From water to web: Trophic transfer of neonicotinoids from a wastewater effluent-dominated stream to riparian spiders

Municipal wastewater is a known point source of organic contaminants, including pharmaceuticals and neonicotinoid insecticides. Emergent aquatic insects can provide a direct aquatic-to-terrestrial contaminant transfer route to the food web, with implications for terrestrial food web dispersal of wastewater-derived organic contaminants. We quantified 17 target pharmaceuticals and insecticides (log K_ow: −1.43 to 4.75) in surface water, fish, aquatic insects, and web-building riparian spiders at a wastewater effluent-dominated stream in eastern Iowa, USA. Two neonicotinoids, imidacloprid and clothianidin, had spider tissue concentrations of 8.9–84 ng/g and 1.2–11 ng/g, respectively. The imidacloprid/clothianidin ratios in spider tissues were reflective of the concentration ratios in the effluent-dominated streamwater and opposite of nearby agriculturally dominated waters. In contrast, no pharmaceuticals were detectable in the riparian spiders; however, only pharmaceuticals were present in both fish and aquatic insects (1.1–11 ng/g and 5.9–51 ng/g, respectively). Neonicotinoids are not predicted to enter aquatic food webs based on their log K_ow and bioconcentration factor values; therefore, an implication of this study is to warrant caution when using traditional bioaccumulation models for polar hydrophilic contaminants. This work provides further evidence that neonicotinoids undergo trophic transfer and represents the initial measurements, implicating such a transfer from effluent-dominated streams into terrestrial food webs. While this study emphasizes field-relevant observations, it is limited by environmental variability, including uncertainties in the biomass of emergent insects that likely contribute to spider diets. Future research could investigate contaminant metabolites within individual organisms or use complementary techniques to better understand the underlying mechanisms.