This data set includes two tables associated with a study of Plasmodium parasite infection in mosquitoes collected in south-central Alaska during the summer of 2016. The first table contains data regarding mosquito collection from three sites using CO2 baited CDC light traps. Mosquitoes were sorted and pooled according to collection date, genus, and location and subsequently screened for Plasmodium parasite infection using molecular methods. The second table contains results of Plasmodium parasite screening of pooled mosquito abdomen and head/thorax segments using PCR. Parasite genetic data is provided as GenBank accession numbers for any samples that tested positive for Plasmodium infection.