Robert Bonde, Ph.D. (Former Employee)

Mammals diversified by colonizing drastically different environments, with each transition yielding numerous molecular changes, including losses of protein function. Though not initially deleterious, these losses could subsequently carry deleterious pleiotropic consequences. We have used phylogenetic methods to identify convergent functional losses across independent marine mammal...

The Florida manatee (Trichechus manatus latirostris) has limited diversity in the immunoglobulin heavy chain. We therefore investigated the antigen receptor loci of the other arm of the adaptive immune system: the T cell receptor. Manatees are the first species from Afrotheria, a basal eutherian superorder, to have an in-depth characterization of all T cell receptor loci. By annotating...

Sirenians, including the manatees and dugongs, are large herbivorous mammals that have evolved to an aquatic form since the Eocene epoch. Sirenians have unique adaptations, including dense bone for ballast and a longitudinal hemidiaphragm separating paired lungs (which aid in maintaining a horizontal posture in the water column), species-specific rostral deflection, and unique dentition...

Toxoplasma gondii is a feline protozoan reported to cause morbidity and mortality in manatees and other marine mammals. Given the herbivorous nature of manatees, ingestion of oocysts from contaminated water or seagrass is presumed to be their primary mode of infection. The objectives of this study were to investigate oocyst contamination of seagrass beds in Puerto Rico and determine the

Manatees are a vulnerable, charismatic sentinel species from the evolutionarily divergent Afrotheria. Manatee health and resistance to infectious disease is of great concern to conservation groups, but little is known about their immune system. To develop manatee-specific tools for monitoring health, we first must have a general knowledge of how the immunoglobulin heavy (IgH) chain locus...

Assessing the welfare of wild populations of sirenians has required a “generalist” approach. The outcome has been a subjective decision as to whether what the observers are witnessing in an individual or group of animals is normal and whether that has positive or negative consequences. The understanding of sirenian welfare requirements, and a decision process for whether to support and...

There are three extant sirenian species of the Trichechidae family and one living Dugongidae family member. Given their close ties to coastal and often urbanized habitats, sirenians are exposed to many types of anthropogenic activities that result in challenges to their well-being, poor health, and even death. In the wild, they are exposed to direct and indirect local pressures as well...

Belize contains important habitat for Antillean manatees (Trichechus manatus manatus) and provides refuge for the highest known population density of this subspecies. As these animals face impending threats, knowledge of their dietary habits can be used to interpret resource utilization. The contents of 13 mouth, 6 digestive tract (stomach, duodenum and colon), and 124 fecal samples were

In many species, body weight (W) increases geometrically with body length (L), so W/L3 provides a body condition index (BCI) that can be used to evaluate nutritional status once a normal range has been established. No such index has been established for Florida manatees (Trichechus manatus latirostris). This study was designed to determine a normal range of BCIs of Florida manatees by...

The West Indian manatee (Trichechus manatus) is exposed to a number of anthropogenic influences, including metals, as they inhabit shallow waters with close proximity to shore. While maintaining homeostasis of many metals is crucial for health, there is currently no baseline reference range that can be used to make clinical and environmental decisions for this endangered species. In this...

Seasonal changes in light, temperature, and food availability stimulate a physiological response in an animal. Seasonal adaptations are well studied in Arctic, Sub-Arctic, and hibernating mammals; however, limited studies have been conducted in sub-tropical species. The Florida manatee (Trichechus manatus latirostris), a sub-tropical marine mammal, forages less during colder temperatures...

The rescue, rehabilitation, and release of Florida manatees (Trichechus manatus latirostris) into the wild has occurred since 1974; however, a comprehensive evaluation of the outcomes of the releases has never been conducted. Herein, we examined data for 136 Florida manatees that were rehabilitated and released with telemetry tags between 1988 and 2013 to determine release outcome of...