Sean Nashold (Former Employee)
Science and Products
Filter Total Items: 23
Evaluation of Nobuto filter paper strips for the detection of avian influenza virus antibody in waterfowl Evaluation of Nobuto filter paper strips for the detection of avian influenza virus antibody in waterfowl
The utility of using Nobuto paper strips for the detection of avian influenza antibodies was examined in mallards (Anas platyrhynchos) experimentally infected with low pathogenic avian influenza viruses. Blood was collected 2 wk after infection and was preserved either as serum or whole blood absorbed onto Nobuto strips. Analysis of samples using a commercially available blocking enzyme...
Authors
Robert J. Dusek, Jeffrey S. Hall, Sean W. Nashold, Joshua L. Teslaa, Hon S. Ip
Experimental infection of a North American raptor, American kestrel (Falco sparverius), with highly pathogenic avian influenza virus (H5N1) Experimental infection of a North American raptor, American kestrel (Falco sparverius), with highly pathogenic avian influenza virus (H5N1)
Several species of wild raptors have been found in Eurasia infected with highly pathogenic avian influenza virus (HPAIV) subtype H5N1. Should HPAIV (H5N1) reach North America in migratory birds, species of raptors are at risk not only from environmental exposure, but also from consuming infected birds and carcasses. In this study we used American kestrels as a representative species of a...
Authors
Jeffrey S. Hall, Hon S. Ip, J. C. Franson, C. Meteyer, Sean W. Nashold, Joshua L. Teslaa, J. French, P. Redig, C. Brand
Diagnosis of duck plague in waterfowl by polymerase chain reaction Diagnosis of duck plague in waterfowl by polymerase chain reaction
A recently developed polymerase chain reaction (PCR) assay was used for diagnosis of duck plague in waterfowl tissues from past and current cases of waterfowl mortality and to identify duck plague virus in combined cloacal/oral-pharyngeal swab samples from healthy mallards (Anas platyrhynchos) after a disease outbreak. The PCR was able to detect viral DNA from all the individual or...
Authors
W. R. Hansen, S.W. Nashold, D. E. Docherty, S.E. Brown, D.L. Knudson
Identification of duck plague virus by polymerase chain reaction Identification of duck plague virus by polymerase chain reaction
A polymerase chain reaction (PCR) assay was developed for detecting duck plague virus. A 765-bp EcoRI fragment cloned from the genome of the duck plague vaccine (DP-VAC) virus was sequenced for PCR primer development. The fragment sequence was found by GenBank alignment searches to be similar to the 3′ ends of an undefined open reading frame and the gene for DNA polymerase protein in...
Authors
W. R. Hansen, Sean E. Brown, S.W. Nashold, D.L. Knudson
Preliminary evaluation of a simple in vitro test for the diagnosis of type C botulism in wild birds Preliminary evaluation of a simple in vitro test for the diagnosis of type C botulism in wild birds
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of type C botulinum toxin (Clostridium botulinum) in wild birds. This simple, antigen-capture ELISA utilizes polystyrene immunosticks as the solid substrate, chicken antitoxin (IgY) as the coating antibody, rabbit antitoxin as the primary antibody, and peroxidase-labeled goat-anti-rabbit as the secondary...
Authors
Tonie E. Rocke, Susan Smith, Sean Nashold
Science and Products
Filter Total Items: 23
Evaluation of Nobuto filter paper strips for the detection of avian influenza virus antibody in waterfowl Evaluation of Nobuto filter paper strips for the detection of avian influenza virus antibody in waterfowl
The utility of using Nobuto paper strips for the detection of avian influenza antibodies was examined in mallards (Anas platyrhynchos) experimentally infected with low pathogenic avian influenza viruses. Blood was collected 2 wk after infection and was preserved either as serum or whole blood absorbed onto Nobuto strips. Analysis of samples using a commercially available blocking enzyme...
Authors
Robert J. Dusek, Jeffrey S. Hall, Sean W. Nashold, Joshua L. Teslaa, Hon S. Ip
Experimental infection of a North American raptor, American kestrel (Falco sparverius), with highly pathogenic avian influenza virus (H5N1) Experimental infection of a North American raptor, American kestrel (Falco sparverius), with highly pathogenic avian influenza virus (H5N1)
Several species of wild raptors have been found in Eurasia infected with highly pathogenic avian influenza virus (HPAIV) subtype H5N1. Should HPAIV (H5N1) reach North America in migratory birds, species of raptors are at risk not only from environmental exposure, but also from consuming infected birds and carcasses. In this study we used American kestrels as a representative species of a...
Authors
Jeffrey S. Hall, Hon S. Ip, J. C. Franson, C. Meteyer, Sean W. Nashold, Joshua L. Teslaa, J. French, P. Redig, C. Brand
Diagnosis of duck plague in waterfowl by polymerase chain reaction Diagnosis of duck plague in waterfowl by polymerase chain reaction
A recently developed polymerase chain reaction (PCR) assay was used for diagnosis of duck plague in waterfowl tissues from past and current cases of waterfowl mortality and to identify duck plague virus in combined cloacal/oral-pharyngeal swab samples from healthy mallards (Anas platyrhynchos) after a disease outbreak. The PCR was able to detect viral DNA from all the individual or...
Authors
W. R. Hansen, S.W. Nashold, D. E. Docherty, S.E. Brown, D.L. Knudson
Identification of duck plague virus by polymerase chain reaction Identification of duck plague virus by polymerase chain reaction
A polymerase chain reaction (PCR) assay was developed for detecting duck plague virus. A 765-bp EcoRI fragment cloned from the genome of the duck plague vaccine (DP-VAC) virus was sequenced for PCR primer development. The fragment sequence was found by GenBank alignment searches to be similar to the 3′ ends of an undefined open reading frame and the gene for DNA polymerase protein in...
Authors
W. R. Hansen, Sean E. Brown, S.W. Nashold, D.L. Knudson
Preliminary evaluation of a simple in vitro test for the diagnosis of type C botulism in wild birds Preliminary evaluation of a simple in vitro test for the diagnosis of type C botulism in wild birds
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of type C botulinum toxin (Clostridium botulinum) in wild birds. This simple, antigen-capture ELISA utilizes polystyrene immunosticks as the solid substrate, chicken antitoxin (IgY) as the coating antibody, rabbit antitoxin as the primary antibody, and peroxidase-labeled goat-anti-rabbit as the secondary...
Authors
Tonie E. Rocke, Susan Smith, Sean Nashold