Publications

Initially established from proliferative skin lesions of the common carp, Cyprinus carpio L., the epithelioma papulosum cyprini (EPC) cell line (Fijan, Sulimanovic, Bearzotti, Muzinic, Zwillenberg, Chilmonczyk, Vautherot & de Kinkelin 1983) has become one of the most widely used tools for research on fish viruses and the diagnosis of fish viral diseases.

In fisheries science, habitat use is often inferred when fish are sampled or observed in a particular location. Physical habitat is typically measured where fish are found, and thus deemed important to habitat use. Although less common, a more informative approach is to measure or observe fish behavior within given habitats to more thoroughly assess their use of those locations. While...

A candidate vaccine against Aeromonas hydrophila in rainbow trout, Oncorhynchus mykiss, was developed using a bacterial lysate. To test the strength of protection, A. hydrophila challenge models were compared using injection into both the intraperitoneal (IP) cavity and the dorsal sinus (DS) with selected doses of live bacteria washed in saline or left untreated. Unlike the IP route...

Losses from infectious diseases are an important component of natural mortality among marine fish species, but factors controlling the ecology of these diseases and their potential responses to anthropogenic changes are poorly understood. We used viral hemorrhagic septicemia virus (VHSV) and a laboratory stock of Pacific herring Clupea pallasii to investigate the kinetics of viral...

For more than a decade, investigators from different research groups in the Pacific Northwest have been using electronic tags to estimate survival of salmonid smolts as they migrate seaward past hydroelectric dams and through impoundments on the Snake and Columbia Rivers. Over the years, they have refined both analytical and field methods associated with such studies. In this...

Chinook salmon Oncorhynchus tshawytscha are highly susceptible to Renibacterium salmoninarum, the causative agent of bacterial kidney disease (BKD). Previously we demonstrated that introduced Chinook salmon from Lake Michigan, Wisconsin (WI), USA, have higher survival following R. salmoninarum challenge relative to the progenitor stock from Green River, Washington, USA. In the present...

We evaluated behavior, passage, and survival of juvenile salmonids at McNary Dam in relation to the temporary spillway weirs (TSWs) using acoustic telemetry during 2009. The TSWs were located in spill bays 4 and 20 during spring and in spill bays 19 and 20 during summer. Our objectives were to assess the performance of the TSWs as a fish passage alternative. We also examined how tailrace

McNary Lock and Dam were completed in 1953, creating McNary Reservoir, or Lake Wallula. The shoreline of the reservoir is federally owned and as a result the U.S. Army Corps of Engineers (USACE) has certain land and fish habitat management responsibilities to balance with other multipurpose benefits. The Endangered Species Act (ESA) listing of Columbia and Snake River salmon stocks has...

Induction of the innate immune pathways is critical for early anti-viral defense but there is limited understanding of how teleost fish recognize viral molecules and activate these pathways. In mammals, Toll-like receptors (TLR) 7 and 8 bind single-stranded RNA of viral origin and are activated by synthetic anti-viral imidazoquinoline compounds. Herein, we identify and describe the...

During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales...

Infectious haematopoietic necrosis virus (IHNV) is one of the most important viral pathogens of salmonids. In rainbow trout, IHNV isolates in the M genogroup are highly pathogenic, while U genogroup isolates are significantly less pathogenic. We show here that, at a multiplicity of infection (MOI) of 1, a representative U type strain yielded 42‐fold less infectious virus than an M type...

This report summarizes work completed by U.S. Geological Survey’s Columbia River Research Laboratory (USGS-CRRL) in the Wind River subbasin during the period November 2008 through October 2009 under Bonneville Power Administration (BPA) contract 41038. Long term research in the Wind River has focused on assessments of steelhead/rainbow trout Oncorhynchus mykiss populations, interactions...