Skip to main content
U.S. flag

An official website of the United States government

Evaluation of a Chicken 600K SNP genotyping array in non-model species of grouse

April 23, 2019

The use of single nucleotide polymorphism (SNP) arrays to generate large SNP datasets for comparison purposes have recently become an attractive alternative to other genotyping methods. Although most SNP arrays were originally developed for domestic organisms, they can be effectively applied to wild relatives to obtain large panels of SNPs. In this study, we tested the cross-species application of the Affymetrix 600K Chicken SNP array in five species of North American prairie grouse (Centrocercus and Tympanuchus genera). Two individuals were genotyped per species for a total of ten samples. A high proportion (91%) of the total 580 961 SNPs were genotyped in at least one individual (73–76% SNPs genotyped per species). Principal component analysis with autosomal SNPs separated the two genera, but failed to clearly distinguish species within genera. Gene ontology analysis identified a set of genes related to morphogenesis and development (including genes involved in feather development), which may be primarily responsible for large phenotypic differences between Centrocercus and Tympanuchus grouse. Our study provided evidence for successful cross-species application of the chicken SNP array in grouse which diverged ca. 37 mya from the chicken lineage. As far as we are aware, this is the first reported application of a SNP array in non-passerine birds, and it demonstrates the feasibility of using commercial SNP arrays in research on non-model bird species.

Publication Year 2019
Title Evaluation of a Chicken 600K SNP genotyping array in non-model species of grouse
DOI 10.1038/s41598-019-42885-5
Authors Piotr Minias, Peter O. Dunn, Linda A. Whittingham, Jeff A. Johnson, Sara J. Oyler-McCance
Publication Type Article
Publication Subtype Journal Article
Series Title Scientific Reports
Index ID 70215103
Record Source USGS Publications Warehouse
USGS Organization Fort Collins Science Center