Suspended-sediment-concentration (SSC) regimes of five biological reference streams in Tennessee were characterized from 15-minute SSC records spanning 1 to 4 water years (October 1 through September 30) between 2004 and 2008. These streams represent least disturbed conditions for their respective ecoregions and have exceptional biodiversity in terms of fish or aquatic invertebrates. SSC regimes in streams, when plotted in terms of duration above a given SSC at a given annual frequency such as the annual maximum or the annual tenth longest duration, can be compared directly to published biological impairment thresholds derived from experimental trials. Based on such comparison, the SSC regimes of all five reference streams reached published impairment thresholds at least 10 times per water year for all years of record. The results suggest that the published impairment thresholds are not directly applicable to streams in Tennessee and, by extension, the southeastern United States.