Stacie A Kageyama, PhD
Stacie A Kageyama
Science and Products
Detection of Dakota skipper eDNA from inflorescences in the Upper Midwest, June and July 2022 (ver. 1.1, November 2025) Detection of Dakota skipper eDNA from inflorescences in the Upper Midwest, June and July 2022 (ver. 1.1, November 2025)
This dataset contains environmental DNA (eDNA) data for two separate tests of a novel quantitative polymerase chain reaction (qPCR) assay for detection of Dakota skipper (Hesperia dacotae) from inflorescences. One test was conducted in a controlled setting, and the other was conducted in a field setting. Also included are qPCR data for negative controls and standard dilutions of Dakota...
qPCR Results for An Assessment of Pathogens in National Park Hot Springs qPCR Results for An Assessment of Pathogens in National Park Hot Springs
In 2018 and 2019, a multidisciplinary team from the U.S. Geological Survey (USGS), National Park Service, The Commonwealth Scientific and Industrial Research Organization, Montana State University’s Department of Microbiology and Immunology, and the Teton Conservation District, initiated a study to document the presence or absence of Naegleria fowleri, Giardia, Escherichia coli, and...
Data Release for Validation of portable eDNA detection kit for Asian carps Data Release for Validation of portable eDNA detection kit for Asian carps
This is the qPCR and loop-mediated isothermal amplification (LAMP) data in support of the article "Validation of a portable eDNA detection kit for invasive carps". There are four types of data contained in five files including: limit of detection analysis for each method (qPCR and LAMP), qPCR analysis and LAMP analysis of eDNA samples collected over time from ponds containing 3 or 33...
Analysis of Grass Carp eDNA Data Analysis of Grass Carp eDNA Data
This product contains code to analyze a dataset that was collected to compare a LAMP assay and a qPCR assay for Grass Carp in a pond study. This repository contains the RMarkdown and R files used to analyze the data. It was created to allow for reproducible results. The code assumes the user is experience with R, especially the Tidyverse, and Stan.
Assessment of carbon dioxide piscicide treatments: Data Assessment of carbon dioxide piscicide treatments: Data
Spreadsheets include data that was used to evaluate the efficacy of dry ice administered under-ice as a tool for reducing the overwinter survival of invasive carps. There are 10 CSV associated with this study
A 21st Century butterfly net: Using eDNA to detect the imperiled Dakota skipper A 21st Century butterfly net: Using eDNA to detect the imperiled Dakota skipper
The development of environmental DNA (eDNA) methods for terrestrial arthropods could be transformative for the difficult task of assessing the status of species of conservation concern. The primary goal of this study was to investigate the efficacy of detecting the Dakota skipper (Hesperia dacotae) from its DNA left behind on inflorescences as a means of inferring species presence. We...
Authors
David Pilliod, Michaela Grossklaus, Stacie Kageyama, Cale Nordmeyer, Jerry Reinisch, Erik Runquist, Stephen Spear
Assessing the suitability of YY males and ZZ females as an invasive species population control method across life histories Assessing the suitability of YY males and ZZ females as an invasive species population control method across life histories
Natural resource managers use tools to control invasive species. In theory, stocking YY males or ZZ females would allow managers to skew sex ratios until populations collapse. In combination with other suppression methods, such as removal, this approach could be incorporated into Integrated Pest Management plans. For example, fishery managers have stocked YY males to control isolated non...
Authors
Richard Erickson, Hannah Thompson, Stacie Kageyama, Grace Andriacchi, Aaron Cupp, Reynaldo Patino, Jon Amberg
Validation of a portable eDNA detection kit for invasive carps Validation of a portable eDNA detection kit for invasive carps
Loop-mediated isothermal amplification (LAMP) is a rapid molecular detection technique that has been used as a diagnostic tool for detecting human and animal pathogens for over 20 years and is promising for detecting environmental DNA shed by invasive species. We designed a LAMP assay to detect the invasive carps, silver carp (Hypophthalmichthys molitrix), bighead carp...
Authors
Stacie Kageyama, Matthew Hoogland, Tariq Tajjioui, Theresa Schreier, Richard Erickson, Christopher Merkes
Assessment of carbon dioxide piscicide treatments Assessment of carbon dioxide piscicide treatments
Few chemicals are approved to control or eradicate nuisance fish populations in the United States. Carbon dioxide (CO2) is currently being developed and studied as a new piscicide option for nonselective population control. This study evaluated dry ice (solid state CO2) as a simple CO2 delivery method during winter piscicide applications. Nonnative Silver Carp Hypophthalmichthys molitrix...
Authors
Aaron Cupp, Justin Smerud, John Tix, Jose Rivera, Stacie Kageyama, Christopher Merkes, Richard Erickson, Jon Amberg, Mark P. Gaikowski
Spiked qPCR primary analysis Spiked qPCR primary analysis
This script is for analyzing raw eDNA qPCR data. It is designed to efficiently concatenate qPCR data from multiple plates exported as *.csv files. It will assist with completing several QA/QC checks and output documentation of these checks as well as helpful files for further review.
Analysis of Grass Carp eDNA Data Analysis of Grass Carp eDNA Data
This product contains code to analyze a dataset that was collected to compare a LAMP assay and a qPCR assay for Grass Carp in a pond study. This repository contains the RMarkdown and R files used to analyze the data. It was created to allow for reproducible results. The code assumes the user is experience with R, especially the Tidyverse, and Stan.
