Fig. 2. Nonlinear regression prediction plot for use with 50 pooled l

Fig. 2.  Nonlinear regression prediction plot for use with 50 pooled larvae

Detailed Description

Fig. 2. Nonlinear regression prediction plot for use with 50 pooled larvae that accurately estimates the percentage of triploids in a mixed population of triploids and diploids of that spawn. The plot was generated from 10 replicate experiments, each using a FCM protocol for cell processing and analysis at 15 known levels of ploidy. The 95% confidence limits (black dashed lines) and mean values (dots), along with the 95% prediction curves (red dashed lines) are shown. The corresponding quadratic equation is above. Thus, a flow cytometric-derived observed value (y–axis) (within the range) will correspond to a predicted triploidy value (x-axis) for a spawn.

Details

Image Dimensions: 495 x 609