Data release for determination of antimycin–a in liquid formulation by high performance liquid chromatography–mass spectrometry

Pesticide formulations containing the active ingredient Antimycin-A (ANT-A) have been used by fisheries and aquaculture managers for several decades to remove nuisance fish species. Analytical methods for measuring ANT-A during pesticide treatments have been reported using High Performance Liquid Chromatography (HPLC) paired with multiple detection methods (e.g., electrochemical, ultraviolet, fluorescence, mass spectrometry). However, instruments and analytical chemistry methods can advance over time due to the need to develop timely, reliable, cost effective, and reproducible methods. Subsequently, ANT-A analytical chemistry methods and sample processing techniques have also improved over the past several decades. In the present study, we describe a Liquid Chromatography - Mass Spectroscopy (LC-MS/MS) method and its verification across three separate analysts. Each analyst group created a single calibration curve and verified ANT-A in a liquid formulation using the averaged total response of all major ANT-A homologs (A1, A3, A3, A4). The advantage of this technique creates a more resilient ANT-A quantification method amendable to batch-batch differences in major homologs. The method demonstrated how ANT-A can be effectively measured with high accuracy (98 to 99%), precision (2.7 to 16.2%), and specificity within a pesticide liquid formulation using a method applicable for federal registration requirements. The dataset includes raw files of Antimycin-A analytical measurements. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.