Metagenetic analysis of stream community composition based on environmental DNA
December 12, 2018
A survey of environmental DNA was performed in Tunison Creek downstream of the USGS Tunison Aquatic Laboratory. The goal of the survey was to characterize the source ecological community at multiple trophic or taxonomic levels by associating DNA fragments with reference databases. Three taxonomically informative genetic loci were used: the mitochondrial cytochrome oxidase 1 locus, the mitochondrial 12S ribosomal locus, and the bacterial/organellar 16S ribosomal locus. The data set includes a list of sample and sequence accessions residing in the National Center for Biotechnology Information databases, which provide the raw data for analysis or re-use. The data also includes the representative sequences of operational taxonomic units (OTUs) and their abundance and taxonomic assignment, which are the primary endpoints of the data collection.
Citation Information
Publication Year | 2018 |
---|---|
Title | Metagenetic analysis of stream community composition based on environmental DNA |
DOI | 10.5066/P93NIUYM |
Authors | Robert S Cornman, James E McKenna Jr, Jennifer Fike, Sara J Oyler-McCance, Robin Johnson |
Product Type | Data Release |
Record Source | USGS Asset Identifier Service (AIS) |
USGS Organization | Fort Collins Science Center |
Rights | This work is marked with CC0 1.0 Universal |
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Robert S. Cornman, James Duncan Mckenna, Jennifer A. Fike, Sara J. Oyler-McCance, Robin L. Johnson
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Related
An experimental comparison of composite and grab sampling of stream water for metagenetic analysis of environmental DNA
Use of environmental DNA (eDNA) to assess distributions of aquatic and semi-aquatic macroorganisms is promising, but sampling schemes may need to be tailored to specific objectives. Given the potentially high variance in aquatic eDNA among replicate grab samples, compositing smaller water volumes collected over a period of time may be more effective for some applications. In this study...
Authors
Robert S. Cornman, James Duncan Mckenna, Jennifer A. Fike, Sara J. Oyler-McCance, Robin L. Johnson
Sara J Oyler-McCance, PhD
Branch Chief / Supervisory Research Geneticist
Branch Chief / Supervisory Research Geneticist
Phone
Ext
197