Luke Iwanowicz processing samples under the BSC within the BSL-3 lab on a 36-sample day.
Robin Johnson
Robin is a Biologist at the Eastern Ecological Science Center in Kearneysville, WV.
Science and Products
Conservation Genetics and Genomics of the Narrowly Endemic Island Marble Butterfly: Assessing Population Structure, Facilitating Supportive Breeding, and Identifying Adaptive Potential Conservation Genetics and Genomics of the Narrowly Endemic Island Marble Butterfly: Assessing Population Structure, Facilitating Supportive Breeding, and Identifying Adaptive Potential
The purpose of this study was to collect and analyze non-invasive DNA specimens from the endangered island marble butterfly (Euchloe ausonides insulanus) to assess population structure, identify adaptive potential, facilitate captive propagation, and support translocation planning. We used DNA extracted from meconium, exuviae, and natural mortalities to generate and test thirteen new
Individual assignments and microsatellite genotypes for Atlantic Sturgeon from 2021 (ver. 4.0, September 2025) Individual assignments and microsatellite genotypes for Atlantic Sturgeon from 2021 (ver. 4.0, September 2025)
This dataset includes genotypes and individual-based assignment tests for 1394 Atlantic Sturgeon selected for assignment by the National Marine Fisheries Service to meet management requirements under the Endangered Species Act.
SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021 SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021
Wastewater-based epidemiology (WBE) is used to determine the consumption of, or exposure to, chemicals or pathogens in human populations, and is conducted by collecting representative samples of untreated wastewater (influent) to quantify pathogens shed in the population served by the sampled wastewater system. The Centers for Disease Control and Prevention (CDC) and the US Department of...
Genotypes of Atlantic Sturgeon collected from Canada to Georgia used in the development of a genetic baseline Genotypes of Atlantic Sturgeon collected from Canada to Georgia used in the development of a genetic baseline
This dataset includes microsatellite genotypes for 2510 Atlantic sturgeon from 18 different populations distributed from Canada to Georgia. Each individual was genotyped at 12 loci. Additional information about sex, capture date, and size are also provided.
Metagenetic analysis of stream community composition based on environmental DNA Metagenetic analysis of stream community composition based on environmental DNA
A survey of environmental DNA was performed in Tunison Creek downstream of the USGS Tunison Aquatic Laboratory. The goal of the survey was to characterize the source ecological community at multiple trophic or taxonomic levels by associating DNA fragments with reference databases. Three taxonomically informative genetic loci were used: the mitochondrial cytochrome oxidase 1 locus, the
Heavy Sample Processing Day
Luke Iwanowicz processing samples under the BSC within the BSL-3 lab on a 36-sample day.
Filter Total Items: 17
Stock composition of cryptic Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) in the Restigouche River and estuary, Canada Stock composition of cryptic Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) in the Restigouche River and estuary, Canada
The Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) is an important species in eastern North America due to its conservation status and cultural significance. Local Mi'gmaw fishers have reported an increase in sturgeon sightings over the last decade in the Restigouche River and estuary. Mi’gmaw Knowledge, oral history, and archaeological finds attest to their historical presence in...
Authors
N.J. Daigle, Shannon L. White, Barbara A. Lubinski, Robin L. Johnson, David C. Kazyak, C.E. Verhille, C.A. Gillis, C.F.D. Sacobie
Spatiotemporal patterns in habitat use of natal and non-natal adult Atlantic sturgeon in two spawning rivers Spatiotemporal patterns in habitat use of natal and non-natal adult Atlantic sturgeon in two spawning rivers
Background Monitoring movement across an organism’s ontogeny is often challenging, particularly for long-lived or wide-ranging species. When empirical data are unavailable, general knowledge about species’ ecology may be used to make assumptions about habitat use across space or time. However, inferences about habitat use based on population-level ecology may overlook important eco...
Authors
Shannon L. White, Matthew W. Breece, Dewayne A. Fox, David C. Kazyak, Amanda Higgs, Ian A Park, Cassia Busch, Barbara A. Lubinski, Robin L. Johnson, Amy Welsh
Integrating genetic and demographic data to refine indices of abundance for Atlantic sturgeon in the Hudson River, New York Integrating genetic and demographic data to refine indices of abundance for Atlantic sturgeon in the Hudson River, New York
Critical to Atlantic sturgeon Acipenser oxyrinchus oxyrinchus recovery and monitoring is the ability to estimate abundance and identify age- and stock-specific threats to survival. As adult Atlantic sturgeon spend much of their lives broadly distributed in marine and estuarine environments, it is challenging to collect data needed to estimate these demographic parameters in the adult...
