Jon Amberg, PhD
Jon Amberg is the Center Director for the Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin.
Science and Products
Filter Total Items: 14
Filter Total Items: 53
Responses of invasive silver and bighead carp to a carbon dioxide barrier in outdoor ponds Responses of invasive silver and bighead carp to a carbon dioxide barrier in outdoor ponds
Resource managers need for effective methods to prevent the movement of silver (Hypophthalmichthys molitrix) and bighead carp (H. nobilis) from the Mississippi River basin into the Laurentian Great Lakes. In this study, we evaluated dissolved carbon dioxide (CO2) as a barrier and deterrent to silver (278 ± 30.5 mm) and bighead (212 ± 7.7 mm) carp movement in continuous-flow outdoor ponds...
Authors
Aaron Cupp, Richard Erickson, Kim T. Fredricks, Nicholas Swyers, Tyson Hatton, Jon Amberg
Digestive physiology comparisons of aquatic invertebrates in the Upper Mississippi River Basin Digestive physiology comparisons of aquatic invertebrates in the Upper Mississippi River Basin
Limited information is available on the composition of digestive enzymes present in unionid mussels and the zebra mussel, Dreissena polymorpha. Available information is nearly exclusive to species used for culture purposes. A commercially available enzyme assay kit was used to examine the effect of habitat within an ecosystem, season, and species on the activities of several digestive...
Authors
Blake W. Sauey, Jon Amberg, Scott Cooper, Sandra Grunwald, Roger Haro, Mark P. Gaikowski
Carbon dioxide as a tool to deter the movement of invasive bigheaded carps Carbon dioxide as a tool to deter the movement of invasive bigheaded carps
Nonnative bigheaded carps are established in the Mississippi River and there is substantial concern about their potential entry into the interconnected Laurentian Great Lakes. While electrical barriers currently exist as a preventative measure, there is need for additional control mechanisms to promote barrier security through redundancy. We tested the effectiveness of infused carbon...
Authors
Michael Donaldson, Jon Amberg, Shivani Adhikari, Aaron Cupp, Nathan Jensen, Jason Romine, Adam Wright, Mark P. Gaikowski, Cory D. Suski
Environmental DNA calibration study interim technical review report December 2014 Environmental DNA calibration study interim technical review report December 2014
The Environmental DNA Calibration Study (ECALS) is a multi-year study to improve the understanding and interpretation of the detection of Asian carp DNA in environmental samples (eDNA) used in early detection monitoring. eDNA surveillance programs seek to detect the presence of genetic material (DNA in cells sloughed off in slime, feces, urine, etc.) extracted from water samples; the...
Authors
Kelly Baerwaldt, Meredith Bartron, Kurt Schilling, Debbie Lee, Edmond Russo, Trudy Estes, Richard Fischer, Beth Fleming, Michael Guilfoyle, Jack Killgore, Richard Lance, Edward Perkins, Martin Schultz, David Smith, Jon Amberg, Duane Chapman, Mark Gaikowski, Katy Klymus, Catherine Richter
Preliminary characterization of digestive enzymes in freshwater mussels Preliminary characterization of digestive enzymes in freshwater mussels
Resource managers lack an effective chemical tool to control the invasive zebra mussel Dreissena polymorpha. Zebra mussels clog water intakes for hydroelectric companies, harm unionid mussel species, and are believed to be a reservoir of avian botulism. Little is known about the digestive physiology of zebra mussels and unionid mussels. The enzymatic profile of the digestive glands of...
Authors
Blake W. Sauey, Jon Amberg, Scott Cooper, Sandra Grunwald, Teresa Newton, Roger Haro
Improving efficiency and reliability of environmental DNA analysis for silver carp Improving efficiency and reliability of environmental DNA analysis for silver carp
Natural resource agencies have established surveillance programs which use environmental DNA (eDNA) for the early detection of bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix before they establish populations within the Great Lakes. This molecular monitoring technique must be highly accurate and precise for confident interpretation and also efficient...
Authors
Jon J. Amberg, S. McCalla, Emy Monroe, Richard Lance, Kelly Baerwaldt, Mark P. Gaikowski
Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites
The performance of newly developed New Zealand mudsnail (Potamopyrgus antipodarum; NZMS) genetic markers for environmental (eDNA) analysis of water were compared across two laboratories. The genetic markers were tested in four quantitative polymerase chain reaction assays targeting two regions of the NZMS mitochondrial genome, specifically the cytochrome c oxidase subunit 1 (coi) and...
Authors
Christopher Merkes, Keith Turnquist, Christopher Rees, Jon J. Amberg
Environmental DNA calibration study. Interim technical review report Environmental DNA calibration study. Interim technical review report
Invasive aquatic nuisance species pose a major threat to aquatic ecosystems worldwide. Invasive Asian carps, including bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix) have been steadily dispersing upstream through the Mississippi, Illinois, and Des Plaines Rivers since the 1990s. To prevent further movement up the Illinois River into the Chicago Area Waterway...
