John Steiner preparing to bring wastewater samples to FedEx for shipping.
Nolan Steiner
Nolan Steiner
Science and Products
Data release for determination of antimycin–a in liquid formulation by high performance liquid chromatography–mass spectrometry
Pesticide formulations containing the active ingredient Antimycin-A (ANT-A) have been used by fisheries and aquaculture managers for several decades to remove nuisance fish species. Analytical methods for measuring ANT-A during pesticide treatments have been reported using High Performance Liquid Chromatography (HPLC) paired with multiple detection methods (e.g., electrochemical, ultraviolet, fluo
Data release for toxicity of Antimycin A incorporated management bait for grass carp
The goal of this study was to develop and examine whether a management bait that can be used for selective control of grass carp. Our objectives were to 1) quantify the water-based 24-h LC50 of Antimycin-A for grass carp and rainbow trout, 2) quantify the 96-h LD50 of orally administered Antimycin-A laden bait for grass carp and rainbow trout, 3) quantify the leaching rate of Antimycin-A from the
Metabolomic analysis of pheasantshell mussel (Ortmanniana pectorosa; Order Unionida) from a mass mortality event in the Clinch River, Virginia and Tennessee, USA
This dataset uses the ITIS recommended Ortmanniana pectorosa, Conrad, 1834 in place of Actinonaias pectorosa, which was used in the Richard et al. 2020 manuscript. Biologists monitoring freshwater mussel (Order Unionida) populations rely on behavioral, often subjective, symptoms to identify “sick” or stressed animals, such as gaping valves and slow response to probing and lack clinical indicators
Characterization of occurrence and abundance of targeted metabolites in unionid mussels from three streams
The data consists of targeted metabolite analysis (using quadrupole time-of-flight mass spectroscopy;UHPLC-QTOF-MS) of hemolymph from two freshwater mussel species (Lampsilis cardium and L. siliquoidea) from three streams in Indiana. Concentrations of metabolites were modeled by species, stream and sex to determine the influence of each parameter.
Samples in Transit
John Steiner preparing to bring wastewater samples to FedEx for shipping.
Determination of antimycin–a in a liquid formulation by high performance liquid chromatography–mass spectrometry
Pesticide formulations containing the active ingredient antimycin–a (ANT–A) have been used by fisheries and aquaculture managers for several decades to remove nuisance fish species. Analytical methods for measuring ANT–A during pesticide treatments have been done using high performance liquid chromatography (HPLC) paired with multiple detection methods (for example, electrochemical, ultraviolet, f
Authors
Gavin N. Saari, J. Nolan Steiner, Bryan Lada, Nadia Carmosini
A novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp
Invasive carp pose substantial economic and ecological damage when populations are widespread in freshwater systems within the United States. Resource managers in the United States have few chemical control tools to selectively remove nuisance fish. This study examined whether Antimycin–A (antimycin) wax encapsulated microparticles could cause selective lethality in invasive carps. The antimycin m
Authors
Blake W. Sauey, Gavin N. Saari, Joel G. Putnam, Justine E. Nelson, James J. Wamboldt, J. Nolan Steiner, Robin D. Calfee
Toxicity of a management bait for grass carp (Ctenopharyngodon idella) incorporated with Antimycin A
No current technology can specifically target grass carp (Ctenopharyngodon idella) for control within aquatic ecosystems. Rotenone and Carbon Dioxide-Carp are currently the only available registered pesticides for grass carp; they are nonselective and typically applied throughout the water, equally exposing target and native species. A more selective control tool or pesticide application could be
Authors
James J. Wamboldt, J. Nolan Steiner, Blake W. Sauey, Bryan Lada, Joel G. Putnam, Brianne Marjorie Korducki, Gavin N. Saari
Mussel mass mortality in the Clinch River, USA: Metabolomics detects affected pathways and biomarkers of stress
Biologists monitoring freshwater mussel (order Unionida) populations rely on behavioral, often subjective, signs to identify moribund (“sick”) or stressed mussels, such as gaping valves and slow response to probing, and they lack clinical indicators to support a diagnosis. As part of a multi-year study to investigate causes of reoccurring mortality of pheasantshell (Ortmanniana pectorosa; synonym
Authors
Joel G. Putnam, J. Nolan Steiner, Jordon Richard, Eric Leis, Tony Goldberg, Christopher D. Dunn, Rose Agbalog, Susan Knowles, Diane L. Waller
Targeted metabolomics characterizes metabolite occurrence and variability in stable freshwater mussel populations
Freshwater mussels (order Unionida) play a key role in freshwater systems as ecosystem engineers and indicators of aquatic ecosystem health. The fauna is globally imperilled due to a diversity of suspected factors; however, causes for many population declines and mortality events remain unconfirmed due partly to limited health assessment tools. Mussel-monitoring activities often rely on population
Authors
Diane L. Waller, Joel Putnam, J. Nolan Steiner, Brant Fisher, Grant N. Burcham, John W. Oliver, Stephen B. Smith, Richard A. Erickson, Anne Remek, Nancy Bodeker
Quantitative method development to determine feed consumption using a dye
Although there are many methods to determine ingestion and absorption of aquafeeds, none exist that are simple, cost-effective, and quantitative and that can mark fish with a long-lasting, visible indicator. In addition to aquafeed development, selective baits are needed that can be used for aquatic invasive species removal efforts, including for Grass Carp Ctenopharyngodon idella. Bait incorporat
Authors
James J. Wamboldt, Justine Nelson, Linnea M Thomas, J. Nolan Steiner, Jillian Hebert, Richard A. Erickson, Joel G. Putnam
