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Publications

Below is a list of available WFRC peer reviewed and published science.

Filter Total Items: 2528

Molecular epizootiology and evolution of the glycoprotein and non-virion protein genes of infectious hematopoietic necrosis virus, a fish rhabdovirus Molecular epizootiology and evolution of the glycoprotein and non-virion protein genes of infectious hematopoietic necrosis virus, a fish rhabdovirus

Infectious hematopoietic necrosis virus (IHNV) causes a highly lethal, economically important disease of salmon and trout. The virus is enzootic throughout western North America, and has been spread to Asia and Europe. The nucleotide sequences of the glycoprotein (G) and non-virion (NV) genes of 12 diverse IHNV isolates were determined in order to examine the molecular epizootiology of...
Authors
Stuart T. Nichol, Joan E. Rowe, James R. Winton

A non-lethal measure of smolt status of juvenile steelhead based on body morphology A non-lethal measure of smolt status of juvenile steelhead based on body morphology

A nonlethal morphometric method to assess smolt status of juvenile steelhead Oncorhynchus mykiss was validated. Fish were collected from hatcheries before release and during their seaward migration in a large river system. Fifteen anatomical landmarks were digitized from photographs of each fish, resulting in 34 morphometric characters based on a truss network. Principal component and...
Authors
John W. Beeman, D.W. Rondorf, M.E. Tilson, D.A. Venditti

Inactivation of Renibacterium salmoninarum by free chlorine Inactivation of Renibacterium salmoninarum by free chlorine

Salmonid fishes contract bacterial kidney disease by vertical or horizontal transmission of the pathogenic bacterium, Renibacterium salmoninarum. Procedures to reduce vertical transmission are under evaluation, but methods are still needed to eliminate sources of waterborne R. salmoninarum. We examined the efficacy of chlorine to inactivate R. salmoninarum. The bacterium was exposed to...
Authors
Ronald J. Pascho, Marsha L. Landolt, Jerry E. Ongerth

Development of a DNA probe for the myxosporean parasite, Ceratomyxa shasta, using the polymerase chain reaction with arbitrary primers Development of a DNA probe for the myxosporean parasite, Ceratomyxa shasta, using the polymerase chain reaction with arbitrary primers

The arbitrarily primed polymerase chain reaction (PCR) was used to generate a DNA marker specific for the myxosporean parasite Ceratomyxa shasta. The [32P]-labeled marker hybridized to purified C. shasta DNA and to parasite DNA combined with salmonid DNA in a dot blot assay, demonstrating its potential as a diagnostic tool. The amplified DNA segment was cloned and sequenced, and primers...
Authors
Jerri L Bartholomew, Rusty J. Rodriguez, Cindy K. Arakawa

Brood stock segregation for the control of bacterial kidney disease can affect mortality of progeny chinook salmon (Oncorhynchus tshawytscha) in seawater Brood stock segregation for the control of bacterial kidney disease can affect mortality of progeny chinook salmon (Oncorhynchus tshawytscha) in seawater

Segregation of spring chinook salmon (Oncorhynchus tshawytscha) brood stock based on the measurement of maternal Renibacterium salmoninarum infection levels by the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody technique (FAT) was previously shown to affect the prevalence and levels of bacterial kidney disease (BKD) in progeny fish during hatchery rearing. Smolts...
Authors
Diane G. Elliott, Ronald J. Pascho, Aldo N. Palmisano

The complete genome structure and phylogenetic relationship of infectious hematopoietic necrosis virus The complete genome structure and phylogenetic relationship of infectious hematopoietic necrosis virus

Infectious hematopoietic necrosis virus (IHNV), a member of the family Rhabdoviridae, causes a severe disease with high mortality in salmonid fish. The nucleotide sequence (11, 131 bases) of the entire genome was determined for the pathogenic WRAC strain of IHNV from southern Idaho. This allowed detailed analysis of all 6 genes, the deduced amino acid sequences of their encoded proteins...
Authors
Sergey P. Morzunov, James R. Winton, Stuart T. Nichol
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