Authors
K. Baerwaldt, Meredith Bartron, K. Schilling, Debbie Lee, Edmond Russo, Trudy Estes, Richard Fischer, Beth Fleming, Michael Guilfoyle, K. Kilgore, Richard Lance, Edward Perkins, Martin Schultz, David Smith, Jon J. Amberg, Duane Chapman, Mark Gaikowski, Katy Klymus, Cathy Richter
Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data
The prevention of non-indigenous aquatic invasive species spreading into new areas is a goal of many resource managers. New techniques have been developed to survey for species that are difficult to capture with conventional gears that involve the detection of their DNA in water samples (eDNA). This technique is currently used to track the invasion of bigheaded carps (silver carp and...
Authors
Christopher M. Merkes, S. Grace McCalla, Nathan Jensen, Mark Gaikowski, Jon J. Amberg
Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system
To better understand potential diet overlap among exotic Asian species of carp and native species of filter-feeding fishes of the upper Mississippi River system, microscopy was used to document morphological differences in the gill rakers. Analysing samples first with light microscopy and subsequently with confocal microscopy, the three-dimensional structure of gill rakers in...
Authors
Liza R. Walleser, D.R. Howard, Mark Sandheinrich, Mark Gaikowski, Jon Amberg
Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers
Improved management of invasive Silver Carp Hypophthalmichthys molitrix in the upper Mississippi River basin may be possible by better understanding the feeding abilities of this population. Food collection for filter-feeding fishes, such as Silver Carp, is influenced by the species-specific structure of their gill rakers. To investigate structural variation in gill rakers of Silver Carp...
Authors
Liza Walleser, Mark Sandheinrich, David Howard, Mark Gaikowski, Jon Amberg
Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish
Gut microbiota of invasive Asian silver carp (SVCP) and indigenous planktivorous gizzard shad (GZSD) in Mississippi river basin were compared using 16S rRNA gene pyrosequencing. Analysis of more than 440 000 quality-filtered sequences obtained from the foregut and hindgut of GZSD and SVCP revealed high microbial diversity in these samples. GZSD hindgut (GZSD_H) samples (n=23) with >7000...
Authors
Lin Ye, Jon J. Amberg, Duane Chapman, Mark Gaikowski, Wen-Tso Liu
Science and Products
Filter Total Items: 14
Filter Total Items: 53
Responses of invasive silver and bighead carp to a carbon dioxide barrier in outdoor ponds Responses of invasive silver and bighead carp to a carbon dioxide barrier in outdoor ponds
Resource managers need for effective methods to prevent the movement of silver (Hypophthalmichthys molitrix) and bighead carp (H. nobilis) from the Mississippi River basin into the Laurentian Great Lakes. In this study, we evaluated dissolved carbon dioxide (CO2) as a barrier and deterrent to silver (278 ± 30.5 mm) and bighead (212 ± 7.7 mm) carp movement in continuous-flow outdoor ponds...
Authors
Aaron Cupp, Richard Erickson, Kim T. Fredricks, Nicholas Swyers, Tyson Hatton, Jon Amberg
Digestive physiology comparisons of aquatic invertebrates in the Upper Mississippi River Basin Digestive physiology comparisons of aquatic invertebrates in the Upper Mississippi River Basin
Limited information is available on the composition of digestive enzymes present in unionid mussels and the zebra mussel, Dreissena polymorpha. Available information is nearly exclusive to species used for culture purposes. A commercially available enzyme assay kit was used to examine the effect of habitat within an ecosystem, season, and species on the activities of several digestive...
Authors
Blake W. Sauey, Jon Amberg, Scott Cooper, Sandra Grunwald, Roger Haro, Mark P. Gaikowski
Carbon dioxide as a tool to deter the movement of invasive bigheaded carps Carbon dioxide as a tool to deter the movement of invasive bigheaded carps
Nonnative bigheaded carps are established in the Mississippi River and there is substantial concern about their potential entry into the interconnected Laurentian Great Lakes. While electrical barriers currently exist as a preventative measure, there is need for additional control mechanisms to promote barrier security through redundancy. We tested the effectiveness of infused carbon...
Authors
Michael Donaldson, Jon Amberg, Shivani Adhikari, Aaron Cupp, Nathan Jensen, Jason Romine, Adam Wright, Mark P. Gaikowski, Cory D. Suski
Environmental DNA calibration study interim technical review report December 2014 Environmental DNA calibration study interim technical review report December 2014
The Environmental DNA Calibration Study (ECALS) is a multi-year study to improve the understanding and interpretation of the detection of Asian carp DNA in environmental samples (eDNA) used in early detection monitoring. eDNA surveillance programs seek to detect the presence of genetic material (DNA in cells sloughed off in slime, feces, urine, etc.) extracted from water samples; the...
