Jon Amberg, PhD
Jon Amberg is the Center Director for the Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin.
Science and Products
Filter Total Items: 14
Use of antimycin-microparticles and alfalfa to control Grass Carp Ctenopharyngodon idella
Since their introduction in the 1960’s, grass carp ( Ctenopharyngodon idella) have spread across the North American continent as far north as the U.S./Canadian border in the Great Lakes. Grass carp are an herbivorous species and can significantly reduce the amount of macrophytes and other plant material in a body of water. Subsequently, this can lead to many detrimental effects for certain species...
Hunting Invasive Species with HTCondor: High Throughput Computing for Big Data and Next Generation Sequencing
Large amounts of data are being generated that require hours, days, or even weeks to analyze using traditional computing resources. Innovative solutions must be implemented to analyze the data in a reasonable timeframe. The program HTCondor (https://research.cs.wisc.edu/htcondor/) takes advantage of the processing capacity of individual desktop computers and dedicated computing resources...
Filter Total Items: 53
Development of a quantitative PCR method for screening ichthyoplankton samples for bigheaded carps Development of a quantitative PCR method for screening ichthyoplankton samples for bigheaded carps
Monitoring ichthyoplankton is useful for identifying reproductive fronts and spawning locations of bigheaded carps (Hypophthalmichthys spp.). Unfortunately, sorting and identifying ichthyoplankton to monitor for bigheaded carp reproduction is time consuming and expensive. Traditional methods require frequent egg-larvae sampling, sorting of all samples to obtain presumptively identified...
Authors
Andrea K. Fritts, Brent C. Knights, James H. Larson, Jon Amberg, Christopher M. Merkes, Tariq Tajjioui, Steven E. Butler, Matthew J. Diana, David H. Wahl, Michael J. Weber, John D. Waters
Environmental DNA as a tool to help inform zebra mussel, Dreissena polymorpha, management in inland lakes Environmental DNA as a tool to help inform zebra mussel, Dreissena polymorpha, management in inland lakes
Zebra mussels (Dreissena polymorpha) are an aquatic invasive species that plague much of North America and are difficult to impossible to eradicate once they become established. Therefore, prevention and monitoring are key elements in the control of these organisms. Traditional microscopy is commonly used in monitoring but requires the presence of larval veligers. This limits the times...
Authors
Jon Amberg, Christopher M. Merkes, Wendylee Stott, Christopher B. Rees, Richard A. Erickson
Correlating sea lamprey density with environmental DNA detections in the lab Correlating sea lamprey density with environmental DNA detections in the lab
Invasive sea lamprey (Petromyzon marinus Linnaeus, 1758) are currently managed by the Great Lakes Fishery Commission in an effort to reduce pest populations below levels that cause ecological damage. One technique to improve stream population assessments could be molecular surveillance in the form of environmental DNA (eDNA) monitoring. We developed and validated four probe-based...
Authors
Nicholas A. Schloesser, Christopher M. Merkes, Christopher B. Rees, Jon Amberg, Todd B. Steeves, Margaret F. Docker
Field evaluation of carbon dioxide as a fish deterrent at a water management structure along the Illinois River Field evaluation of carbon dioxide as a fish deterrent at a water management structure along the Illinois River
Construction of a water management structure (WMS) in the levee surrounding The Nature Conservancy’s Emiquon Preserve (Havana, Illinois, USA) created a new hydrological connection and potential aquatic invasive species pathway between the Illinois River and a large conservation wetland complex. Site managers need a control tool that deters the upstream passage of non-native fishes into...
Authors
Aaron R. Cupp, Justin R. Smerud, John Tix, Susan M. Schleis, Kim T. Fredricks, Richard A. Erickson, Jon Amberg, William S. Morrow, Carolyn M. Koebel, Elizabeth A. Murphy, Chad Vishy, K. Douglas Blodgett
The complete mitochondrial genome of Hine’s emerald dragonfly (Somatochlora hineana Williamson) via NGS sequencing The complete mitochondrial genome of Hine’s emerald dragonfly (Somatochlora hineana Williamson) via NGS sequencing
Here, we report the complete mitochondrial genome of the endangered Hine’s emerald dragonfly (HED), Somatochlora hineana Williamson. Data were generated via next generation sequencing (NGS) and assembled using a mitochondrial baiting and iterative mapping approach. The full length circular genome is 15,705 bp with 26.6% GC content. It contains the typical metazoan set of 37 genes: 13...
Authors
Craig Jackson, S. Grace McCalla, Jon Amberg, Daniel Soluk, Hugh Britten
Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA
Environmental DNA (eDNA) samples that are collected from remote locations depend on rapid stabilization of the DNA. The degradation of eDNA in water samples is minimized when samples are stored at ≤ 4 °C. Developing a preservation technique to maintain eDNA integrity at room temperature would allow a wider range of locations to be sampled. We evaluated an ethanol and sodium acetate...
