Tonie Rocke
Tonie Rocke is a Research Epidemiologist at the National Wildlife Health Center.
Science and Products
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Consumption of baits containing raccoon pox-based plague vaccines protects black-tailed prairie dogs (Cynomys ludovicianus) Consumption of baits containing raccoon pox-based plague vaccines protects black-tailed prairie dogs (Cynomys ludovicianus)
Baits containing recombinant raccoon poxvirus (RCN) expressing plague antigens (fraction 1 [F1] and a truncated form of the V protein-V307) were offered for voluntary consumption several times over the course of several months to a group of 16 black-tailed prairie dogs (Cynomys ludovicianus). For comparison, another group of prairie dogs (n = 12) was injected subcutaneously (SC) (prime...
Authors
Tonie E. Rocke, Nicola Pussini, Susan Smith, Judy L. Williamson, Bradford Powell, Jorge E. Osorio
Enzootic plague reduces black-footed ferret (Mustela nigripes) survival in Montana Enzootic plague reduces black-footed ferret (Mustela nigripes) survival in Montana
Black-footed ferrets (Mustela nigripes) require extensive prairie dog colonies (Cynomys spp.) to provide habitat and prey. Epizootic plague kills both prairie dogs and ferrets and is a major factor limiting recovery of the highly endangered ferret. In addition to epizootics, we hypothesized that enzootic plague, that is, presence of disease-causing Yersinia pestis without any noticeable...
Authors
Marc R. Matchett, Dean E. Biggins, Valerie Carlson, Bradford Powell, Tonie E. Rocke
Further development of raccoon poxvirus-vectored vaccines against plague (Yersinia pestis) Further development of raccoon poxvirus-vectored vaccines against plague (Yersinia pestis)
In previous studies, we demonstrated protection against plague in mice and prairie dogs using a raccoon pox (RCN) virus-vectored vaccine that expressed the F1 capsular antigen of Yersinia pestis. In order to improve vaccine efficacy, we have now constructed additional RCN-plague vaccines containing two different forms of the lcrV (V) gene, including full-length (Vfull) and a truncated...
Authors
Tonie E. Rocke, Keith P. Iams, S. Dawe, Susan Smith, Judy L. Williamson, Dennis M. Heisey, Jorge E. Osorio
Comparison of monkeypox viruses pathogenesis in mice by in vivo imaging Comparison of monkeypox viruses pathogenesis in mice by in vivo imaging
Monkeypox viruses (MPXV) cause human monkeypox, a zoonotic smallpox-like disease endemic to Africa, and are of worldwide public health and biodefense concern. Using viruses from the Congo (MPXV-2003-Congo-358) and West African (MPXV-2003-USA-044) clades, we constructed recombinant viruses that express the luciferase gene (MPXV-Congo/Luc+and MPXV-USA-Luc+) and compared their viral...
Authors
Jorge E. Osorio, Keith P. Iams, Carol U. Meteyer, Tonie E. Rocke
Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows
Three adult lactating Holstein cows were injected in the subcutaneous abdominal vein with 175 ng/kg of body weight of Clostridium botulinum type C toxin (451 cow median toxic doses) to determine if this botulinum toxin crosses the blood–milk barrier. Whole blood (in sodium heparin) and clotted blood serum samples were taken at 0 min, 10 min, and 3, 6, 9, and 12 h postinoculation. Milk...
Authors
R.B. Moeller, B. Puschner, R.L. Walker, Tonie E. Rocke, S.R. Smith, J.S. Cullor, A.A. Ardans
Protecting Black-Footed Ferrets and Prairie Dogs against sylvatic plague Protecting Black-Footed Ferrets and Prairie Dogs against sylvatic plague
Scientists at the USGS National Wildlife Health Center (NWHC), in collaboration with colleagues at other federal agencies and the University of Wisconsin, are developing and testing vaccines that can be used to protect black-footed ferrets and prairie dogs against plague. The black-footed ferret is commonly regarded as the most endangered mammal in North America, and sylvatic plague is a...
