These images show the hyphae and spores of the fungus that causes white-nose syndrome (in green) invading the lab grown skin cells of little brown bats. (Isidoro-Ayza, 2024, Science)
Marcos Isidoro-Ayza
Marcos Isidoro-Ayza is a Wildlife Pathologist and Researcher at the National Wildlife Health Center.
He is a DVM, PhD in Comparative Biomedical Sciences, and a Diplomate of the European College of Veterinary Pathologists (ECVP).
Marcos' research focuses on wildlife pathology, infectious diseases, comparative immunology and epithelial biology, and tissue repair, with particular expertise in white-nose syndrome of bats.
Science and Products
Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data
(i) This dataset presents macroscopic and microscopic pathological findings and bacteriology of 178 tadpoles diagnosed with Severe Perkinsea Infections. (ii) Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (iii) Necropsies and gross...
Natural infections with Pigeon Paramyxovirus-1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the USA: Data Natural infections with Pigeon Paramyxovirus-1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the USA: Data
We reviewed pathological findings and to a lesser extent epidemiological data from 70 free-ranging columbiforms naturally infected with Pigeon paramyxovirus-1 (PPMV-1) from 25 different PPMV-1 mortality events in columbiforms in the USA. In a subset of 17 birds from 10 of the studied outbreaks, we carried out immunohistochemistry targeting PPMV-1 nucleoprotein to determine the tissue...
The skin I live in: Pathogenesis of white-nose syndrome of bats The skin I live in: Pathogenesis of white-nose syndrome of bats
The emergence of white-nose syndrome (WNS) in North America has resulted in mass mortalities of hibernating bats and total extirpation of local populations. The need to mitigate this disease has stirred a significant body of research to understand its pathogenesis. Pseudogymnoascus destructans, the causative agent of WNS, is a psychrophilic (cold-loving) fungus that resides within the...
Authors
Marcos Isidoro-Ayza, Jeffrey M. Lorch, Bruce S. Klein
Evidence of vertical transmission of the snake fungal pathogen Ophidiomyces ophiodiicola Evidence of vertical transmission of the snake fungal pathogen Ophidiomyces ophiodiicola
Snake fungal disease (ophidiomycosis) is an emerging infection of snakes caused by Ophidiomyces ophiodiicola. Little is known about mechanisms of this pathogen's transmission and its implications for conservation of wild snake populations. We report four cases with evidence of vertical transmission of O. ophiodiicola from dam to offspring.
Authors
Anne G Stengle, Terence M. Farrell, Keenan S Freitas, Craig M. Lind, Steven J. Price, Brian O Butler, Tigran Tadevosyan, Marcos Isidoro Ayza, Daniel Taylor, Megan Winzeler, Jeffrey M. Lorch
Interactive effects of food supplementation and snake fungal disease on pregnant Pygmy Rattlesnakes and their offspring Interactive effects of food supplementation and snake fungal disease on pregnant Pygmy Rattlesnakes and their offspring
In viviparous organisms, the ability to feed while pregnant may mitigate energetic trade-offs experienced during the reproductive process and enhance fecundity. However, anorexia during pregnancy has been reported in many crotaline snakes. The potential costs and benefits of feeding while pregnant are not completely described in the literature, and experimental studies have been...
Authors
Craig M. Lind, Amber Clark, Sarah A Smiley-Walters, Daniel Taylor, Marcos Isidoro Ayza, Jeffrey M. Lorch, Terence M. Farrell
Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus) Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)
White-nose syndrome (WNS) caused by the fungus, Pseudogymnoascus destructans (Pd) has killed millions of North American hibernating bats. Currently, methods to prevent the disease are limited. We conducted two trials to assess potential WNS vaccine candidates in wild-caught Myotis lucifugus. In a pilot study, we immunized bats with one of four vaccine treatments or phosphate-buffered...
