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Rassa Draugelis-Dale, Ph.D. (Former Employee)

Science and Products

Fig. 1. A flow cytometric histogram of larval Grass Carp cells

A Website Tool for Predicting Triploidy in Larval Fish Spawns

Differentiating diploids from triploids at the earliest life stage possible allows for a more efficient use of resources including production time and rearing space. Thus, a reliable flow cytometric (FCM) method has been developed to discriminate triploids from diploids at the larval stage. In order to help simplify the process of differentiating triploids from diploids, we propose a simple...
By
Ecosystems Mission Area, Species Management Research Program, Wetland and Aquatic Research Center
A Website Tool for Predicting Triploidy in Larval Fish Spawns

A Website Tool for Predicting Triploidy in Larval Fish Spawns

Differentiating diploids from triploids at the earliest life stage possible allows for a more efficient use of resources including production time and rearing space. Thus, a reliable flow cytometric (FCM) method has been developed to discriminate triploids from diploids at the larval stage. In order to help simplify the process of differentiating triploids from diploids, we propose a simple...
Learn More

Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area (1999-2006) Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area (1999-2006)

Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among sub-basins within LMNRA. To investigate whether environmental contaminant exposure is associated with alterations in male common carp...
By
Wetland and Aquatic Research Center
Filter Total Items: 18

Flow cytometric assessments of metabolic activity in bacterial assemblages provide insight into ecosystem condition along the Buffalo National River, Arkansas Flow cytometric assessments of metabolic activity in bacterial assemblages provide insight into ecosystem condition along the Buffalo National River, Arkansas

The Buffalo National River (BNR), on karst terrain in Arkansas, is considered an extraordinary water resource. Water collected in Spring 2017 along BNR was metagenomically analyzed using 16S rDNA, and for 17 months (5/2017–11/2018), bacterial responses were measured in relation to nutrients sampled along a stretch of BNR near a concentrated animal feed operation (CAFO) on Big Creek...
Authors
Jill Jenkins, Rassa Dale, Nina M. Hoffpauir, Brooke A Baudoin, Caroline Matkin, Lucas Driver, Shawn W Hodges, Bonnie L. Brown
By
Ecosystems Mission Area, Wetland and Aquatic Research Center

Juvenile African clawed frogs (Xenopus laevis) express growth, metamorphosis, mortality, gene expression, and metabolic changes when exposed to thiamethoxam and clothianidin Juvenile African clawed frogs (Xenopus laevis) express growth, metamorphosis, mortality, gene expression, and metabolic changes when exposed to thiamethoxam and clothianidin

Neonicotinoids (NEO) represent the main class of insecticides currently in use, with thiamethoxam (THX) and clothianidin (CLO) primarily applied agriculturally. With few comprehensive studies having been performed with non-target amphibians, the aim was to investigate potential biomarker responses along an adverse outcome pathway of NEO exposure, whereby data were collected on multiple...
Authors
Jill Jenkins, Katherine R. Hartop, Ghadeer Bukhari, Debra E. Howton, Kelly L. Smalling, Scott Mize, Michelle L. Hladik, Darren Johnson, Rassa Dale, Bonnie L. Brown
By
Water Resources Mission Area, Ecosystems Mission Area, California Water Science Center, New Jersey Water Science Center, Wetland and Aquatic Research Center

Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area

Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among the sub-basins within LMNRA. To investigate whether exposure to environmental contaminants is associated with alterations in male common carp...
Authors
Jill A. Jenkins, Michael R. Rosen, Rassa O. Dale, Kathy R. Echols, Leticia Torres, Carla M. Wieser, Constance A. Kersten, S. Goodbred
By
Ecosystems Mission Area, Columbia Environmental Research Center, Wetland and Aquatic Research Center

An accurate method for measuring triploidy of larval fish spawns An accurate method for measuring triploidy of larval fish spawns

A standard flow cytometric protocol was developed for estimating triploid induction in batches of larval fish. Polyploid induction treatments are not guaranteed to be 100% efficient, thus the ability to quantify the proportion of triploid larvae generated by a particular treatment helps managers to stock high-percentage spawns and researchers to select treatments for efficient triploid...
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Robert Glennon, Anita M. Kelly, Bonnie L. Brown, John Morrison
By
Ecosystems Mission Area, Wetland and Aquatic Research Center

Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens) Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)

Declining harvests of yellow perch, Perca flavescens, in urbanized watersheds of Chesapeake Bay have prompted investigations of their reproductive fitness. The purpose of this study was to establish a flow cytometric technique for DNA analysis of fixed samples sent from the field to provide reliable gamete quality measurements. Similar to the sperm chromatin structure assay, measures...
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Alfred E. Pinkney, Luke R. Iwanowicz, Vicki Blazer
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Wetland and Aquatic Research Center

Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Persistent organochlorine pollutants such as polychlorinated biphenyls (PCBs), dichlorodiphenyldichloroethylene (p,p′-DDE), and polybrominated diphenyl ethers (PBDEs) are stable, bioaccumulative, and widely found in the environment, wildlife, and the human population. To explore the hypothesis that reproduction in male fish is associated with environmental exposures in the lower Columbia...
Authors
Jill A. Jenkins, H.M. Olivier, R. O. Draugelis-Dale, B.E. Eilts, L. Torres, R. Patiño, Elena B. Nilsen, Steven L. Goodbred
By
Ecosystems Mission Area, Wetland and Aquatic Research Center