Authors
Shannon L. White, Richard M. Pendleton, Amanda Higgs, Barbara A. Lubinski, Robin L. Johnson, David C. Kazyak
Validation of a molecular sex marker in three sturgeons from eastern North America Validation of a molecular sex marker in three sturgeons from eastern North America
Despite the importance of sex-specific information for sturgeon conservation and management, sex identification has been a major challenge outside of mature adults on spawning grounds. Recent work identified a sex-specific locus (AllWSex2) that appears to be broadly conserved across many Acipenserids, but the assay was not validated for all species within the family. We tested the...
Authors
Nicholas M Sard, Brian R Krieser, Richard M. Pendleton, Barbara A. Lubinski, Robin L. Johnson, Dewayne A. Fox, Joel P Van Eenennaam, Jason E Kahn, Chris H Hager, Amanda L. Higgs, David C. Kazyak
Genetic population assignments of Atlantic sturgeon provided to National Marine Fisheries Service, 2022 Genetic population assignments of Atlantic sturgeon provided to National Marine Fisheries Service, 2022
Acipenser oxyrinchus oxyrinchus (Atlantic sturgeon) were once abundant and supported large-scale fisheries throughout much of the east coast of the United States. However, historic overharvest and habitat loss resulted in dramatic declines in abundance and eventual listing under the Endangered Species Act of the United States. As part of this listing, Atlantic sturgeon populations were...
Authors
Shannon L. White, Robin L. Johnson, Barbara A. Lubinski, Michael S. Eackles, David C. Kazyak
Decades of global sturgeon conservation efforts are threatened by an expanding captive culture industry Decades of global sturgeon conservation efforts are threatened by an expanding captive culture industry
After centuries of overexploitation and habitat loss, many of the world's sturgeon (Acipenseridae) populations are at the brink of extinction. Although significant resources are invested into the conservation and restoration of imperiled sturgeons, the burgeoning commercial culture industry poses an imminent threat to the persistence of many populations. In the past decade, the number...
Authors
Shannon L. White, Dewayne A. Fox, Tamar Beridze, Stephania K Bolden, Robin L. Johnson, Thomas F Savoy, Fleur Scheele, Andrea D Schreier, David C. Kazyak
Science and Products
Conservation Genetics and Genomics of the Narrowly Endemic Island Marble Butterfly: Assessing Population Structure, Facilitating Supportive Breeding, and Identifying Adaptive Potential Conservation Genetics and Genomics of the Narrowly Endemic Island Marble Butterfly: Assessing Population Structure, Facilitating Supportive Breeding, and Identifying Adaptive Potential
The purpose of this study was to collect and analyze non-invasive DNA specimens from the endangered island marble butterfly (Euchloe ausonides insulanus) to assess population structure, identify adaptive potential, facilitate captive propagation, and support translocation planning. We used DNA extracted from meconium, exuviae, and natural mortalities to generate and test thirteen new
Individual assignments and microsatellite genotypes for Atlantic Sturgeon from 2021 (ver. 4.0, September 2025) Individual assignments and microsatellite genotypes for Atlantic Sturgeon from 2021 (ver. 4.0, September 2025)
This dataset includes genotypes and individual-based assignment tests for 1394 Atlantic Sturgeon selected for assignment by the National Marine Fisheries Service to meet management requirements under the Endangered Species Act.
SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021 SARS-CoV-2 Data from National Wastewater Surveillance System Surge Capacity Sampling, September 2021
Wastewater-based epidemiology (WBE) is used to determine the consumption of, or exposure to, chemicals or pathogens in human populations, and is conducted by collecting representative samples of untreated wastewater (influent) to quantify pathogens shed in the population served by the sampled wastewater system. The Centers for Disease Control and Prevention (CDC) and the US Department of...
Genotypes of Atlantic Sturgeon collected from Canada to Georgia used in the development of a genetic baseline Genotypes of Atlantic Sturgeon collected from Canada to Georgia used in the development of a genetic baseline
This dataset includes microsatellite genotypes for 2510 Atlantic sturgeon from 18 different populations distributed from Canada to Georgia. Each individual was genotyped at 12 loci. Additional information about sex, capture date, and size are also provided.
Metagenetic analysis of stream community composition based on environmental DNA Metagenetic analysis of stream community composition based on environmental DNA
A survey of environmental DNA was performed in Tunison Creek downstream of the USGS Tunison Aquatic Laboratory. The goal of the survey was to characterize the source ecological community at multiple trophic or taxonomic levels by associating DNA fragments with reference databases. Three taxonomically informative genetic loci were used: the mitochondrial cytochrome oxidase 1 locus, the
Heavy Sample Processing Day
Luke Iwanowicz processing samples under the BSC within the BSL-3 lab on a 36-sample day.