Authors
Tonie E. Rocke, Brock Kingstad-Bakke, Marcel Wuthrich, Ben Stading, Rachel C. Abbott, Marcos Isidoro Ayza, Hannah E. Dobson, Lucas dos Santos Dias, Kevin Galles, Julia S. Lankton, Elizabeth Falendysz, Jeffrey M. Lorch, J. Scott Fites, Jaime Lopera-Madrid, Bruce Klein, Jorge E. Osorio, J. Paul White
Pathology and case definition of Severe Perkinsea Infections of frogs Pathology and case definition of Severe Perkinsea Infections of frogs
Severe Perkinsea infection (SPI) is an emerging disease of frogs responsible for mass mortalities of tadpoles across the United States. It is caused by protozoa belonging to the phylum Perkinsozoa that form a distinct group referred to as the Pathogenic Perkinsea Clade of frogs. In this work, we provide detailed description of gross and histologic lesions from 178 naturally infected...
Authors
Marcos Isidoro Ayza, Daniel A. Grear, Aurelie Chambouvet
Mortality of little brown bats (Myotis lucifugus carissima) naturally exposed to microcystin-LR Mortality of little brown bats (Myotis lucifugus carissima) naturally exposed to microcystin-LR
We describe a die-off of little brown bats (Myotis lucifugus carissima) associated with acute intoxication with microcystin-LR in 2016 at Scofield Reservoir in Utah. High levels of this cyanotoxin in water from the reservoir and gastrointestinal content of bats supported this diagnosis.
Authors
Marcos Isidoro Ayza, Lee C. Jones, Robert J. Dusek, Jeffrey M. Lorch, Jan H. Landsberg, Patrick Wilson, Stephanie Graham
Mortality trends in northern sea otters (Enhydra lutris kenyoni) collected from the coasts of Washington and Oregon (2002–15) Mortality trends in northern sea otters (Enhydra lutris kenyoni) collected from the coasts of Washington and Oregon (2002–15)
During 2002−15 we examined the causes of mortality in a population of northern sea otters (Enhydra lutris kenyoni). Beachcast sea otters were collected primarily from the coast of Washington. Although there are no permanent sea otter residents in Oregon, several beachcast otters were collected from the Oregon coast. Infectious diseases were the primary cause of death (56%) for otters we...
Authors
C. LeAnn White, Emily W. Lankau, Deanna Lynch, Susan Knowles, Krysten L. Schuler, Jitender P. Dubey, Valerie I. Shearn-Bochsler, Marcos Isidoro Ayza, Nancy J. Thomas
Pathogenic lineage of Perkinsea associated with mass mortality of frogs across the United States Pathogenic lineage of Perkinsea associated with mass mortality of frogs across the United States
Emerging infectious diseases such as chytridiomycosis and ranavirus infections are important contributors to the worldwide decline of amphibian populations. We reviewed data on 247 anuran mortality events in 43 States of the United States from 1999–2015. Our findings suggest that a severe infectious disease of tadpoles caused by a protist belonging to the phylum Perkinsea might represent...
Authors
Marcos Isidoro Ayza, Jeffrey M. Lorch, Daniel A. Grear, Megan Winzeler, Daniel L. Calhoun, William J. Barichivich
Natural infections with pigeon paramyxovirus serotype 1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the United States Natural infections with pigeon paramyxovirus serotype 1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the United States
Pigeon paramyxovirus serotype 1 (PPMV-1) is a globally distributed, virulent member of the avian paramyxovirus serotype 1 serogroup that causes mortality in columbiformes and poultry. Following introduction into the United States in the mid-1980s, PPMV-1 rapidly spread causing numerous mortality events in Eurasian collared-doves (Streptopelia decaocto) (ECDOs) and rock pigeons (Columba...
Authors
Marcos Isidoro Ayza, C.L. Afonso, J.B. Stanton, Susan Knowles, S. Ip, C. LeAnn White, Heather Fenton, M.G. Ruder, A. C. Dolinski, Julia S. Lankton
WNS_Fungus
These images show the hyphae and spores of the fungus that causes white-nose syndrome (in green) invading the lab grown skin cells of little brown bats. (Isidoro-Ayza, 2024, Science)
Science and Products
Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data Pathology and bacteriology of 178 tadpoles with histologically confirmed Severe Perkinsea Infections: Data
(i) This dataset presents macroscopic and microscopic pathological findings and bacteriology of 178 tadpoles diagnosed with Severe Perkinsea Infections. (ii) Specimen were collected and submitted to the NWHC as part of mortality investigations and collection of specimen from apparently healthy populations as part of ongoing amphibian health monitoring. (iii) Necropsies and gross...
