Increased salinization of freshwater systems is a growing concern, and can be attributed to a variety of factors including climate change, land-use change, agricultural practices, road de-icing, and brines released from fossil fuel extraction. Effects of increased salinization on aquatic organisms is little understood and may be vastly different among species and among different life stages.
Researchers at the USGS Northern Appalachian Research Laboratory are using a combined laboratory, physiology, and genomics approach to investigate these effects in several key freshwater species including brook trout (Salvelinus fontinalis), American eel (Anguilla rostrata), eastern elliptio mussel (Elliptio complanata), and the federally listed northern riffleshell mussel (Epioblasma torulosa rangiana). Due to freshwater mussels’ complex life cycle, studies are being conducted with adults as well as more sensitive juvenile and larval stages. Studies allow insight into the mechanisms involved in a species’ response to increased salinization, determine environmental conditions that elicit a biologically relevant response, and develop meaningful biomarkers for field application and use in management
Linking otolith microchemistry and surface water contamination from natural gas mining
Exposure to synthetic hydraulic fracturing waste influences the mucosal bacterial community structure of the brook trout (Salvelinus fontinalis) epidermis
Draft genome sequence of a picorna-like virus associated with gill tissue in clinically normal brook trout, Salvelinus fontinalis
RNA sequencing analysis of transcriptional change in the freshwater mussel Elliptio complanata after environmentally relevant sodium chloride exposure
Transcriptomic analysis of the mussel Elliptio complanata identifies candidate stress-response genes and an abundance of novel or noncoding transcripts
The effects of salinity exposure on multiple life stages of a common freshwater mussel, Elliptio complanata
- Overview
Increased salinization of freshwater systems is a growing concern, and can be attributed to a variety of factors including climate change, land-use change, agricultural practices, road de-icing, and brines released from fossil fuel extraction. Effects of increased salinization on aquatic organisms is little understood and may be vastly different among species and among different life stages.
Adult northern riffleshell mussels (Epioblasma torulosa rangiana) experimentally exposed to increased chloride levels. Researchers at the USGS Northern Appalachian Research Laboratory are using a combined laboratory, physiology, and genomics approach to investigate these effects in several key freshwater species including brook trout (Salvelinus fontinalis), American eel (Anguilla rostrata), eastern elliptio mussel (Elliptio complanata), and the federally listed northern riffleshell mussel (Epioblasma torulosa rangiana). Due to freshwater mussels’ complex life cycle, studies are being conducted with adults as well as more sensitive juvenile and larval stages. Studies allow insight into the mechanisms involved in a species’ response to increased salinization, determine environmental conditions that elicit a biologically relevant response, and develop meaningful biomarkers for field application and use in management
Digital pictures were taken each week of juvenile northern riffleshell mussel (Epioblasma torulosa rangiana) to monitor growth throughout experimental exposure to chloride. Individual’s length and width were measured using a computer software program and compared among chloride treatments to determine if chloride exposure impacted juvenile mussel growth. Brook trout were exposed to low concentrations of synthetic brine from the natural gas extraction process to evaluate the effects of on their survival, physiology, and body condition. - Publications
Linking otolith microchemistry and surface water contamination from natural gas mining
Unconventional natural gas drilling and the use of hydraulic fracturing technology have expanded rapidly in North America. This expansion has raised concerns of surface water contamination by way of spills and leaks, which may be sporadic, small, and therefore difficult to detect. Here we explore the use of otolith microchemistry as a tool for monitoring surface water contamination from generatedAuthorsDavid H. Keller, Paula M. Zelanko, Joel E. Gagnon, Richard J. Horwitz, Heather S. Galbraith, David J. VelinskyExposure to synthetic hydraulic fracturing waste influences the mucosal bacterial community structure of the brook trout (Salvelinus fontinalis) epidermis
Production of natural gas using unconventional technologies has risen as demand for alternative fuels has increased. Impacts on the environment from waste generated from these processes are largely unexplored. In particular, the outcomes of organismal exposure to hydraulic fracturing waste have not been rigorously evaluated. We evaluated the effects of exposure to surrogate hydraulic fracturing waAuthorsHeather S. Galbraith, Deborah D. Iwanowicz, Daniel E. Spooner, Luke R. Iwanowicz, David Keller, Paula M. Zelanko, Cynthia R. AdamsDraft genome sequence of a picorna-like virus associated with gill tissue in clinically normal brook trout, Salvelinus fontinalis
Here, we report a draft genome sequence of a picorna-like virus associated with brook trout, Salvelinus fontinalis, gill tissue. The draft genome comprises 8,681 nucleotides, excluding the poly(A) tract, and contains two open reading frames. It is most similar to picorna-like viruses that infect invertebrates.AuthorsLuke R. Iwanowicz, Deborah D. Iwanowicz, Cynthia R. Adams, Heather S. Galbraith, Aaron Aunins, Robert S. CornmanRNA sequencing analysis of transcriptional change in the freshwater mussel Elliptio complanata after environmentally relevant sodium chloride exposure
To identify potential biomarkers of salt stress in a freshwater sentinel species, we examined transcriptional responses of the common mussel Elliptio complanata to controlled sodium chloride (NaCl) exposures. Ribonucleic acid sequencing (RNA-Seq) of mantle tissue identified 481 transcripts differentially expressed in adult mussels exposed to 2 ppt NaCl (1.2 ppt chloride) for 7 d, of which 290 hadAuthorsLaura S. Robertson, Heather S. Galbraith, Deborah D. Iwanowicz, Carrie J. Blakeslee, Robert S. CornmanTranscriptomic analysis of the mussel Elliptio complanata identifies candidate stress-response genes and an abundance of novel or noncoding transcripts
Mussels are useful indicator species of environmental stress and degradation, and the global decline in freshwater mussel diversity and abundance is of conservation concern. Elliptio complanata is a common freshwater mussel of eastern North America that can serve both as an indicator and as an experimental model for understanding mussel physiology and genetics. To support genetic components of theAuthorsRobert S. Cornman, Laura S. Robertson, Heather S. Galbraith, Carrie J. BlakesleeThe effects of salinity exposure on multiple life stages of a common freshwater mussel, Elliptio complanata
There is growing concern over the effects of increased salinization on freshwater organisms, which are largely unknown for unionid mussels. Adult and larval Elliptio complanata were exposed to low-level salt concentrations to determine the effects on mussel survival, physiology, and reproduction. Adults were exposed to salt concentrations of 0 parts per thousand (ppt), 2 ppt, 4 ppt, and 6 ppt NaClAuthorsCarrie J. Blakeslee, Heather S. Galbraith, Laura S. Robertson, Barbara St. John White