
Thierry M. Work, Julie Dagenais, Robert Rameyer, Renee Breeden
Molecular phylogeny of Babesia poelea from brown boobies (Sula leucogaster) from Johnston Atoll, Central Pacific
The phylogenetic relationship of avian Babesia with other piroplasms remains unclear, mainly because of a lack of objective criteria such as molecular phylogenetics. In this study, our objective was to sequence the entire 18S, ITS-1, 5.8S, and ITS-2 regions of the rRNA gene and partial ß-tubulin gene of B. poelea, first described from brown boobies (Sula leucogaster) from the central Pacific, and
Authors
Michael J. Yabsley, Thierry M. Work, Robert A. Rameyer
Characterizing lesions in corals from American Samoa
The study of coral disease has suffered from an absence of systematic approaches that are commonly used to determine causes of diseases in animals. There is a critical need to develop a standardized and portable nomenclature for coral lesions in the field and to incorporate more commonly available biomedical tools in coral disease surveys to determine the potential causes of lesions in corals. We
Authors
Thierry M. Work, Robert A. Rameyer
Evaluating coral reef health in American Samoa
The study of coral disease has suffered from an absence of systematic approaches that are commonly used to determine causes of diseases in animals. There is a critical need to develop a standardized and portable nomenclature for coral lesions in the field and to incorporate more commonly available biomedical tools in coral disease surveys to determine the potential causes of lesions in corals. We
Authors
Thierry M. Work, Robert A. Rameyer
Retrospective pathology survey of green turtles Chelonia mydas with fibropapillomatosis in the Hawaiian Islands, 1993-2003
We necropsied 255 stranded green turtles Chelonia mydas with fibropapillomatosis (FP) from the Hawaiian Islands, North Pacific, from August 1993 through May 2003. Of these, 214 (84%) were euthanized due to advanced FP and the remainder were found dead in fresh condition. Turtles were assigned a standardized tumor severity score ranging from 1 (lightly tumored) to 3 (heavily tumored). Tumors were c
Authors
Thierry M. Work, George H. Balazs, Robert Rameyer, R.A. Morris
Protozoal and epitheliocystis-like infections in the introduced bluestripe snapper (Lutjanus kasmira) in Hawaii
The bluestripe snapper, or taape, was introduced into Hawaii in the 1950s and has since become very abundant throughout the archipelago. As part of a health survey of reef fish in Hawaii, we necropsied 120 taape collected from various coastal areas south of Oahu and examined fish histology for extraintestinal organisms. Forty-seven percent of taape were infected with an apicomplexan protozoan comp
Authors
Thierry M. Work, Robert Rameyer, Geraldine Takata, Michael L. Kent
Science and Products
- Data
Mass mortality of collector urchins (Tripneustes gratilla) in Hawai`i
As grazers, sea urchins are keystone species in tropical marine ecosystems, and their loss can have important ecological ramifications. Die-offs of urchins are frequently described but their causesare often unclear, in part because systematic examinations of animal tissues at gross and microscopic level are not done. In some areas, urchins are being employed to control invasive marine algae. HereData on invasive corallimorphs Palmyra
Invasive marine species are well documented but options to manage them are limited. At Palmyra Atoll National Wildlife Refuge (Central North Pacific), native invasive corallimorpharians, Rhodactis howesii, have smothered live native corals since 2007. Laboratory and field trials were conducted evaluating two control methods to remove R. howesii overgrowing the benthos at Palmyra Atoll (Palmyra): 1Data set on reticuloendotheliosis in Hawaiian birds