Science and Products

Fig. 1. A flow cytometric histogram of larval Grass Carp cells

A Website Tool for Predicting Triploidy in Larval Fish Spawns

Differentiating diploids from triploids at the earliest life stage possible allows for a more efficient use of resources including production time and rearing space. Thus, a reliable flow cytometric (FCM) method has been developed to discriminate triploids from diploids at the larval stage. In order to help simplify the process of differentiating triploids from diploids, we propose a simple...
By
Ecosystems Mission Area, Species Management Research Program, Wetland and Aquatic Research Center
A Website Tool for Predicting Triploidy in Larval Fish Spawns

A Website Tool for Predicting Triploidy in Larval Fish Spawns

Differentiating diploids from triploids at the earliest life stage possible allows for a more efficient use of resources including production time and rearing space. Thus, a reliable flow cytometric (FCM) method has been developed to discriminate triploids from diploids at the larval stage. In order to help simplify the process of differentiating triploids from diploids, we propose a simple...
Learn More

Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area (1999-2006) Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area (1999-2006)

Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among sub-basins within LMNRA. To investigate whether environmental contaminant exposure is associated with alterations in male common carp...
By
Wetland and Aquatic Research Center
Filter Total Items: 18

Flow cytometric assessments of metabolic activity in bacterial assemblages provide insight into ecosystem condition along the Buffalo National River, Arkansas Flow cytometric assessments of metabolic activity in bacterial assemblages provide insight into ecosystem condition along the Buffalo National River, Arkansas

The Buffalo National River (BNR), on karst terrain in Arkansas, is considered an extraordinary water resource. Water collected in Spring 2017 along BNR was metagenomically analyzed using 16S rDNA, and for 17 months (5/2017–11/2018), bacterial responses were measured in relation to nutrients sampled along a stretch of BNR near a concentrated animal feed operation (CAFO) on Big Creek...
Authors
Jill Jenkins, Rassa Dale, Nina M. Hoffpauir, Brooke A Baudoin, Caroline Matkin, Lucas Driver, Shawn W Hodges, Bonnie L. Brown
By
Ecosystems Mission Area, Wetland and Aquatic Research Center

Juvenile African clawed frogs (Xenopus laevis) express growth, metamorphosis, mortality, gene expression, and metabolic changes when exposed to thiamethoxam and clothianidin Juvenile African clawed frogs (Xenopus laevis) express growth, metamorphosis, mortality, gene expression, and metabolic changes when exposed to thiamethoxam and clothianidin

Neonicotinoids (NEO) represent the main class of insecticides currently in use, with thiamethoxam (THX) and clothianidin (CLO) primarily applied agriculturally. With few comprehensive studies having been performed with non-target amphibians, the aim was to investigate potential biomarker responses along an adverse outcome pathway of NEO exposure, whereby data were collected on multiple...
Authors
Jill Jenkins, Katherine R. Hartop, Ghadeer Bukhari, Debra E. Howton, Kelly L. Smalling, Scott Mize, Michelle L. Hladik, Darren Johnson, Rassa Dale, Bonnie L. Brown
By
Water Resources Mission Area, Ecosystems Mission Area, California Water Science Center, New Jersey Water Science Center, Wetland and Aquatic Research Center

Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area Sperm quality biomarkers complement reproductive and endocrine parameters in investigating environmental contaminants in common carp (Cyprinus carpio) from the Lake Mead National Recreation Area

Lake Mead National Recreational Area (LMNRA) serves as critical habitat for several federally listed species and supplies water for municipal, domestic, and agricultural use in the Southwestern U.S. Contaminant sources and concentrations vary among the sub-basins within LMNRA. To investigate whether exposure to environmental contaminants is associated with alterations in male common carp...
Authors
Jill A. Jenkins, Michael R. Rosen, Rassa O. Dale, Kathy R. Echols, Leticia Torres, Carla M. Wieser, Constance A. Kersten, S. Goodbred
By
Ecosystems Mission Area, Columbia Environmental Research Center, Wetland and Aquatic Research Center

An accurate method for measuring triploidy of larval fish spawns An accurate method for measuring triploidy of larval fish spawns

A standard flow cytometric protocol was developed for estimating triploid induction in batches of larval fish. Polyploid induction treatments are not guaranteed to be 100% efficient, thus the ability to quantify the proportion of triploid larvae generated by a particular treatment helps managers to stock high-percentage spawns and researchers to select treatments for efficient triploid...
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Robert Glennon, Anita M. Kelly, Bonnie L. Brown, John Morrison
By
Ecosystems Mission Area, Wetland and Aquatic Research Center

Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens) Flow cytometric method for measuring chromatin fragmentation in fixed sperm from yellow perch (Perca flavescens)

Declining harvests of yellow perch, Perca flavescens, in urbanized watersheds of Chesapeake Bay have prompted investigations of their reproductive fitness. The purpose of this study was to establish a flow cytometric technique for DNA analysis of fixed samples sent from the field to provide reliable gamete quality measurements. Similar to the sperm chromatin structure assay, measures...
Authors
Jill A. Jenkins, Rassa O. Draugelis-Dale, Alfred E. Pinkney, Luke R. Iwanowicz, Vicki Blazer
By
Ecosystems Mission Area, Contaminant Biology, Environmental Health Program, Wetland and Aquatic Research Center

Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Persistent organochlorine pollutants such as polychlorinated biphenyls (PCBs), dichlorodiphenyldichloroethylene (p,p′-DDE), and polybrominated diphenyl ethers (PBDEs) are stable, bioaccumulative, and widely found in the environment, wildlife, and the human population. To explore the hypothesis that reproduction in male fish is associated with environmental exposures in the lower Columbia...
Authors
Jill A. Jenkins, H.M. Olivier, R. O. Draugelis-Dale, B.E. Eilts, L. Torres, R. Patiño, Elena B. Nilsen, Steven L. Goodbred
By
Ecosystems Mission Area, Wetland and Aquatic Research Center
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