These data were collected during a travel time and dissolved oxygen study along a 1-mile reach of the Blue River downstream from 58th Street in Kansas City, Missouri. The study was a cooperative effort between the U.S. Geological Survey (USGS), the U.S. Environmental Protection Agency Urban Waters Federal Partnership program, and the Kansas City, Missouri, Water Services Department. This is part of a larger USGS effort supporting the Middle Blue River Basin Urban Waters Federal Partnership. This data release contains continuous water-quality measurements (Blue_River_CQWM.csv), rhodamine dye sensor data (Blue_River_dye_profiles.csv), analytical data from synoptic water-quality samples (Blue_River_QWDATA.csv), manual field measurements (Blue_River_manual_measurements.csv), and station information and comments for sensor data (Blue_River_stations.csv).