Only one virus, Avipox, has been documented in wild birds in Hawaii. Here, using immunohistochemistry and PCR, we found that two native threatened Hawaiian geese, one with multicentric histiocytoma and another with toxoplasmosis and one Laysan albatross with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolated from one of the geese by cell culture. PCR surveys ofData on blood cells of the collector urchin, Tripneustes gratilla
Echinoderms such as urchins are important in marine ecosystems, particularly as grazers, and unhealthy urchins can have important ecological implications. For instance, unexplained mortalities of Diadema sp. in the Caribbean were followed by algal overgrowth and subsequent collapse of coral reef ecosystems. Unfortunately few tools exist to evaluate echinoderm health making management of mortalitiePufferfish mortality data
In 2010, a mass mortality of pufferfish in Hawaii was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that 1) a marine toxin was associated with a kill of a fish species that is, itself, toxic; 2) we - Publications
Filter Total Items: 18
Mass mortality of collector urchins Tripneustes gratilla in Hawai`i
As grazers, sea urchins are keystone species in tropical marine ecosystems, and their loss can have important ecological ramifications. Die-offs of urchins are frequently described, but their causes are often unclear, in part because systematic examinations of animal tissues at gross and microscopic level are not done. In some areas, urchins are being employed to control invasive marine algae. HerAuthorsThierry M. Work, Julie Dagenais, Robert Rameyer, Renee Breeden, Tina WeatherbyInvasive corallimorpharians at Palmyra Atoll National Wildlife Refuge are no match for lye and heat
Invasive marine species are well documented but options to manage them are limited. At Palmyra Atoll National Wildlife Refuge (Central North Pacific), native invasive corallimorpharians, Rhodactis howesii, have smothered live native corals since 2007. Laboratory and field trials were conducted evaluating two control methods to remove R. howesii overgrowing the benthos at Palmyra Atoll (Palmyra): 1AuthorsThierry M. Work, Renee Breeden, Robert Rameyer, Vernon Born, Tim Clark, Jeremy Rainal, Chris Gillies, Julia Rose, Alex Wegmann, Stefan KropidlowskiMorbidity and mortality of Hawaiin geese (Branta sandvicensis) and Laysan albatross (Phoebastria immutabilis) associated with reticuloendotheliosis virus
Only one virus, Avipox, has been documented previously in wild birds in Hawaii. Using immunohistochemistry and PCR, we found that two native threatened Hawaiian Geese (Branta sandvicensis), one with multicentric histiocytoma and the other with toxoplasmosis, and one Laysan Albatross (Phoebastria immutabilis) with avian pox were infected with reticuloendotheliosis virus (REV). The virus was isolateAuthorsThierry M. Work, Renee Breeden, Julie Dagenais, Robert Rameyer, Holly Sellers, Hon S. Ip, James W. CaseyCytology reveals diverse cell morphotypes and cellin-cell interactions in normal collector sea urchins Tripneustes gratilla
Echinoderms such as sea urchins are important in marine ecosystems, particularly as grazers, and unhealthy sea urchins can have important ecological implications. For instance, unexplained mortalities of Diadema antillarum in the Caribbean were followed by algal overgrowth and subsequent collapse of coral reef ecosystems. Unfortunately, few tools exist to evaluate echinoderm health, making managemAuthorsThierry M. Work, Elena Millard, Daniela B. Mariani, Tina M. Weatherby, Robert Rameyer, Julie Dagenais, Renee Breeden, Allison BealeA novel host-adapted strain of Salmonella Typhimurium causes disease in olive ridley turtles (Lepidochelys olivacea) in the Pacific.