Science and Products
Detection of Dakota skipper eDNA from inflorescences in the Upper Midwest, June and July 2022 (ver. 1.1, November 2025) Detection of Dakota skipper eDNA from inflorescences in the Upper Midwest, June and July 2022 (ver. 1.1, November 2025)
This dataset contains environmental DNA (eDNA) data for two separate tests of a novel quantitative polymerase chain reaction (qPCR) assay for detection of Dakota skipper (Hesperia dacotae) from inflorescences. One test was conducted in a controlled setting, and the other was conducted in a field setting. Also included are qPCR data for negative controls and standard dilutions of Dakota...
qPCR Results for An Assessment of Pathogens in National Park Hot Springs qPCR Results for An Assessment of Pathogens in National Park Hot Springs
In 2018 and 2019, a multidisciplinary team from the U.S. Geological Survey (USGS), National Park Service, The Commonwealth Scientific and Industrial Research Organization, Montana State University’s Department of Microbiology and Immunology, and the Teton Conservation District, initiated a study to document the presence or absence of Naegleria fowleri, Giardia, Escherichia coli, and...
Data Release for Validation of portable eDNA detection kit for Asian carps Data Release for Validation of portable eDNA detection kit for Asian carps
This is the qPCR and loop-mediated isothermal amplification (LAMP) data in support of the article "Validation of a portable eDNA detection kit for invasive carps". There are four types of data contained in five files including: limit of detection analysis for each method (qPCR and LAMP), qPCR analysis and LAMP analysis of eDNA samples collected over time from ponds containing 3 or 33...
Analysis of Grass Carp eDNA Data Analysis of Grass Carp eDNA Data
This product contains code to analyze a dataset that was collected to compare a LAMP assay and a qPCR assay for Grass Carp in a pond study. This repository contains the RMarkdown and R files used to analyze the data. It was created to allow for reproducible results. The code assumes the user is experience with R, especially the Tidyverse, and Stan.
Assessment of carbon dioxide piscicide treatments: Data Assessment of carbon dioxide piscicide treatments: Data
Spreadsheets include data that was used to evaluate the efficacy of dry ice administered under-ice as a tool for reducing the overwinter survival of invasive carps. There are 10 CSV associated with this study
A 21st Century butterfly net: Using eDNA to detect the imperiled Dakota skipper A 21st Century butterfly net: Using eDNA to detect the imperiled Dakota skipper
The development of environmental DNA (eDNA) methods for terrestrial arthropods could be transformative for the difficult task of assessing the status of species of conservation concern. The primary goal of this study was to investigate the efficacy of detecting the Dakota skipper (Hesperia dacotae) from its DNA left behind on inflorescences as a means of inferring species presence. We...
Authors
David Pilliod, Michaela Grossklaus, Stacie Kageyama, Cale Nordmeyer, Jerry Reinisch, Erik Runquist, Stephen Spear
Assessing the suitability of YY males and ZZ females as an invasive species population control method across life histories Assessing the suitability of YY males and ZZ females as an invasive species population control method across life histories
Natural resource managers use tools to control invasive species. In theory, stocking YY males or ZZ females would allow managers to skew sex ratios until populations collapse. In combination with other suppression methods, such as removal, this approach could be incorporated into Integrated Pest Management plans. For example, fishery managers have stocked YY males to control isolated non...
Authors
Richard Erickson, Hannah Thompson, Stacie Kageyama, Grace Andriacchi, Aaron Cupp, Reynaldo Patino, Jon Amberg
Validation of a portable eDNA detection kit for invasive carps Validation of a portable eDNA detection kit for invasive carps
Loop-mediated isothermal amplification (LAMP) is a rapid molecular detection technique that has been used as a diagnostic tool for detecting human and animal pathogens for over 20 years and is promising for detecting environmental DNA shed by invasive species. We designed a LAMP assay to detect the invasive carps, silver carp (Hypophthalmichthys molitrix), bighead carp...
Authors
Stacie Kageyama, Matthew Hoogland, Tariq Tajjioui, Theresa Schreier, Richard Erickson, Christopher Merkes
Assessment of carbon dioxide piscicide treatments Assessment of carbon dioxide piscicide treatments
Few chemicals are approved to control or eradicate nuisance fish populations in the United States. Carbon dioxide (CO2) is currently being developed and studied as a new piscicide option for nonselective population control. This study evaluated dry ice (solid state CO2) as a simple CO2 delivery method during winter piscicide applications. Nonnative Silver Carp Hypophthalmichthys molitrix...
Authors
Aaron Cupp, Justin Smerud, John Tix, Jose Rivera, Stacie Kageyama, Christopher Merkes, Richard Erickson, Jon Amberg, Mark P. Gaikowski
Spiked qPCR primary analysis Spiked qPCR primary analysis
This script is for analyzing raw eDNA qPCR data. It is designed to efficiently concatenate qPCR data from multiple plates exported as *.csv files. It will assist with completing several QA/QC checks and output documentation of these checks as well as helpful files for further review.
Analysis of Grass Carp eDNA Data Analysis of Grass Carp eDNA Data
This product contains code to analyze a dataset that was collected to compare a LAMP assay and a qPCR assay for Grass Carp in a pond study. This repository contains the RMarkdown and R files used to analyze the data. It was created to allow for reproducible results. The code assumes the user is experience with R, especially the Tidyverse, and Stan.