Science and Products
Data release for determination of antimycin–a in liquid formulation by high performance liquid chromatography–mass spectrometry
Pesticide formulations containing the active ingredient Antimycin-A (ANT-A) have been used by fisheries and aquaculture managers for several decades to remove nuisance fish species. Analytical methods for measuring ANT-A during pesticide treatments have been reported using High Performance Liquid Chromatography (HPLC) paired with multiple detection methods (e.g., electrochemical, ultraviolet, fluo
Data release for toxicity of Antimycin A incorporated management bait for grass carp
The goal of this study was to develop and examine whether a management bait that can be used for selective control of grass carp. Our objectives were to 1) quantify the water-based 24-h LC50 of Antimycin-A for grass carp and rainbow trout, 2) quantify the 96-h LD50 of orally administered Antimycin-A laden bait for grass carp and rainbow trout, 3) quantify the leaching rate of Antimycin-A from the
Metabolomic analysis of pheasantshell mussel (Ortmanniana pectorosa; Order Unionida) from a mass mortality event in the Clinch River, Virginia and Tennessee, USA
This dataset uses the ITIS recommended Ortmanniana pectorosa, Conrad, 1834 in place of Actinonaias pectorosa, which was used in the Richard et al. 2020 manuscript. Biologists monitoring freshwater mussel (Order Unionida) populations rely on behavioral, often subjective, symptoms to identify “sick” or stressed animals, such as gaping valves and slow response to probing and lack clinical indicators
Characterization of occurrence and abundance of targeted metabolites in unionid mussels from three streams
The data consists of targeted metabolite analysis (using quadrupole time-of-flight mass spectroscopy;UHPLC-QTOF-MS) of hemolymph from two freshwater mussel species (Lampsilis cardium and L. siliquoidea) from three streams in Indiana. Concentrations of metabolites were modeled by species, stream and sex to determine the influence of each parameter.
Samples in Transit
John Steiner preparing to bring wastewater samples to FedEx for shipping.
John Steiner preparing to bring wastewater samples to FedEx for shipping.
Determination of antimycin–a in a liquid formulation by high performance liquid chromatography–mass spectrometry
Pesticide formulations containing the active ingredient antimycin–a (ANT–A) have been used by fisheries and aquaculture managers for several decades to remove nuisance fish species. Analytical methods for measuring ANT–A during pesticide treatments have been done using high performance liquid chromatography (HPLC) paired with multiple detection methods (for example, electrochemical, ultraviolet, f
Authors
Gavin N. Saari, J. Nolan Steiner, Bryan Lada, Nadia Carmosini
A novel tool to selectively deliver a control agent to filter-feeding silver and bighead carp
Invasive carp pose substantial economic and ecological damage when populations are widespread in freshwater systems within the United States. Resource managers in the United States have few chemical control tools to selectively remove nuisance fish. This study examined whether Antimycin–A (antimycin) wax encapsulated microparticles could cause selective lethality in invasive carps. The antimycin m
Authors
Blake W. Sauey, Gavin N. Saari, Joel G. Putnam, Justine E. Nelson, James J. Wamboldt, J. Nolan Steiner, Robin D. Calfee
Toxicity of a management bait for grass carp (Ctenopharyngodon idella) incorporated with Antimycin A
No current technology can specifically target grass carp (Ctenopharyngodon idella) for control within aquatic ecosystems. Rotenone and Carbon Dioxide-Carp are currently the only available registered pesticides for grass carp; they are nonselective and typically applied throughout the water, equally exposing target and native species. A more selective control tool or pesticide application could be
Authors
James J. Wamboldt, J. Nolan Steiner, Blake W. Sauey, Bryan Lada, Joel G. Putnam, Brianne Marjorie Korducki, Gavin N. Saari
Mussel mass mortality in the Clinch River, USA: Metabolomics detects affected pathways and biomarkers of stress
Biologists monitoring freshwater mussel (order Unionida) populations rely on behavioral, often subjective, signs to identify moribund (“sick”) or stressed mussels, such as gaping valves and slow response to probing, and they lack clinical indicators to support a diagnosis. As part of a multi-year study to investigate causes of reoccurring mortality of pheasantshell (Ortmanniana pectorosa; synonym
Authors
Joel G. Putnam, J. Nolan Steiner, Jordon Richard, Eric Leis, Tony Goldberg, Christopher D. Dunn, Rose Agbalog, Susan Knowles, Diane L. Waller
Targeted metabolomics characterizes metabolite occurrence and variability in stable freshwater mussel populations
Freshwater mussels (order Unionida) play a key role in freshwater systems as ecosystem engineers and indicators of aquatic ecosystem health. The fauna is globally imperilled due to a diversity of suspected factors; however, causes for many population declines and mortality events remain unconfirmed due partly to limited health assessment tools. Mussel-monitoring activities often rely on population
Authors
Diane L. Waller, Joel Putnam, J. Nolan Steiner, Brant Fisher, Grant N. Burcham, John W. Oliver, Stephen B. Smith, Richard A. Erickson, Anne Remek, Nancy Bodeker
Quantitative method development to determine feed consumption using a dye
Although there are many methods to determine ingestion and absorption of aquafeeds, none exist that are simple, cost-effective, and quantitative and that can mark fish with a long-lasting, visible indicator. In addition to aquafeed development, selective baits are needed that can be used for aquatic invasive species removal efforts, including for Grass Carp Ctenopharyngodon idella. Bait incorporat
Authors
James J. Wamboldt, Justine Nelson, Linnea M Thomas, J. Nolan Steiner, Jillian Hebert, Richard A. Erickson, Joel G. Putnam