Fluorescence is an often overlooked property of reef-building corals that can improve the classification of reef habitats from imagery and provide diagnostic information on corals.
Applications of Coral Fluorescence
Fluorescence is a trait expressed by numerous coral reef denizens, including algae, cnidarians, sponges, polychaetes, fish, crustaceans, and mollusks. The source of the fluorescence is organism-dependent. Organisms that conduct photosynthesis, either directly or via endosymbiotic algae, contain chlorophyll, which fluoresces red. Anemones, reef-building corals, and other cnidarians often contain additional pigments in their epithelial cells that fluoresce at a variety of wavelengths, corresponding to colors from cyan to red. These fluorescent pigments are homologs of the green fluorescent protein (GFP) first isolated in the north Pacific jellyfish Aequorea victoria. The function and ecological significance of GFPs in corals remains an open question and an area of active research. Most efforts have been focused on the diversity, biochemical structure, and possible physiological role of GFPs in corals.
The purpose of this task is to evaluate the potential of fluorescence as an investigative tool for two applications: classifying coral reef habitats and identifying disease-induced physiological stress.

Improving Seabed Classification with Fluorescence
Basic classification of Caribbean seabed features (e.g., live coral, dead coral, rubble, sand, and algae) is difficult with conventional reflectance-based remote sensing. Fluorescence spectra may provide additional distinguishing features to improve classification results. A modeling experiment using a constrained set of fluorescence spectra representing 15 ecological functional groups common to Caribbean reefs yielded an average classification accuracy of 87%. For details, see PLoS ONE article "Fluorescence-Based Classification of Caribbean Coral Reef Organisms and Substrates."
Diagnosing Coral Disease
Mitigation of impacts from coral diseases and syndromes requires better diagnostic techniques to detect the onset of disease. Ideally, these techniques would be rapid and non-destructive. Prior work (Changes in the fluorescence of the Caribbean coral Montastraea faveolata during heat-induced bleaching) has shown that the same GFP fluorescent emissions that may aid classification can be used as a proxy for temperature-induced stress in corals. Analyzing the differences between diseased and healthy specimens, might enable the development of an index relating fluorescence to the onset of disease or precursors to it. Such a diagnostic tool would provide researchers and resource managers with a rapid, non- invasive means to assess coral health.
Below are other science projects associated with this research.
Advanced Remote Sensing Methods for Coastal Science and Management
Modeling Seafloor Structural Complexity
Along-Track Reef Imaging System (ATRIS)
Below are publications associated with this researcg.
Fluorescence-based classification of Caribbean coral reef organisms and substrates
Applying New Methods to Diagnose Coral Diseases
- Overview
Fluorescence is an often overlooked property of reef-building corals that can improve the classification of reef habitats from imagery and provide diagnostic information on corals.
Applications of Coral Fluorescence
Fluorescence is a trait expressed by numerous coral reef denizens, including algae, cnidarians, sponges, polychaetes, fish, crustaceans, and mollusks. The source of the fluorescence is organism-dependent. Organisms that conduct photosynthesis, either directly or via endosymbiotic algae, contain chlorophyll, which fluoresces red. Anemones, reef-building corals, and other cnidarians often contain additional pigments in their epithelial cells that fluoresce at a variety of wavelengths, corresponding to colors from cyan to red. These fluorescent pigments are homologs of the green fluorescent protein (GFP) first isolated in the north Pacific jellyfish Aequorea victoria. The function and ecological significance of GFPs in corals remains an open question and an area of active research. Most efforts have been focused on the diversity, biochemical structure, and possible physiological role of GFPs in corals.
The purpose of this task is to evaluate the potential of fluorescence as an investigative tool for two applications: classifying coral reef habitats and identifying disease-induced physiological stress.
