Assessing Impacts of Emerging and Established Diseases to Aquatic Ecosystems
Native freshwater mussels play a critical role in aquatic environments and are considered “ecosystem engineers” and indicators of water quality by constantly filtering water. Populations of native freshwater mussels have declined in recent years, and this decline has been attributed to factors such as habitat degradation, pollution, and invasive species, among others. The importance of these organisms is recognized, and significant efforts are being made by USGS researchers, along with conservation agencies, to conserve and restore the populations of native freshwater mussels. Efforts are being focused on developing sample protocols and diagnostic tools to assess mussel health, developing technology to survey a broad array of aquatic pathogens, and investigating the causes behind the mass mussel die-offs being seen around the U.S.
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Investigation Into Mass Mussel Die-off Events
Principal Investigator – Diane Waller
The decline of native freshwater mussels has been attributed to factors such as habitat degradation, pollution, and invasive species. Although factors such as habitat degradation, pollution, and invasive species have been linked to this decline, these potential causes cannot fully explain the large-scale mussel die-offs that have occurred in the past 20 years. These die-offs have taken place in relatively “healthy” streams across the U.S. and occasionally only one or a few species out of many are affected. Several of these mass mussel mortality events have involved federal and state-listed species. Mass mortality in the Clinch River, Tennessee and Virginia, has been ongoing since 2016 and threatens the U.S. Fish and Wildlife Service’s efforts to restore imperiled species in the Upper Tennessee River Basin. In these and other cases, no cause of the die-off has been identified, despite patterns of spread that suggest a disease process in which an infectious agent or agents may be involved. However, these potential causes cannot fully explain the large-scale mussel die-offs that have occurred in the past 20 years. USGS and partners are investigating underlying causes for the mass mortalities.
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Development of Sampling Protocols and Diagnostic Tools for Assessment of Freshwater Mussel Health
Principal Investigator – Diane Waller
The role of disease in freshwater mussel declines has been largely ignored due to the lack of appropriate diagnostic tools. Furthermore, health assessment of mussels in conjunction with restoration and propagation has not been considered during stocking and augmentation activities. This project will develop standard techniques for nonlethal sampling of freshwater mussels to diagnose disease and assess health condition. The techniques and protocols developed from the project will provide a framework for standardized health monitoring of freshwater mollusk populations in any water body and propagation facility.
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Below are publications associated with this project.
Targeted metabolomics characterizes metabolite occurrence and variability in stable freshwater mussel populations
A bacteriological comparison of the hemolymph from healthy and moribund unionid mussel populations in the upper Midwestern U.S.A. prompts the development of diagnostic assays to detect Yokenella regensburgei
Further bacteriological analysis of annual Pheasantshell (Actinonaias pectorosa) mussel mortality events in the Clinch River (Virginia/Tennessee), USA, reveals a consistent association with Yokenella Regensburgei
Freshwater mussels show elevated viral richness and intensity during a mortality event
A novel gonadotropic microsporidian parasite (Microsporidium clinchi n. sp.) infecting a declining population of pheasantshell mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA
Mussel mass mortality and the microbiome: Evidence for shifts in the bacterial microbiome of a declining freshwater bivalve
Mass mortality in freshwater mussels (Actinonaias pectorosa) in the Clinch River, USA, linked to a novel densovirus
The status of mussel health assessment and a path forward
A novel picorna-like virus in a Wabash Pigtoe (Fusconaia flava) from the upper Mississippi River, USA
A comparison of bacteria cultured from unionid mussel hemolymph between stable populations in the upper Mississippi River and a mortality event in the Clinch River
Building a response network to investigate potential pathogens associated with unionid mortality events
Below are news stories associated with this project.
Native freshwater mussels play a critical role in aquatic environments and are considered “ecosystem engineers” and indicators of water quality by constantly filtering water. Populations of native freshwater mussels have declined in recent years, and this decline has been attributed to factors such as habitat degradation, pollution, and invasive species, among others. The importance of these organisms is recognized, and significant efforts are being made by USGS researchers, along with conservation agencies, to conserve and restore the populations of native freshwater mussels. Efforts are being focused on developing sample protocols and diagnostic tools to assess mussel health, developing technology to survey a broad array of aquatic pathogens, and investigating the causes behind the mass mussel die-offs being seen around the U.S.
--------------------------------------------
Investigation Into Mass Mussel Die-off Events
Principal Investigator – Diane Waller
The decline of native freshwater mussels has been attributed to factors such as habitat degradation, pollution, and invasive species. Although factors such as habitat degradation, pollution, and invasive species have been linked to this decline, these potential causes cannot fully explain the large-scale mussel die-offs that have occurred in the past 20 years. These die-offs have taken place in relatively “healthy” streams across the U.S. and occasionally only one or a few species out of many are affected. Several of these mass mussel mortality events have involved federal and state-listed species. Mass mortality in the Clinch River, Tennessee and Virginia, has been ongoing since 2016 and threatens the U.S. Fish and Wildlife Service’s efforts to restore imperiled species in the Upper Tennessee River Basin. In these and other cases, no cause of the die-off has been identified, despite patterns of spread that suggest a disease process in which an infectious agent or agents may be involved. However, these potential causes cannot fully explain the large-scale mussel die-offs that have occurred in the past 20 years. USGS and partners are investigating underlying causes for the mass mortalities.
------------------------------------------
Development of Sampling Protocols and Diagnostic Tools for Assessment of Freshwater Mussel Health
Principal Investigator – Diane Waller
The role of disease in freshwater mussel declines has been largely ignored due to the lack of appropriate diagnostic tools. Furthermore, health assessment of mussels in conjunction with restoration and propagation has not been considered during stocking and augmentation activities. This project will develop standard techniques for nonlethal sampling of freshwater mussels to diagnose disease and assess health condition. The techniques and protocols developed from the project will provide a framework for standardized health monitoring of freshwater mollusk populations in any water body and propagation facility.
--------------------------------------
Below are publications associated with this project.
Targeted metabolomics characterizes metabolite occurrence and variability in stable freshwater mussel populations
A bacteriological comparison of the hemolymph from healthy and moribund unionid mussel populations in the upper Midwestern U.S.A. prompts the development of diagnostic assays to detect Yokenella regensburgei
Further bacteriological analysis of annual Pheasantshell (Actinonaias pectorosa) mussel mortality events in the Clinch River (Virginia/Tennessee), USA, reveals a consistent association with Yokenella Regensburgei
Freshwater mussels show elevated viral richness and intensity during a mortality event
A novel gonadotropic microsporidian parasite (Microsporidium clinchi n. sp.) infecting a declining population of pheasantshell mussels (Actinonaias pectorosa) (Unioinidae) from the Clinch River, USA
Mussel mass mortality and the microbiome: Evidence for shifts in the bacterial microbiome of a declining freshwater bivalve
Mass mortality in freshwater mussels (Actinonaias pectorosa) in the Clinch River, USA, linked to a novel densovirus
The status of mussel health assessment and a path forward
A novel picorna-like virus in a Wabash Pigtoe (Fusconaia flava) from the upper Mississippi River, USA
A comparison of bacteria cultured from unionid mussel hemolymph between stable populations in the upper Mississippi River and a mortality event in the Clinch River
Building a response network to investigate potential pathogens associated with unionid mortality events
Below are news stories associated with this project.