Genetic Data Used to Develop a Genotyping-in-Thousands by Sequencing (GT-seq) Panel for Identifying Individuals and Estimating Relatedness in Alaska Black Bears (Ursus americanus)
April 14, 2025
This data package includes genotype data for two independent sets of American black bear (Ursus americanus) samples. The first set of samples was collected from 85 adult bears harvested by permitted hunters in southcentral Alaska from 1994-2019; we hereafter refer to these as "hunter-harvested" samples. These hunter-harvested samples were genotyped with restriction site-associated sequencing (RAD-seq) and were used to detect sequence variants (microhaplotypes and SNPs) with high heterozygosity in Alaska bear populations, and design primers for 'genotyping-in-thousands by sequencing' (GT-seq). The second set of samples includes 79 of the hunter-harvested samples, as well as 84 additional samples collected from adult and juvenile bears on the Joint Base Elmendorf-Richardson (JBER) in Anchorage, Alaska, from 2020 to 2022. These samples were genotyped at 170 microhaplotype loci using GT-seq.
Citation Information
Publication Year | 2025 |
---|---|
Title | Genetic Data Used to Develop a Genotyping-in-Thousands by Sequencing (GT-seq) Panel for Identifying Individuals and Estimating Relatedness in Alaska Black Bears (Ursus americanus) |
DOI | 10.5066/P1DIDUPX |
Authors | Eleni L Petrou, Colette D Brandt, Sean D Farley, Timothy J Spivey, Kristen Gruenthal, Cory Stantorf, David Battle, Cherie M Mckeeman, Andy M Ramey |
Product Type | Data Release |
Record Source | USGS Asset Identifier Service (AIS) |
USGS Organization | Alaska Science Center |
Rights | This work is marked with CC0 1.0 Universal |
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Development of a genotyping-in-thousands by sequencing (GT-seq) panel for identifying individuals and estimating relatedness among Alaska black bears (Ursus americanus)
The management and conservation of large mammals, such as black bears (Ursus americanus), have long been informed by genetic estimates of population size and individual dispersal. Amplicon sequencing methods, also known as ‘genotyping-in-thousands-by sequencing’ (GT-seq), now enable the efficient and cost-effective genotyping of hundreds of loci and individuals in the same sequencing run...
Authors
Ethan Petrou, Colette D. Brandt, Timothy J. Spivey, Kristen M. Gruenthal, Cherie Marie McKeeman, Sean D. Farley, David Battle, Cory Stantorf, Andrew M. Ramey
Related
Development of a genotyping-in-thousands by sequencing (GT-seq) panel for identifying individuals and estimating relatedness among Alaska black bears (Ursus americanus)
The management and conservation of large mammals, such as black bears (Ursus americanus), have long been informed by genetic estimates of population size and individual dispersal. Amplicon sequencing methods, also known as ‘genotyping-in-thousands-by sequencing’ (GT-seq), now enable the efficient and cost-effective genotyping of hundreds of loci and individuals in the same sequencing run...
Authors
Ethan Petrou, Colette D. Brandt, Timothy J. Spivey, Kristen M. Gruenthal, Cherie Marie McKeeman, Sean D. Farley, David Battle, Cory Stantorf, Andrew M. Ramey