Authors
Bridget A. Ladell, Liza R. Walleser, S. Grace McCalla, Richard A. Erickson, Jon Amberg
Science and Products
Filter Total Items: 14
Use of antimycin-microparticles and alfalfa to control Grass Carp Ctenopharyngodon idella
Since their introduction in the 1960’s, grass carp ( Ctenopharyngodon idella) have spread across the North American continent as far north as the U.S./Canadian border in the Great Lakes. Grass carp are an herbivorous species and can significantly reduce the amount of macrophytes and other plant material in a body of water. Subsequently, this can lead to many detrimental effects for certain species...
Hunting Invasive Species with HTCondor: High Throughput Computing for Big Data and Next Generation Sequencing
Large amounts of data are being generated that require hours, days, or even weeks to analyze using traditional computing resources. Innovative solutions must be implemented to analyze the data in a reasonable timeframe. The program HTCondor (https://research.cs.wisc.edu/htcondor/) takes advantage of the processing capacity of individual desktop computers and dedicated computing resources...
Filter Total Items: 53
Development of a quantitative PCR method for screening ichthyoplankton samples for bigheaded carps Development of a quantitative PCR method for screening ichthyoplankton samples for bigheaded carps
Monitoring ichthyoplankton is useful for identifying reproductive fronts and spawning locations of bigheaded carps (Hypophthalmichthys spp.). Unfortunately, sorting and identifying ichthyoplankton to monitor for bigheaded carp reproduction is time consuming and expensive. Traditional methods require frequent egg-larvae sampling, sorting of all samples to obtain presumptively identified...
Authors
Andrea K. Fritts, Brent C. Knights, James H. Larson, Jon Amberg, Christopher M. Merkes, Tariq Tajjioui, Steven E. Butler, Matthew J. Diana, David H. Wahl, Michael J. Weber, John D. Waters
Environmental DNA as a tool to help inform zebra mussel, Dreissena polymorpha, management in inland lakes Environmental DNA as a tool to help inform zebra mussel, Dreissena polymorpha, management in inland lakes
Zebra mussels (Dreissena polymorpha) are an aquatic invasive species that plague much of North America and are difficult to impossible to eradicate once they become established. Therefore, prevention and monitoring are key elements in the control of these organisms. Traditional microscopy is commonly used in monitoring but requires the presence of larval veligers. This limits the times...
Authors
Jon Amberg, Christopher M. Merkes, Wendylee Stott, Christopher B. Rees, Richard A. Erickson
Correlating sea lamprey density with environmental DNA detections in the lab Correlating sea lamprey density with environmental DNA detections in the lab
Invasive sea lamprey (Petromyzon marinus Linnaeus, 1758) are currently managed by the Great Lakes Fishery Commission in an effort to reduce pest populations below levels that cause ecological damage. One technique to improve stream population assessments could be molecular surveillance in the form of environmental DNA (eDNA) monitoring. We developed and validated four probe-based...
Authors
Nicholas A. Schloesser, Christopher M. Merkes, Christopher B. Rees, Jon Amberg, Todd B. Steeves, Margaret F. Docker
Field evaluation of carbon dioxide as a fish deterrent at a water management structure along the Illinois River Field evaluation of carbon dioxide as a fish deterrent at a water management structure along the Illinois River
Construction of a water management structure (WMS) in the levee surrounding The Nature Conservancy’s Emiquon Preserve (Havana, Illinois, USA) created a new hydrological connection and potential aquatic invasive species pathway between the Illinois River and a large conservation wetland complex. Site managers need a control tool that deters the upstream passage of non-native fishes into...
Authors
Aaron R. Cupp, Justin R. Smerud, John Tix, Susan M. Schleis, Kim T. Fredricks, Richard A. Erickson, Jon Amberg, William S. Morrow, Carolyn M. Koebel, Elizabeth A. Murphy, Chad Vishy, K. Douglas Blodgett
The complete mitochondrial genome of Hine’s emerald dragonfly (Somatochlora hineana Williamson) via NGS sequencing The complete mitochondrial genome of Hine’s emerald dragonfly (Somatochlora hineana Williamson) via NGS sequencing
Here, we report the complete mitochondrial genome of the endangered Hine’s emerald dragonfly (HED), Somatochlora hineana Williamson. Data were generated via next generation sequencing (NGS) and assembled using a mitochondrial baiting and iterative mapping approach. The full length circular genome is 15,705 bp with 26.6% GC content. It contains the typical metazoan set of 37 genes: 13...
Authors
Craig Jackson, S. Grace McCalla, Jon Amberg, Daniel Soluk, Hugh Britten
Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA Ethanol and sodium acetate as a preservation method to delay degradation of environmental DNA
Environmental DNA (eDNA) samples that are collected from remote locations depend on rapid stabilization of the DNA. The degradation of eDNA in water samples is minimized when samples are stored at ≤ 4 °C. Developing a preservation technique to maintain eDNA integrity at room temperature would allow a wider range of locations to be sampled. We evaluated an ethanol and sodium acetate...
Authors
Bridget A. Ladell, Liza R. Walleser, S. Grace McCalla, Richard A. Erickson, Jon Amberg