Authors
Kelly Baerwaldt, Meredith Bartron, Kurt Schilling, Debbie Lee, Edmond Russo, Trudy Estes, Richard Fischer, Beth Fleming, Michael Guilfoyle, Jack Killgore, Richard Lance, Edward Perkins, Martin Schultz, David Smith, Jon Amberg, Duane Chapman, Mark Gaikowski, Katy Klymus, Catherine Richter
Preliminary characterization of digestive enzymes in freshwater mussels Preliminary characterization of digestive enzymes in freshwater mussels
Resource managers lack an effective chemical tool to control the invasive zebra mussel Dreissena polymorpha. Zebra mussels clog water intakes for hydroelectric companies, harm unionid mussel species, and are believed to be a reservoir of avian botulism. Little is known about the digestive physiology of zebra mussels and unionid mussels. The enzymatic profile of the digestive glands of...
Authors
Blake W. Sauey, Jon Amberg, Scott Cooper, Sandra Grunwald, Teresa Newton, Roger Haro
Improving efficiency and reliability of environmental DNA analysis for silver carp Improving efficiency and reliability of environmental DNA analysis for silver carp
Natural resource agencies have established surveillance programs which use environmental DNA (eDNA) for the early detection of bighead carp Hypophthalmichthys nobilis and silver carp Hypophthalmichthys molitrix before they establish populations within the Great Lakes. This molecular monitoring technique must be highly accurate and precise for confident interpretation and also efficient...
Authors
Jon J. Amberg, S. McCalla, Emy Monroe, Richard Lance, Kelly Baerwaldt, Mark P. Gaikowski
Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites Validation of eDNA markers for New Zealand mudsnail surveillance and initial eDNA monitoring at Mississippi River Basin sites
The performance of newly developed New Zealand mudsnail (Potamopyrgus antipodarum; NZMS) genetic markers for environmental (eDNA) analysis of water were compared across two laboratories. The genetic markers were tested in four quantitative polymerase chain reaction assays targeting two regions of the NZMS mitochondrial genome, specifically the cytochrome c oxidase subunit 1 (coi) and...
Authors
Christopher Merkes, Keith Turnquist, Christopher Rees, Jon J. Amberg
Environmental DNA calibration study. Interim technical review report Environmental DNA calibration study. Interim technical review report
Invasive aquatic nuisance species pose a major threat to aquatic ecosystems worldwide. Invasive Asian carps, including bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix) have been steadily dispersing upstream through the Mississippi, Illinois, and Des Plaines Rivers since the 1990s. To prevent further movement up the Illinois River into the Chicago Area Waterway...
Authors
K. Baerwaldt, Meredith Bartron, K. Schilling, Debbie Lee, Edmond Russo, Trudy Estes, Richard Fischer, Beth Fleming, Michael Guilfoyle, K. Kilgore, Richard Lance, Edward Perkins, Martin Schultz, David Smith, Jon J. Amberg, Duane Chapman, Mark Gaikowski, Katy Klymus, Cathy Richter
Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data Persistence of DNA in carcasses, slime and avian feces may affect interpretation of environmental DNA data
The prevention of non-indigenous aquatic invasive species spreading into new areas is a goal of many resource managers. New techniques have been developed to survey for species that are difficult to capture with conventional gears that involve the detection of their DNA in water samples (eDNA). This technique is currently used to track the invasion of bigheaded carps (silver carp and...
Authors
Christopher M. Merkes, S. Grace McCalla, Nathan Jensen, Mark Gaikowski, Jon J. Amberg
Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system Confocal microscopy as a useful approach to describe gill rakers of Asian species of carp and native filter-feeding fishes of the upper Mississippi River system
To better understand potential diet overlap among exotic Asian species of carp and native species of filter-feeding fishes of the upper Mississippi River system, microscopy was used to document morphological differences in the gill rakers. Analysing samples first with light microscopy and subsequently with confocal microscopy, the three-dimensional structure of gill rakers in...
Authors
Liza R. Walleser, D.R. Howard, Mark Sandheinrich, Mark Gaikowski, Jon Amberg
Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers Spatial and temporal variation of the gill rakers of gizzard shad and silver carp in three Midwestern rivers
Improved management of invasive Silver Carp Hypophthalmichthys molitrix in the upper Mississippi River basin may be possible by better understanding the feeding abilities of this population. Food collection for filter-feeding fishes, such as Silver Carp, is influenced by the species-specific structure of their gill rakers. To investigate structural variation in gill rakers of Silver Carp...
Authors
Liza Walleser, Mark Sandheinrich, David Howard, Mark Gaikowski, Jon Amberg
Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish
Gut microbiota of invasive Asian silver carp (SVCP) and indigenous planktivorous gizzard shad (GZSD) in Mississippi river basin were compared using 16S rRNA gene pyrosequencing. Analysis of more than 440 000 quality-filtered sequences obtained from the foregut and hindgut of GZSD and SVCP revealed high microbial diversity in these samples. GZSD hindgut (GZSD_H) samples (n=23) with >7000...
Authors
Lin Ye, Jon J. Amberg, Duane Chapman, Mark Gaikowski, Wen-Tso Liu