The Florida manatee (Trichechus manatus latirostris) has limited diversity in the immunoglobulin heavy chain. We therefore investigated the antigen receptor loci of the other arm of the adaptive immune system: the T cell receptor. Manatees are the first species from Afrotheria, a basal eutherian superorder, to have an in-depth characterization of all T cell receptor loci. By annotating the genome and expressed transcripts, we found that each chain has distinct features that correlates to their individual functions. The genomic organization also plays a role in modulating sequence conservation between species. There were extensive V subgroup synteny blocks in the TRA and TRB loci between T. m. latirostrisand human. Increased genomic locus complexity correlated to increased locus synteny. We also identified evidence for a VHD pseudogene for the first time in a eutherian mammal. These findings emphasize the value of including species within this basal eutherian radiation in comparative studies.
|Title||The Florida manatee (Trichechus manatus latirostris) T cell receptor loci exhibit V subgroup synteny and chain-specific evolution|
|Authors||Breanna Breaux, Margaret Hunter, Maria Paula Cruz-Schneider, Leonardo Sena, Robert K. Bonde, Michael F. Criscitiello|
|Publication Subtype||Journal Article|
|Series Title||Developmental and Comparative Immunology|
|Record Source||USGS Publications Warehouse|
|USGS Organization||Wetland and Aquatic Research Center|