Salmonella spp. are frequently shed by wildlife including turtles, but S. enterica subsp. enterica serovar Typhimurium or lesions associated with Salmonella are rare in turtles. Between 1996 and 2016, we necropsied 127 apparently healthy pelagic olive ridley turtles (Lepidochelys olivacea) that died from drowning bycatch in fisheries and 44 live or freshly dead stranded turtles from the west coastAuthorsThierry M. Work, Julie Dagenais, Brian A. Stacy, Jason T. Ladner, Jeffrey M. Lorch, George H. Balazs, Elias Barquero-Calvo, Brenda M. Berlowski-Zier, Renee Breeden, Natalia Corrales-Gómez, Rocio Gonzalez-Barrientos, Heather Harris, Gabriela Hernández-Mora, Angel Herrera-Ulloa, Shoreh Hesami, T. Todd Jones, Juan Alberto Morales, Terry M. Norton, Robert Rameyer, Daniel Taylor, Thomas B. WaltzekPufferfish mortality associated with novel polar marine toxins in Hawaii
Fish die-offs are important signals in tropical marine ecosystems. In 2010, a mass mortality of pufferfish in Hawaii (USA) was dominated by Arothron hispidus showing aberrant neurological behaviors. Using pathology, toxinology, and field surveys, we implicated a series of novel, polar, marine toxins as a likely cause of this mass mortality. Our findings are striking in that (1) a marine toxin wasAuthorsThierry M. Work, Perer D. R. Moeller, Kevin R. Beauchesne, Julie Dagenais, Renee Breeden, Robert Rameyer, Willliam A. Walsh, Melanie Abecassis, Donald R. Kobayashi, Carla M. Conway, James WintonMortality patterns in endangered Hawaiian geese (Nene; Branta sandvicensis)
Understanding causes of death can aid management and recovery of endangered bird populations. Toward those ends, we systematically examined 300 carcasses of endangered Hawaiian Geese (Nene; Branta sandvicensis) from Hawaii, Maui, Molokai, and Kauai between 1992 and 2013. The most common cause of death was emaciation, followed by trauma (vehicular strikes and predation), and infectious/inflammatoryAuthorsThierry M. Work, Julie Dagenais, Robert Rameyer, Renee BreedenMolecular phylogeny of Babesia poelea from brown boobies (Sula leucogaster) from Johnston Atoll, Central Pacific
The phylogenetic relationship of avian Babesia with other piroplasms remains unclear, mainly because of a lack of objective criteria such as molecular phylogenetics. In this study, our objective was to sequence the entire 18S, ITS-1, 5.8S, and ITS-2 regions of the rRNA gene and partial ß-tubulin gene of B. poelea, first described from brown boobies (Sula leucogaster) from the central Pacific, andAuthorsMichael J. Yabsley, Thierry M. Work, Robert A. RameyerCharacterizing lesions in corals from American Samoa
The study of coral disease has suffered from an absence of systematic approaches that are commonly used to determine causes of diseases in animals. There is a critical need to develop a standardized and portable nomenclature for coral lesions in the field and to incorporate more commonly available biomedical tools in coral disease surveys to determine the potential causes of lesions in corals. WeAuthorsThierry M. Work, Robert A. RameyerEvaluating coral reef health in American Samoa
The study of coral disease has suffered from an absence of systematic approaches that are commonly used to determine causes of diseases in animals. There is a critical need to develop a standardized and portable nomenclature for coral lesions in the field and to incorporate more commonly available biomedical tools in coral disease surveys to determine the potential causes of lesions in corals. WeAuthorsThierry M. Work, Robert A. RameyerRetrospective pathology survey of green turtles Chelonia mydas with fibropapillomatosis in the Hawaiian Islands, 1993-2003
We necropsied 255 stranded green turtles Chelonia mydas with fibropapillomatosis (FP) from the Hawaiian Islands, North Pacific, from August 1993 through May 2003. Of these, 214 (84%) were euthanized due to advanced FP and the remainder were found dead in fresh condition. Turtles were assigned a standardized tumor severity score ranging from 1 (lightly tumored) to 3 (heavily tumored). Tumors were cAuthorsThierry M. Work, George H. Balazs, Robert Rameyer, R.A. MorrisProtozoal and epitheliocystis-like infections in the introduced bluestripe snapper (Lutjanus kasmira) in Hawaii
The bluestripe snapper, or taape, was introduced into Hawaii in the 1950s and has since become very abundant throughout the archipelago. As part of a health survey of reef fish in Hawaii, we necropsied 120 taape collected from various coastal areas south of Oahu and examined fish histology for extraintestinal organisms. Forty-seven percent of taape were infected with an apicomplexan protozoan compAuthorsThierry M. Work, Robert Rameyer, Geraldine Takata, Michael L. Kent