Authors
Tonie E. Rocke
Science and Products
Filter Total Items: 16
No Result Found
Filter Total Items: 134
Consumption of baits containing raccoon pox-based plague vaccines protects black-tailed prairie dogs (Cynomys ludovicianus) Consumption of baits containing raccoon pox-based plague vaccines protects black-tailed prairie dogs (Cynomys ludovicianus)
Baits containing recombinant raccoon poxvirus (RCN) expressing plague antigens (fraction 1 [F1] and a truncated form of the V protein-V307) were offered for voluntary consumption several times over the course of several months to a group of 16 black-tailed prairie dogs (Cynomys ludovicianus). For comparison, another group of prairie dogs (n = 12) was injected subcutaneously (SC) (prime...
Authors
Tonie E. Rocke, Nicola Pussini, Susan Smith, Judy L. Williamson, Bradford Powell, Jorge E. Osorio
Enzootic plague reduces black-footed ferret (Mustela nigripes) survival in Montana Enzootic plague reduces black-footed ferret (Mustela nigripes) survival in Montana
Black-footed ferrets (Mustela nigripes) require extensive prairie dog colonies (Cynomys spp.) to provide habitat and prey. Epizootic plague kills both prairie dogs and ferrets and is a major factor limiting recovery of the highly endangered ferret. In addition to epizootics, we hypothesized that enzootic plague, that is, presence of disease-causing Yersinia pestis without any noticeable...
Authors
Marc R. Matchett, Dean E. Biggins, Valerie Carlson, Bradford Powell, Tonie E. Rocke
Further development of raccoon poxvirus-vectored vaccines against plague (Yersinia pestis) Further development of raccoon poxvirus-vectored vaccines against plague (Yersinia pestis)
In previous studies, we demonstrated protection against plague in mice and prairie dogs using a raccoon pox (RCN) virus-vectored vaccine that expressed the F1 capsular antigen of Yersinia pestis. In order to improve vaccine efficacy, we have now constructed additional RCN-plague vaccines containing two different forms of the lcrV (V) gene, including full-length (Vfull) and a truncated...
Authors
Tonie E. Rocke, Keith P. Iams, S. Dawe, Susan Smith, Judy L. Williamson, Dennis M. Heisey, Jorge E. Osorio
Comparison of monkeypox viruses pathogenesis in mice by in vivo imaging Comparison of monkeypox viruses pathogenesis in mice by in vivo imaging
Monkeypox viruses (MPXV) cause human monkeypox, a zoonotic smallpox-like disease endemic to Africa, and are of worldwide public health and biodefense concern. Using viruses from the Congo (MPXV-2003-Congo-358) and West African (MPXV-2003-USA-044) clades, we constructed recombinant viruses that express the luciferase gene (MPXV-Congo/Luc+and MPXV-USA-Luc+) and compared their viral...
Authors
Jorge E. Osorio, Keith P. Iams, Carol U. Meteyer, Tonie E. Rocke
Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows Attempts to identify Clostridium botulinum toxin in milk from three experimentally intoxicated Holstein cows
Three adult lactating Holstein cows were injected in the subcutaneous abdominal vein with 175 ng/kg of body weight of Clostridium botulinum type C toxin (451 cow median toxic doses) to determine if this botulinum toxin crosses the blood–milk barrier. Whole blood (in sodium heparin) and clotted blood serum samples were taken at 0 min, 10 min, and 3, 6, 9, and 12 h postinoculation. Milk...
Authors
R.B. Moeller, B. Puschner, R.L. Walker, Tonie E. Rocke, S.R. Smith, J.S. Cullor, A.A. Ardans
Protecting Black-Footed Ferrets and Prairie Dogs against sylvatic plague Protecting Black-Footed Ferrets and Prairie Dogs against sylvatic plague
Scientists at the USGS National Wildlife Health Center (NWHC), in collaboration with colleagues at other federal agencies and the University of Wisconsin, are developing and testing vaccines that can be used to protect black-footed ferrets and prairie dogs against plague. The black-footed ferret is commonly regarded as the most endangered mammal in North America, and sylvatic plague is a...
Authors
Tonie E. Rocke