Luke Iwanowicz processing samples under the BSC within the BSL-3 lab on a 36-sample day.
Filter Total Items: 17
Stock composition of cryptic Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) in the Restigouche River and estuary, Canada Stock composition of cryptic Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) in the Restigouche River and estuary, Canada
The Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) is an important species in eastern North America due to its conservation status and cultural significance. Local Mi'gmaw fishers have reported an increase in sturgeon sightings over the last decade in the Restigouche River and estuary. Mi’gmaw Knowledge, oral history, and archaeological finds attest to their historical presence in...
Authors
N.J. Daigle, Shannon L. White, Barbara A. Lubinski, Robin L. Johnson, David C. Kazyak, C.E. Verhille, C.A. Gillis, C.F.D. Sacobie
Spatiotemporal patterns in habitat use of natal and non-natal adult Atlantic sturgeon in two spawning rivers Spatiotemporal patterns in habitat use of natal and non-natal adult Atlantic sturgeon in two spawning rivers
Background Monitoring movement across an organism’s ontogeny is often challenging, particularly for long-lived or wide-ranging species. When empirical data are unavailable, general knowledge about species’ ecology may be used to make assumptions about habitat use across space or time. However, inferences about habitat use based on population-level ecology may overlook important eco...
Authors
Shannon L. White, Matthew W. Breece, Dewayne A. Fox, David C. Kazyak, Amanda Higgs, Ian A Park, Cassia Busch, Barbara A. Lubinski, Robin L. Johnson, Amy Welsh
Integrating genetic and demographic data to refine indices of abundance for Atlantic sturgeon in the Hudson River, New York Integrating genetic and demographic data to refine indices of abundance for Atlantic sturgeon in the Hudson River, New York
Critical to Atlantic sturgeon Acipenser oxyrinchus oxyrinchus recovery and monitoring is the ability to estimate abundance and identify age- and stock-specific threats to survival. As adult Atlantic sturgeon spend much of their lives broadly distributed in marine and estuarine environments, it is challenging to collect data needed to estimate these demographic parameters in the adult...
Authors
Shannon L. White, Richard M. Pendleton, Amanda Higgs, Barbara A. Lubinski, Robin L. Johnson, David C. Kazyak
Validation of a molecular sex marker in three sturgeons from eastern North America Validation of a molecular sex marker in three sturgeons from eastern North America
Despite the importance of sex-specific information for sturgeon conservation and management, sex identification has been a major challenge outside of mature adults on spawning grounds. Recent work identified a sex-specific locus (AllWSex2) that appears to be broadly conserved across many Acipenserids, but the assay was not validated for all species within the family. We tested the...
Authors
Nicholas M Sard, Brian R Krieser, Richard M. Pendleton, Barbara A. Lubinski, Robin L. Johnson, Dewayne A. Fox, Joel P Van Eenennaam, Jason E Kahn, Chris H Hager, Amanda L. Higgs, David C. Kazyak
Genetic population assignments of Atlantic sturgeon provided to National Marine Fisheries Service, 2022 Genetic population assignments of Atlantic sturgeon provided to National Marine Fisheries Service, 2022
Acipenser oxyrinchus oxyrinchus (Atlantic sturgeon) were once abundant and supported large-scale fisheries throughout much of the east coast of the United States. However, historic overharvest and habitat loss resulted in dramatic declines in abundance and eventual listing under the Endangered Species Act of the United States. As part of this listing, Atlantic sturgeon populations were...
Authors
Shannon L. White, Robin L. Johnson, Barbara A. Lubinski, Michael S. Eackles, David C. Kazyak
Decades of global sturgeon conservation efforts are threatened by an expanding captive culture industry Decades of global sturgeon conservation efforts are threatened by an expanding captive culture industry
After centuries of overexploitation and habitat loss, many of the world's sturgeon (Acipenseridae) populations are at the brink of extinction. Although significant resources are invested into the conservation and restoration of imperiled sturgeons, the burgeoning commercial culture industry poses an imminent threat to the persistence of many populations. In the past decade, the number...
Authors
Shannon L. White, Dewayne A. Fox, Tamar Beridze, Stephania K Bolden, Robin L. Johnson, Thomas F Savoy, Fleur Scheele, Andrea D Schreier, David C. Kazyak