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Enteroctopus Genetic Data Evaluating DNA Sampling Technique Effects on Data Integrity

June 30, 2017

These are genetic data collected from Enteroctopus (n = 21; E. dofleini or a cryptic lineage) to evaluate DNA yields obtained via invasive or destructive sampling (muscle/arm tips) versus non-invasive sampling (epithelial swabs) and effects on data reliability as well as to assess the accuracy of using two microsatellite markers as a rapid screen to identify the two Enteroctopus lineages. Paired samples of both epithelial swabs and muscle (arm tip) were obtained from the same individuals caught as by-catch during commercial shrimp fishing and other surveys of western Prince William Sound, Alaska during autumn 2012/2013 and spring 2013. Treatment groups for the paired samples included epithelial swabs placed in a commonly used DNA sampling buffer and muscle samples placed in ethanol stored at ambient temperature until DNA extraction occurred. Initial DNA extractions commenced within one day of receipt at the laboratory. A second DNA extraction was performed on epithelial swabs 19-31 months post-collection to re-evaluate their suitability as a source for DNA (quantity and reliability). Data collected from all samples include two microsatellites identified as possible lineage indicators (EdoµA129 and EdoµA106), and nine microsatellite loci previously identifed as polymorphic in both lineages (EdoµA6, A8, A105, A110, A118, A122, A125, A129 and E11). DNA sequence data from 528 bp of the octopine dehydrogenase (OCDE) gene were collected from muscle extractions for all samples and from a sub-set of epithelial extractions.

Publication Year 2017
Title Enteroctopus Genetic Data Evaluating DNA Sampling Technique Effects on Data Integrity
DOI 10.5066/F7VM49HJ
Authors Sandra L Talbot
Product Type Data Release
Record Source USGS Digital Object Identifier Catalog
USGS Organization Alaska Science Center