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Uranium quantification in semen by inductively coupled plasma mass spectrometry

June 12, 2013

In this study we report uranium analysis for human semen samples. Uranium quantification was performed by inductively coupled plasma mass spectrometry. No additives, such as chymotrypsin or bovine serum albumin, were used for semen liquefaction, as they showed significant uranium content. For method validation we spiked 2 g aliquots of pooled control semen at three different levels of uranium: low at 5 pg/g, medium at 50 pg/g, and high at 1000 pg/g. The detection limit was determined to be 0.8 pg/g uranium in human semen. The data reproduced within 1.4–7% RSD and spike recoveries were 97–100%. The uranium level of the unspiked, pooled control semen was 2.9 pg/g of semen (n = 10). In addition six semen samples from a cohort of Veterans exposed to depleted uranium (DU) in the 1991 Gulf War were analyzed with no knowledge of their exposure history. Uranium levels in the Veterans’ semen samples ranged from undetectable (<0.8 pg/g) to 3350 pg/g. This wide concentration range for uranium in semen is consistent with known differences in current DU body burdens in these individuals, some of whom have retained embedded DU fragments.

Citation Information

Publication Year 2013
Title Uranium quantification in semen by inductively coupled plasma mass spectrometry
DOI 10.1016/j.jtemb.2012.07.004
Authors Todor I. Todorov, John W. Ejnik, Gustavo S. Guandalini, Hanna Xu, Dennis Hoover, Larry W. Anderson, Katherine Squibb, Melissa A. McDiarmid, Jose A. Centeno
Publication Type Article
Publication Subtype Journal Article
Series Title Journal of Trace Elements in Medicine and Biology
Index ID 70189065
Record Source USGS Publications Warehouse
USGS Organization Crustal Geophysics and Geochemistry Science Center