Natural infections with Pigeon Paramyxovirus-1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the USA: Data Natural infections with Pigeon Paramyxovirus-1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the USA: Data
We reviewed pathological findings and to a lesser extent epidemiological data from 70 free-ranging columbiforms naturally infected with Pigeon paramyxovirus-1 (PPMV-1) from 25 different PPMV-1 mortality events in columbiforms in the USA. In a subset of 17 birds from 10 of the studied outbreaks, we carried out immunohistochemistry targeting PPMV-1 nucleoprotein to determine the tissue...
The skin I live in: Pathogenesis of white-nose syndrome of bats The skin I live in: Pathogenesis of white-nose syndrome of bats
The emergence of white-nose syndrome (WNS) in North America has resulted in mass mortalities of hibernating bats and total extirpation of local populations. The need to mitigate this disease has stirred a significant body of research to understand its pathogenesis. Pseudogymnoascus destructans, the causative agent of WNS, is a psychrophilic (cold-loving) fungus that resides within the...
Authors
Marcos Isidoro-Ayza, Jeffrey M. Lorch, Bruce S. Klein
Evidence of vertical transmission of the snake fungal pathogen Ophidiomyces ophiodiicola Evidence of vertical transmission of the snake fungal pathogen Ophidiomyces ophiodiicola
Snake fungal disease (ophidiomycosis) is an emerging infection of snakes caused by Ophidiomyces ophiodiicola. Little is known about mechanisms of this pathogen's transmission and its implications for conservation of wild snake populations. We report four cases with evidence of vertical transmission of O. ophiodiicola from dam to offspring.
Authors
Anne G Stengle, Terence M. Farrell, Keenan S Freitas, Craig M. Lind, Steven J. Price, Brian O Butler, Tigran Tadevosyan, Marcos Isidoro Ayza, Daniel Taylor, Megan Winzeler, Jeffrey M. Lorch
Interactive effects of food supplementation and snake fungal disease on pregnant Pygmy Rattlesnakes and their offspring Interactive effects of food supplementation and snake fungal disease on pregnant Pygmy Rattlesnakes and their offspring
In viviparous organisms, the ability to feed while pregnant may mitigate energetic trade-offs experienced during the reproductive process and enhance fecundity. However, anorexia during pregnancy has been reported in many crotaline snakes. The potential costs and benefits of feeding while pregnant are not completely described in the literature, and experimental studies have been...
Authors
Craig M. Lind, Amber Clark, Sarah A Smiley-Walters, Daniel Taylor, Marcos Isidoro Ayza, Jeffrey M. Lorch, Terence M. Farrell
Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus) Virally-vectored vaccine candidates against white-nose syndrome induce anti-fungal immune response in little brown bats (Myotis lucifugus)
White-nose syndrome (WNS) caused by the fungus, Pseudogymnoascus destructans (Pd) has killed millions of North American hibernating bats. Currently, methods to prevent the disease are limited. We conducted two trials to assess potential WNS vaccine candidates in wild-caught Myotis lucifugus. In a pilot study, we immunized bats with one of four vaccine treatments or phosphate-buffered...
