Jon Amberg, PhD
Jon Amberg is the Center Director for the Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin.
Science and Products
Filter Total Items: 14
Filter Total Items: 53
Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites
The performance of newly developed New Zealand mudsnail (Potamopyrgus antipodarum; NZMS) genetic markers for environmental (eDNA) analysis of water were compared across two laboratories. The genetic markers were tested in four quantitative polymerase chain reaction assays targeting two regions of the NZMS mitochondrial genome, specifically the cytochrome c oxidase subunit 1 (coi) and...
Authors
Christopher M. Merkes, Keith N. Turnquist, Christopher B. Rees, Jon J. Amberg
Environmental DNA calibration study. Interim technical review report Environmental DNA calibration study. Interim technical review report
Invasive aquatic nuisance species pose a major threat to aquatic ecosystems worldwide. Invasive Asian carps, including bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix) have been steadily dispersing upstream through the Mississippi, Illinois, and Des Plaines Rivers since the 1990s. To prevent further movement up the Illinois River into the Chicago Area Waterway...
Authors
K. Baerwaldt, Meredith L. Bartron, K. Schilling, Debbie Lee, Edmond Russo, Trudy Estes, Richard Fischer, Beth Fleming, Michael P. Guilfoyle, K. Jack Kilgore, Richard Lance, Edward Perkins, Martin Schultz, David Smith, Jon J. Amberg, Duane Chapman, Mark P. Gaikowski, Katy E. Klymus, Cathy A. Richter
Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data
The prevention of non-indigenous aquatic invasive species spreading into new areas is a goal of many resource managers. New techniques have been developed to survey for species that are difficult to capture with conventional gears that involve the detection of their DNA in water samples (eDNA). This technique is currently used to track the invasion of bigheaded carps (silver carp and...
Authors
Christopher M. Merkes, S. Grace McCalla, Nathan R. Jensen, Mark P. Gaikowski, Jon J. Amberg
Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system
To better understand potential diet overlap among exotic Asian species of carp and native species of filter-feeding fishes of the upper Mississippi River system, microscopy was used to document morphological differences in the gill rakers. Analysing samples first with light microscopy and subsequently with confocal microscopy, the three-dimensional structure of gill rakers in...
Authors
Liza R. Walleser, D.R. Howard, Mark B. Sandheinrich, Mark P. Gaikowski, Jon J. Amberg
Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers
Improved management of invasive Silver Carp Hypophthalmichthys molitrix in the upper Mississippi River basin may be possible by better understanding the feeding abilities of this population. Food collection for filter-feeding fishes, such as Silver Carp, is influenced by the species-specific structure of their gill rakers. To investigate structural variation in gill rakers of Silver Carp...
Authors
Liza R. Walleser, Mark B. Sandheinrich, David R. Howard, Mark P. Gaikowski, Jon J. Amberg
Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish
Gut microbiota of invasive Asian silver carp (SVCP) and indigenous planktivorous gizzard shad (GZSD) in Mississippi river basin were compared using 16S rRNA gene pyrosequencing. Analysis of more than 440 000 quality-filtered sequences obtained from the foregut and hindgut of GZSD and SVCP revealed high microbial diversity in these samples. GZSD hindgut (GZSD_H) samples (n=23) with >7000
Authors
Lin Ye, Jon J. Amberg, Duane Chapman, Mark P. Gaikowski, Wen-Tso Liu
Science and Products
Filter Total Items: 14
Filter Total Items: 53
Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites
The performance of newly developed New Zealand mudsnail (Potamopyrgus antipodarum; NZMS) genetic markers for environmental (eDNA) analysis of water were compared across two laboratories. The genetic markers were tested in four quantitative polymerase chain reaction assays targeting two regions of the NZMS mitochondrial genome, specifically the cytochrome c oxidase subunit 1 (coi) and...
Authors
Christopher M. Merkes, Keith N. Turnquist, Christopher B. Rees, Jon J. Amberg
Environmental DNA calibration study. Interim technical review report Environmental DNA calibration study. Interim technical review report
Invasive aquatic nuisance species pose a major threat to aquatic ecosystems worldwide. Invasive Asian carps, including bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix) have been steadily dispersing upstream through the Mississippi, Illinois, and Des Plaines Rivers since the 1990s. To prevent further movement up the Illinois River into the Chicago Area Waterway...
Authors
K. Baerwaldt, Meredith L. Bartron, K. Schilling, Debbie Lee, Edmond Russo, Trudy Estes, Richard Fischer, Beth Fleming, Michael P. Guilfoyle, K. Jack Kilgore, Richard Lance, Edward Perkins, Martin Schultz, David Smith, Jon J. Amberg, Duane Chapman, Mark P. Gaikowski, Katy E. Klymus, Cathy A. Richter
Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data
The prevention of non-indigenous aquatic invasive species spreading into new areas is a goal of many resource managers. New techniques have been developed to survey for species that are difficult to capture with conventional gears that involve the detection of their DNA in water samples (eDNA). This technique is currently used to track the invasion of bigheaded carps (silver carp and...
Authors
Christopher M. Merkes, S. Grace McCalla, Nathan R. Jensen, Mark P. Gaikowski, Jon J. Amberg
Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system
To better understand potential diet overlap among exotic Asian species of carp and native species of filter-feeding fishes of the upper Mississippi River system, microscopy was used to document morphological differences in the gill rakers. Analysing samples first with light microscopy and subsequently with confocal microscopy, the three-dimensional structure of gill rakers in...
Authors
Liza R. Walleser, D.R. Howard, Mark B. Sandheinrich, Mark P. Gaikowski, Jon J. Amberg
Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers
Improved management of invasive Silver Carp Hypophthalmichthys molitrix in the upper Mississippi River basin may be possible by better understanding the feeding abilities of this population. Food collection for filter-feeding fishes, such as Silver Carp, is influenced by the species-specific structure of their gill rakers. To investigate structural variation in gill rakers of Silver Carp...
Authors
Liza R. Walleser, Mark B. Sandheinrich, David R. Howard, Mark P. Gaikowski, Jon J. Amberg
Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish
Gut microbiota of invasive Asian silver carp (SVCP) and indigenous planktivorous gizzard shad (GZSD) in Mississippi river basin were compared using 16S rRNA gene pyrosequencing. Analysis of more than 440 000 quality-filtered sequences obtained from the foregut and hindgut of GZSD and SVCP revealed high microbial diversity in these samples. GZSD hindgut (GZSD_H) samples (n=23) with >7000
Authors
Lin Ye, Jon J. Amberg, Duane Chapman, Mark P. Gaikowski, Wen-Tso Liu