Fluorescence endmember library. Based on empirical measurements, most fluorescent signals observed on Caribbean reefs are attributable to one or more of these spectra. Sources/Usage: Public Domain. Visit Media to see details.Fluorescence functional groups. These 15 spectra denote the most common fluorescent emissions measured on Caribbean reefs. Note: All of the stony coral functional groups (1-7) also include a chlorophyll component. Fluorescence intensities are plotted in arbitrary units, and the spectra indicate typical peak-to-peak ratios. See PLoS ONE article for details. Improving Seabed Classification with Fluorescence
Basic classification of Caribbean seabed features (e.g., live coral, dead coral, rubble, sand, and algae) is difficult with conventional reflectance-based remote sensing. Fluorescence spectra may provide additional distinguishing features to improve classification results. A modeling experiment using a constrained set of fluorescence spectra representing 15 ecological functional groups common to Caribbean reefs yielded an average classification accuracy of 87%. For details, see PLoS ONE article "Fluorescence-Based Classification of Caribbean Coral Reef Organisms and Substrates."
Diagnosing Coral Disease
Mitigation of impacts from coral diseases and syndromes requires better diagnostic techniques to detect the onset of disease. Ideally, these techniques would be rapid and non-destructive. Prior work (Changes in the fluorescence of the Caribbean coral Montastraea faveolata during heat-induced bleaching) has shown that the same GFP fluorescent emissions that may aid classification can be used as a proxy for temperature-induced stress in corals. Analyzing the differences between diseased and healthy specimens, might enable the development of an index relating fluorescence to the onset of disease or precursors to it. Such a diagnostic tool would provide researchers and resource managers with a rapid, non- invasive means to assess coral health.
Daylight and fluorescent images of a Montastrea cavernosa coral colony growing on the seawall at Ft. Jefferson, Dry Tortugas National Park. The brownish color of the coral in daylight is largely attributable to chlorophyll contained within the symbiotic algae harbored by the coral. The fluorescent image was taken at night using blue LED lights to stimulate fluorescence. The green color is attributable to a compound called green fluorescent protein (GFP) contained in the coral tissue, not the symbiotic algae. Daylight and fluorescen images of a Orbicella faveolata coral colony in Dry Tortugas National Park. In this colony, the GFP fluorescence is so intense that it overwhelms the weaker red fluorescence of chlorophyll. Daylight and fluorescent images of two coral colonies, Diploria clivosa (left) and Orbicella faveolata (right) growing on the seawall at Ft. Jefferson, Dry Tortugas National Park. The two colonies are separated by an area of dead coral that has been colonized by algae. In the fluorescent image, red corresponds to chlorophyll and green to GFP. Note that the GFP is concentrated in the center of each coral polyp. - Science
Below are other science projects associated with this research.
Advanced Remote Sensing Methods for Coastal Science and Management
This project focused on developing algorithms for quantifying benthic habitat complexity from images, modeling the structural complexity of the seafloor, and using fluorescence signatures to classify coral reef habitats.Modeling Seafloor Structural Complexity
This effort focused on understanding the patterns and scalability of roughness and topographic complexity of marine habitats, such as coral reefs.Along-Track Reef Imaging System (ATRIS)
ATRIS is a benthic-survey tool that simultaneously acquires geo-located, color, digital images with corresponding water depths. - Publications
Below are publications associated with this researcg.
Fluorescence-based classification of Caribbean coral reef organisms and substrates
A diverse group of coral reef organisms, representing several phyla, possess fluorescent pigments. We investigated the potential of using the characteristic fluorescence emission spectra of these pigments to enable unsupervised, optical classification of coral reef habitats. We compiled a library of characteristic fluorescence spectra through in situ and laboratory measurements from a variety of sAuthorsDavid G. Zawada, Charles H. MazelApplying New Methods to Diagnose Coral Diseases
Coral disease, one of the major causes of reef degradation and coral death, has been increasing worldwide since the 1970s, particularly in the Caribbean. Despite increased scientific study, simple questions about the extent of disease outbreaks and the causative agents remain unanswered. A component of the U.S. Geological Survey Coral Reef Ecosystem STudies (USGS CREST) project is focused on develAuthorsChristina A. Kellogg, David G. Zawada