Authors
Tonie E. Rocke, Brock Kingstad-Bakke, Marcel Wuthrich, Ben Stading, Rachel C. Abbott, Marcos Isidoro Ayza, Hannah E. Dobson, Lucas dos Santos Dias, Kevin Galles, Julia S. Lankton, Elizabeth Falendysz, Jeffrey M. Lorch, J. Scott Fites, Jaime Lopera-Madrid, Bruce Klein, Jorge E. Osorio, J. Paul White
Pathology and case definition of Severe Perkinsea Infections of frogs Pathology and case definition of Severe Perkinsea Infections of frogs
Severe Perkinsea infection (SPI) is an emerging disease of frogs responsible for mass mortalities of tadpoles across the United States. It is caused by protozoa belonging to the phylum Perkinsozoa that form a distinct group referred to as the Pathogenic Perkinsea Clade of frogs. In this work, we provide detailed description of gross and histologic lesions from 178 naturally infected...
Authors
Marcos Isidoro Ayza, Daniel A. Grear, Aurelie Chambouvet
Mortality of little brown bats (Myotis lucifugus carissima) naturally exposed to microcystin-LR Mortality of little brown bats (Myotis lucifugus carissima) naturally exposed to microcystin-LR
We describe a die-off of little brown bats (Myotis lucifugus carissima) associated with acute intoxication with microcystin-LR in 2016 at Scofield Reservoir in Utah. High levels of this cyanotoxin in water from the reservoir and gastrointestinal content of bats supported this diagnosis.
Authors
Marcos Isidoro Ayza, Lee C. Jones, Robert J. Dusek, Jeffrey M. Lorch, Jan H. Landsberg, Patrick Wilson, Stephanie Graham
Mortality trends in northern sea otters (Enhydra lutris kenyoni) collected from the coasts of Washington and Oregon (2002–15) Mortality trends in northern sea otters (Enhydra lutris kenyoni) collected from the coasts of Washington and Oregon (2002–15)
During 2002−15 we examined the causes of mortality in a population of northern sea otters (Enhydra lutris kenyoni). Beachcast sea otters were collected primarily from the coast of Washington. Although there are no permanent sea otter residents in Oregon, several beachcast otters were collected from the Oregon coast. Infectious diseases were the primary cause of death (56%) for otters we...
Authors
C. LeAnn White, Emily W. Lankau, Deanna Lynch, Susan Knowles, Krysten L. Schuler, Jitender P. Dubey, Valerie I. Shearn-Bochsler, Marcos Isidoro Ayza, Nancy J. Thomas
Pathogenic lineage of Perkinsea associated with mass mortality of frogs across the United States Pathogenic lineage of Perkinsea associated with mass mortality of frogs across the United States
Emerging infectious diseases such as chytridiomycosis and ranavirus infections are important contributors to the worldwide decline of amphibian populations. We reviewed data on 247 anuran mortality events in 43 States of the United States from 1999–2015. Our findings suggest that a severe infectious disease of tadpoles caused by a protist belonging to the phylum Perkinsea might represent...
Authors
Marcos Isidoro Ayza, Jeffrey M. Lorch, Daniel A. Grear, Megan Winzeler, Daniel L. Calhoun, William J. Barichivich
Natural infections with pigeon paramyxovirus serotype 1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the United States Natural infections with pigeon paramyxovirus serotype 1: Pathologic changes in Eurasian collared-doves (Streptopelia decaocto) and rock pigeons (Columba livia) in the United States
Pigeon paramyxovirus serotype 1 (PPMV-1) is a globally distributed, virulent member of the avian paramyxovirus serotype 1 serogroup that causes mortality in columbiformes and poultry. Following introduction into the United States in the mid-1980s, PPMV-1 rapidly spread causing numerous mortality events in Eurasian collared-doves (Streptopelia decaocto) (ECDOs) and rock pigeons (Columba...
Authors
Marcos Isidoro Ayza, C.L. Afonso, J.B. Stanton, Susan Knowles, S. Ip, C. LeAnn White, Heather Fenton, M.G. Ruder, A. C. Dolinski, Julia S. Lankton
WNS_Fungus
These images show the hyphae and spores of the fungus that causes white-nose syndrome (in green) invading the lab grown skin cells of little brown bats. (Isidoro-Ayza, 2024, Science)
These images show the hyphae and spores of the fungus that causes white-nose syndrome (in green) invading the lab grown skin cells of little brown bats. (Isidoro-Ayza, 2024, Science)