Field sampling guidelines for developing and verifying satellite remote sensing chlorophyll a concentration and fluorescence models in inland waters

Harmful algal blooms are increasing in frequency in inland waters across the United States, resulting in a need to monitor phytoplankton bloom events to track ecosystem health and productivity. Remote sensing of chlorophyll a values offers a cost-effective and powerful method for early detection and characterization of bloom events and serves as an overall indicator of water quality and trophic state, with regular, repeated sampling of landscape-wide, high spatial resolution measurements. Field measurements are necessary for developing and verifying chlorophyll a retrieval models. For model verification, chlorophyll a concentration or fluorescence and light attenuation measurements are needed; for model development, turbidity and colored dissolved organic matter concentration measurements are additionally needed; and for model development and verification, radiometric measurements, taxonomic identification of phytoplankton, inherent optical properties, and cyanotoxin concentration are further measurements that can provide context. This report outlines detailed methods and priority considerations for collecting high-quality field data in inland waters (defined as rivers, lakes, reservoirs, estuaries, streams, and wetlands). The described methods include best practices for collecting and preparing discretely collected water samples and for calibration, maintenance, and quality assurance and quality control of field sensors. Whereas the priorities will vary between applications, some general guidelines are to collect field samples (1) as close in time to a satellite overpass as possible, (2) from representative areas of the waterbody to capture the range of spatial variability, and (3) near the surface to match remote sensing reflectance data.