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Publications

Below is a list of available WFRC peer reviewed and published science.

Filter Total Items: 2528

Characterization of a linear DNA plasmid from the filamentous fungal plant pathogen Glomerella musae [Anamorph: Colletotrichum musae (Berk. and Curt.) arx.] Characterization of a linear DNA plasmid from the filamentous fungal plant pathogen Glomerella musae [Anamorph: Colletotrichum musae (Berk. and Curt.) arx.]

A 7.4-kilobase (kb) DNA plasmid was isolated from Glomerella musae isolate 927 and designated pGML1. Exonuclease treatments indicated that pGML1 was a linear plasmid with blocked 5' termini. Cell-fractionation experiments combined with sequence-specific PCR amplification revealed that pGML1 resided in mitochondria. The pGML1 plasmid hybridized to cesium chloride-fractionated nuclear DNA...
Authors
S. Freeman, R. S. Redman, G. Grantham, R. J. Rodriguez

Gas bubble disease monitoring and research of juvenile salmonids Gas bubble disease monitoring and research of juvenile salmonids

No abstract available
Authors
Alec G. Maule, John W. Beeman, K.M. Hans, M. G. Mesa, Philip V. Haner, Joe Warren

Immunogenicity of synthetic peptides representing neutralizing epitopes on the glycoprotein of infectious hematopoietic necrosis virus Immunogenicity of synthetic peptides representing neutralizing epitopes on the glycoprotein of infectious hematopoietic necrosis virus

Three peptides, P76, P226, and P268 representing 3 putative antigen~c determinants on the glycoprotein of infectious hematopoietic necrosis virus (IHNV), were synthesized and injected into rainbow trout Oncorhynchus mykiss to assess their immunogen~city. Antisera extracted from the immunized trout were analyzed uslng an enzyme linked imrnunosorbent assay (ELISA) for the presence of...
Authors
E. Emmenegger, M. Landolt, S. LaPatra, J. Winton

Sequence analysis and expression of the M1 and M2 matrix protein genes of hirame rhabdovirus (HIRRV) Sequence analysis and expression of the M1 and M2 matrix protein genes of hirame rhabdovirus (HIRRV)

We have cloned and sequenced a 2318 nucleotide region of the genomic RNA of hirame rhabdovirus (HIRRV), an important viral pathogen of Japanese flounder Paralichthys olivaceus. This region comprises approximately two-thirds of the 3' end of the nucleocapsid protein (N) gene and the complete matrix protein (M1 and M2) genes with the associated intergenic regions. The partial N gene...
Authors
T. Nishizawa, Gael Kurath, J. R. Winton

Comparison of two fluorescent antibody techniques (FATS) for detection and quantification of Renibacterium salmoninarum in coelomic fluid of spawning chinook salmon Oncorhynchus tshawytscha Comparison of two fluorescent antibody techniques (FATS) for detection and quantification of Renibacterium salmoninarum in coelomic fluid of spawning chinook salmon Oncorhynchus tshawytscha

Two versions of the fluorescent antibody technique (FAT) were compared for detection and quantification of Renibacterium salmoninarum in coelomic fluid samples from naturally infected spawning chinook salmon Oncorhynchus tshawytscha. For the membrane filtration-FAT (MF-FAT), trypsin-treated samples were passed through 0.2 ??m polycarbonate filters to concentrate bacteria for direct...
Authors
D.G. Elliott, C.L. McKibben

Developmental instability analysis of BKD-infected spring Chinook salmon (Onchorhynchus tshawytscha) prior to seawater exposure Developmental instability analysis of BKD-infected spring Chinook salmon (Onchorhynchus tshawytscha) prior to seawater exposure

Stress in organisms results in energy dissipation, making developmental pathways less stable. Effects of chronic stress, manifested as small random departures from phenotypic symmetry, reflect developmental instability, are considered to be epigenetic and an effect produced by compromised fitness. Instability is detectable and effectively interpreted among sites or populations if samples...
Authors
W.B. Campbell, J.M. Emlen

The impact of grazing on plant fractal architecture and fitness of a mediterranean shrub (Anthyllis cytisoides L.) The impact of grazing on plant fractal architecture and fitness of a mediterranean shrub (Anthyllis cytisoides L.)

1. We examined natural grazing by livestock (sheep and goats) on Albaida Anthyllis cytisoides L. with the aim of determining whether variation in the allometric relationships between plant parts provides a sensitive indicator of the impact of grazing. 2. The intra-individual variation in translatory symmetry with scale and increased complexity of fractal structures reflect environmental...
Authors
J. Escos, C.L. Alados, J.M. Emlen
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